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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CHAF1B / CAF1

chromatin assembly factor 1, subunit B (p60)

Complex that is thought to mediate chromatin assembly in DNA replication and DNA repair. Assembles histone octamers onto replicating DNA in vitro. CAF-1 performs the first step of the nucleosome assembly process, bringing newly synthesized histones H3 and H4 to replicating DNA; histones H2A/H2B can bind to this chromatin precursor subsequent to DNA replication to complete the histone octamer.

Gene Name: chromatin assembly factor 1, subunit B (p60)
Synonyms: CHAF1B, CAF-1 subunit B, CAF-I 60 kDa subunit, CAF-I p60, CAF1A, CAF-1, CAF-IP60, CAF1, CAF1P60, M-phase phosphoprotein 7, MPHOSPH7
Target Sequences: NM_005441 NP_005432.1 Q13112

Publications (5)

1
Immunological characterization of chromatin assembly factor I, a human cell factor required for chromatin assembly during DNA replication in vitro. Smith S, Stillman B. The Journal of biological chemistry. 1991 266:12041-7. [PubMed:2050697]
2
Replication-dependent marking of DNA by PCNA facilitates CAF-1-coupled inheritance of chromatin. Shibahara K, Stillman B. Cell. 1999 96:575-85. [PubMed:10052459]
3
Local action of the chromatin assembly factor CAF-1 at sites of nucleotide excision repair in vivo. Green CM, Almouzni G. The EMBO journal. 2003 22:5163-74. [PubMed:14517254] [PMC:PMC204462]
4
In vivo study of the nucleosome assembly functions of ASF1 histone chaperones in human cells. Galvani A, Courbeyrette R, Agez M, Ochsenbein F, Mann C, Thuret JY. Molecular and cellular biology. 2008 28:3672-85. [PubMed:18378699] [PMC:PMC2423286]
5
HIRA and Daxx constitute two independent histone H3.3-containing predeposition complexes. Elsaesser SJ, Allis CD. Cold Spring Harbor symposia on quantitative biology. 2010 75:27-34. [PubMed:21047901]
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Filtered by: None
Target
CHAF1B / CAF1 (36)
Reactivity
Human (28)
Mouse (22)
Rat (14)
Bat (4)
Chicken (4)
Chimpanzee (3)
Dog (6)
Gibbon (6)
Guinea pig (4)
Horse (4)
Monkey (6)
Pig (4)
Primate (1)
Rabbit (4)
Xenopus (4)
Application
IHC (13)
IHC-P (6)
WB (33)
ELISA (3)
ICC (3)
IF (10)
IP (5)
Peptide-ELISA (2)
Host
rabbit (28)
mouse (8)
Isotype
IgG (14)
IgG1 (7)
IgG2b,k (1)
Clonality
monoclonal mc (8)
polyclonal pc (28)
Clone
2G7 (1)
OTI2D5 (2)
OTI4F7 (2)
p60-24 (1)
SS 24 1-68 (1)
SS 53 (1)
Format
APC (1)
APC Conjugated (1)
Biotin Conjugated (3)
Carrier-free (2)
Cy3 Conjugated (1)
Cy7 Conjugated (1)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (23)
Epitope
aa1-199 (8)
C-Terminus (4)
N-Terminus (4)
aa509-C-term (2)
40-120 aa, Internal (1)
Trp93 (1)
aa375-425 (1)
aa44-93 (1)
aa71-120 (1)
Publications
No (36)
Showing 1-25 of 36 results

CHAF1B / CAF1 Antibody (clone OTI4F7) LS-C338213
Mouse Monoclonal [clone OTI4F7] (IgG1) to Human CHAF1B / CAF1
Mouse, Dog, Rat, Human, Monkey
IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Carcinoma of Human prostate tissue using anti-CHAF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
IHC of paraffin-embedded Human lymph node tissue using anti-CHAF1B mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).

CHAF1B / CAF1 Antibody (clone OTI4F7, Carrier-free) LS-C788318
Mouse Monoclonal [clone OTI4F7] (IgG1) to Human CHAF1B / CAF1
Mouse, Dog, Rat, Human, Monkey
IF, IHC, WB
Unconjugated, Carrier-free
100µg  $395.00

CHAF1B / CAF1 Antibody LS-C668165
Rabbit Polyclonal to Human CHAF1B / CAF1
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $275.00
Immunohistochemistry - Paraffin Image
Immunofluorescence Image

CHAF1B / CAF1 Antibody (40-120 aa, Internal) LS-C382395
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

CHAF1B / CAF1 Antibody LS-C335645
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded mouse kidney tissue.
Immunohistochemistry of paraffin-embedded rat brain tissue.

CHAF1B / CAF1 Antibody LS-C765492
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

CHAF1B / CAF1 Antibody (Trp93) LS-C177124
Rabbit Polyclonal to Human CHAF1B / CAF1
Mouse, Rat, Human
IF, IHC, WB
Unconjugated
100µl  $345.00
Western blot of CAF-1 p60 (W93) pAb in extracts from Jurkat cells.

CHAF1B / CAF1 Antibody LS-C804537
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
ELISA, IF, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00

CHAF1B / CAF1 Antibody (clone OTI2D5) LS-C338214
Mouse Monoclonal [clone OTI2D5] (IgG1) to Human CHAF1B / CAF1
Human
IF, WB
Unconjugated
100µl 1mg/ml  $345.00
Anti-CHAF1B mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CHAF1B.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CHAF1B (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CHAF1B.

