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CHAF1B / CAF1

chromatin assembly factor 1, subunit B (p60)

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Gene Name: chromatin assembly factor 1, subunit B (p60)
Synonyms: CHAF1B, CAF-1 subunit B, CAF-I 60 kDa subunit, CAF-I p60, CAF1A, CAF-1, CAF-IP60, CAF1, CAF1P60, M-phase phosphoprotein 7, MPHOSPH7
Target Sequences: NM_005441 NP_005432.1 Q13112

Publications (5)

1
Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro. Smith S, Stillman B. The Journal of biological chemistry. 1991 266:12041-7. [PubMed:2050697]
2
Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Shibahara K, Stillman B. Cell. 1999 96:575-85. [PubMed:10052459]
3
Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. Green CM, Almouzni G. The EMBO journal. 2003 22:5163-74. [PubMed:14517254] [PMC:PMC204462]
4
In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells. Galvani A, Courbeyrette R, Agez M, Ochsenbein F, Mann C, Thuret JY. Molecular and cellular biology. 2008 28:3672-85. [PubMed:18378699] [PMC:PMC2423286]
5
HIRA and Daxx constitute two independent histone H3.3-containing predeposition complexes. Elsaesser SJ, Allis CD. Cold Spring Harbor symposia on quantitative biology. 2010 75:27-34. [PubMed:21047901]
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Proteins (6)
Over-Expression Lysate (2)
Recombinant (4)
CHAF1B / CAF1 (6)
No (6)
GST (1)
GST, N-terminus (1)
His-T7 (1)
Myc-DDK (Flag) (3)
Human (4)
Mouse (1)
293T Cells (1)
E. coli (1)
HEK 293 Cells (2)
Wheat Germ Extract (2)
Purified (1)
CHAF1B / CAF1 Protein - Purified recombinant protein CHAF1B was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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Human CHAF1B / CAF1 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G67780
HEK 293 Cells
Myc-DDK (Flag)
61.3 kDa
20 µg/$1,107
CHAF1B / CAF1 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Human CHAF1B / CAF1 Protein (Recombinant GST, N-terminus) (aa201-300) - LS-G60667
Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
CHAF1B / CAF1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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Human CHAF1B / CAF1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G80708
293T Cells
Myc-DDK (Flag)
61.3 kDa
20 µg/$150
CHAF1B / CAF1 Protein - 12.5% SDS-PAGE of human CHAF1B stained with Coomassie Blue
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Human CHAF1B / CAF1 Protein (Recombinant GST) (Full Length) - LS-G31081
Wheat Germ Extract
GST
87.9 kDa
10 µg/$479; 25 µg/$670
CHAF1B / CAF1 Protein - Recombinant Chromatin Assembly Factor 1, Subunit B By SDS-PAGE
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Mouse CHAF1B / CAF1 Protein (Recombinant His-T7) (aa1-199) - LS-G25994
E. coli
His-T7
26.2 kDa
10 µg/$281; 50 µg/$442; 100 µg/$705; 200 µg/$882; 1 mg/$1,989; 500 µg/$1,529; 5 mg/$3,081; 2 mg/$2,176
CHAF1B / CAF1 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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CHAF1B / CAF1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G100553
HEK 293 Cells
Myc-DDK (Flag)
61.3 kDa
100 µg/$494
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