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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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CHAF1B / CAF1

chromatin assembly factor 1, subunit B (p60)

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Gene Name: chromatin assembly factor 1, subunit B (p60)
Synonyms: CHAF1B, CAF-1 subunit B, CAF-I 60 kDa subunit, CAF-I p60, CAF1A, CAF-1, CAF-IP60, CAF1, CAF1P60, M-phase phosphoprotein 7, MPHOSPH7
Target Sequences: NM_005441 NP_005432.1 Q13112

Publications (5)

1
Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro. Smith S, Stillman B. The Journal of biological chemistry. 1991 266:12041-7. [PubMed:2050697]
2
Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Shibahara K, Stillman B. Cell. 1999 96:575-85. [PubMed:10052459]
3
Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. Green CM, Almouzni G. The EMBO journal. 2003 22:5163-74. [PubMed:14517254] [PMC:PMC204462]
4
In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells. Galvani A, Courbeyrette R, Agez M, Ochsenbein F, Mann C, Thuret JY. Molecular and cellular biology. 2008 28:3672-85. [PubMed:18378699] [PMC:PMC2423286]
5
HIRA and Daxx constitute two independent histone H3.3-containing predeposition complexes. Elsaesser SJ, Allis CD. Cold Spring Harbor symposia on quantitative biology. 2010 75:27-34. [PubMed:21047901]
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Viewing 1-25 of 37 items
CHAF1B / CAF1 Antibody - IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CHAF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Select
Mouse Monoclonal [clone OTI4F7] (IgG1) to Human CHAF1B / CAF1
Mouse, Dog, Rat, Human, Monkey
IF, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $345.00
CHAF1B / CAF1 Antibody - IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CHAF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Select
Mouse Monoclonal [clone OTI4F7] (IgG1) to Human CHAF1B / CAF1
Mouse, Dog, Rat, Human, Monkey
IF, IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
CHAF1B / CAF1 Antibody
Select
Rabbit Polyclonal to Human CHAF1B / CAF1
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $275.00
Select
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
CHAF1B / CAF1 Antibody - Immunohistochemistry of paraffin-embedded mouse kidney tissue.
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Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
20µl   $215.00; 50µl   $235.00; 100µl   $315.00; 200µl   $425.00
CHAF1B / CAF1 Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.
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Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
Select
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
60µl   1mg/ml   $235.00; 120µl   1mg/ml   $295.00; 200µl   1mg/ml   $395.00
CHAF1B / CAF1 Antibody - Western blot of CAF-1 p60 (W93) pAb in extracts from Jurkat cells.
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Rabbit Polyclonal to Human CHAF1B / CAF1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
100µl   $345.00
CHAF1B / CAF1 Antibody - CAF-1 p60 Antibody (SS 24 1-68) -  CAF-1 p60 (SS24 1-68) - Immunocytochemical staining of CAF-1 p60 in HeLa cells (Smith and Stillman 1991).
Select
Mouse Monoclonal [clone SS 24 1-68] (IgG1) to Human CHAF1B / CAF1
Mouse, Human, Primate
ICC, IP, WB
Unconjugated
50µl   $335.00
CHAF1B / CAF1 Antibody - Detection of Human CAF-1 p60 by Immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:200 (1 ug/ml).
Select
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Human
IHC, IP, WB
Unconjugated
100µl   0.2mg/ml   $405.00
CHAF1B / CAF1 Antibody - Anti-CHAF1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAF1B.
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Mouse Monoclonal [clone OTI2D5] (IgG1) to Human CHAF1B / CAF1
Human
IF, WB
Unconjugated
100µl   1mg/ml   $345.00
CHAF1B / CAF1 Antibody - Anti-CHAF1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAF1B.
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Mouse Monoclonal [clone OTI2D5] (IgG1) to Human CHAF1B / CAF1
Human
IF, WB
Unconjugated, Carrier-free
100µg   $395.00
CHAF1B / CAF1 Antibody - CHAF1B antibody CHAF1B(chromatin assembly factor 1, subunit B (p60)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Human
IHC, IHC-P, WB
Unconjugated
100µl   0.5mg/ml   $335.00
CHAF1B / CAF1 Antibody - CHAF1B antibody CHAF1B(chromatin assembly factor 1, subunit B (p60)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Human, Gibbon
IHC, IHC-P, WB
HRP Conjugated
100µl   0.65mg/ml   $355.00
CHAF1B / CAF1 Antibody - CHAF1B antibody CHAF1B(chromatin assembly factor 1, subunit B (p60)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Human, Gibbon
IHC, IHC-P, WB
Biotin Conjugated
100µl   1mg/ml   $355.00
CHAF1B / CAF1 Antibody - CHAF1B antibody CHAF1B(chromatin assembly factor 1, subunit B (p60)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Human, Gibbon
IHC, IHC-P, WB
FITC Conjugated
100µl   1mg/ml   $355.00
CHAF1B / CAF1 Antibody - Detection limit for recombinant GST tagged CHAF1B is 0.1 ng/ml as a capture antibody.
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Mouse Monoclonal [clone 2G7] (IgG2b,k) to Human CHAF1B / CAF1
Human
ELISA, WB
Unconjugated
100µg   $365.00
CHAF1B / CAF1 Antibody
Select
Mouse Monoclonal [clone p60-24] (IgG1) to Human CHAF1B / CAF1
Human
ICC, IP
Unconjugated
100µg   1mg/ml   $335.00
CHAF1B / CAF1 Antibody - Western blot analysis of extracts from Jurkat cells, using CAF1B antibody.
Select
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µg   $305.00
CHAF1B / CAF1 Antibody - CAF-1 p60 (SS53) Antibody - Western Blot of: A. RKO cells with p150 ab, PCNA ab, CHAF1B Antibody CAF-1 p60 antibody B. p150 RNAi knockdown
Select
Mouse Monoclonal [clone SS 53] (IgG1) to Human CHAF1B / CAF1
Human
IP, WB
Unconjugated
100µl   $335.00
CHAF1B / CAF1 Antibody - Western blot analysis of lysates from Jurkat cells, using CAF1B Antibody. The lane on the right is blocked with the synthesized peptide.
Select
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
CHAF1B / CAF1 Antibody - Western blot analysis of the lysates from RAW264.7cells using CAF1B antibody.
Select
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
CHAF1B / CAF1 Antibody - Detection of Human CAF-1 p60 by Immunohistochemistry. Sample: FFPE section of human seminoma. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:250.
Select
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Human
IHC
Unconjugated
100µl   $415.00
CHAF1B / CAF1 Antibody - Western blot of CHAF1B / CAF1 antibody.
Select
Rabbit Polyclonal to Mouse CHAF1B / CAF1
Mouse
WB
Unconjugated
100µl   0.5mg/ml   $265.00; 200µl   0.5mg/ml   $305.00; 500µl   0.5mg/ml   $445.00; 1ml   0.5mg/ml   $545.00
Select
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Rat, Human
WB
Unconjugated
20µl   $215.00; 50µl   $235.00; 100µl   $295.00; 200µl   $385.00

Viewing 1-25 of 37 items


If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).