CHAF1B / CAF1 Antibody (clone OTI2D5, Carrier-free) LS-C788320
Mouse Monoclonal [clone OTI2D5] (IgG1) to Human CHAF1B / CAF1
Human
IF, WB
Unconjugated, Carrier-free
100µg  $395.00

CHAF1B / CAF1 Antibody (clone SS 24 1-68) LS-C2626
Mouse Monoclonal [clone SS 24 1-68] (IgG1) to Human CHAF1B / CAF1
Mouse, Human, Primate
ICC, IP, WB
Unconjugated
50µl  $335.00
CAF-1 p60 Antibody (SS 24 1-68) -  CAF-1 p60 (SS24 1-68) - Immunocytochemical staining of CAF-1 p60 in HeLa cells (Smith and Stillman 1991).
CAF-1 p60 Antibody (SS 24 1-68) - Western blot of CAF-1 p60 expression in 1) HeLa and 2) Cos7 whole cell lysates.

CHAF1B / CAF1 Antibody (aa509-C-term) LS-C287916
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Human
IHC, IP, WB
Unconjugated
100µl 0.2mg/ml  $395.00
Detection of Human CAF-1 p60 by Immunohistochemistry. Sample: FFPE section of human small cell lung cancer. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:200 (1 ug/ml).

CHAF1B / CAF1 Antibody (C-Terminus) LS-C80777
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Human
IHC, IHC-P, WB
Unconjugated
100µl 0.5mg/ml  $335.00
CHAF1B antibody CHAF1B(chromatin assembly factor 1, subunit B (p60)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: Jurkat cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CHAF1B / CAF1 Antibody (C-Terminus, HRP) LS-C448131
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Human, Gibbon
IHC, IHC-P, WB
HRP Conjugated
100µl 0.65mg/ml  $355.00
CHAF1B antibody CHAF1B(chromatin assembly factor 1, subunit B (p60)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: Jurkat cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CHAF1B / CAF1 Antibody (C-Terminus, Biotin) LS-C448129
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Human, Gibbon
IHC, IHC-P, WB
Biotin Conjugated
100µl 1mg/ml  $355.00
CHAF1B antibody CHAF1B(chromatin assembly factor 1, subunit B (p60)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: Jurkat cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CHAF1B / CAF1 Antibody (C-Terminus, FITC) LS-C448130
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Human, Gibbon
IHC, IHC-P, WB
FITC Conjugated
100µl 1mg/ml  $355.00
CHAF1B antibody CHAF1B(chromatin assembly factor 1, subunit B (p60)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human kidney.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Lane A: Marker. Lane B: Jurkat cell lysate. Antibody concentration: 5.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CHAF1B / CAF1 Antibody (clone p60-24) LS-C179638
Mouse Monoclonal [clone p60-24] (IgG1) to Human CHAF1B / CAF1
Human
ICC, IP
Unconjugated
100µg 1mg/ml  $335.00
Immunoprecipitation Image

CHAF1B / CAF1 Antibody (clone 2G7) LS-C198536
Mouse Monoclonal [clone 2G7] (IgG2b,k) to Human CHAF1B / CAF1
Human
ELISA, WB
Unconjugated
100µg  $365.00
Detection limit for recombinant GST tagged CHAF1B is 0.1 ng/ml as a capture antibody.

CHAF1B / CAF1 Antibody (clone SS 53) LS-C2627
Mouse Monoclonal [clone SS 53] (IgG1) to Human CHAF1B / CAF1
Human
IP, WB
Unconjugated
100µl  $335.00
CAF-1 p60 (SS53) Antibody - Western Blot of: A. RKO cells with p150 ab, PCNA ab, CHAF1B Antibody CAF-1 p60 antibody B. p150 RNAi knockdown

CHAF1B / CAF1 Antibody (aa71-120) LS-C119007
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of lysates from Jurkat cells, using CAF1B Antibody. The lane on the right is blocked with the synthesized peptide.

CHAF1B / CAF1 Antibody (aa44-93) LS-C200195
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Mouse, Rat, Human
Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of the lysates from RAW264.7cells using CAF1B antibody.

CHAF1B / CAF1 Antibody (aa509-C-term) LS-C286473
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Human
IHC
Unconjugated
100µl  $415.00
Detection of Human CAF-1 p60 by Immunohistochemistry. Sample: FFPE section of human seminoma. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:250.
Detection of Human CAF-1 p60 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CAF-1 p60 used at a dilution of 1:250.

CHAF1B / CAF1 Antibody (aa1-199) LS-C373672
Rabbit Polyclonal to Mouse CHAF1B / CAF1
Mouse
WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $265.00; 200µl 0.5mg/ml  $305.00; 1ml 0.5mg/ml  $545.00
Western blot of CHAF1B / CAF1 antibody.

CHAF1B / CAF1 Antibody LS-C749087
Rabbit Polyclonal (IgG) to Human CHAF1B / CAF1
Rat, Human
WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00

CHAF1B / CAF1 Antibody (N-Terminus) LS-C111068
Rabbit Polyclonal to Human CHAF1B / CAF1
Xenopus, Chicken, Rat, Pig, Horse, Rabbit, Mouse, Dog, Guinea pig, Bat, Human, Monkey
WB
Unconjugated
100µl 0.5mg/ml  $365.00
CHAF1B antibody Western blot of Fetal Liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Showing 1-25 of 36 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).