CDK5

cyclin-dependent kinase 5

Proline-directed serine/threonine-protein kinase essential for neuronal cell cycle arrest and differentiation and may be involved in apoptotic cell death in neuronal diseases by triggering abortive cell cycle re-entry. Interacts with D1 and D3-type G1 cyclins. Phosphorylates SRC, NOS3, VIM/vimentin, p35/CDK5R1, MEF2A, SIPA1L1, SH3GLB1, PXN, PAK1, MCAM/MUC18, SEPT5, SYN1, DNM1, AMPH, SYNJ1, CDK16, RAC1, RHOA, CDC42, TONEBP/NFAT5, MAPT/TAU, MAP1B, histone H1, p53/TP53, HDAC1, APEX1, PTK2/FAK1, huntingtin/HTT, ATM, MAP2, NEFH and NEFM. Regulates several neuronal development and physiological processes including neuronal survival, migration and differentiation, axonal and neurite growth, synaptogenesis, oligodendrocyte differentiation, synaptic plasticity and neurotransmission, by phosphorylating key proteins. Activated by interaction with CDK5R1 (p35) and CDK5R2 (p39), especially in post-mitotic neurons, and promotes CDK5R1 (p35) expression in an autostimulation loop. Phosphorylates many downstream substrates such as Rho and Ras family small GTPases (e.g. PAK1, RAC1, RHOA, CDC42) or microtubule-binding proteins (e.g. MAPT/TAU, MAP2, MAP1B), and modulates actin dynamics to regulate neurite growth and/or spine morphogenesis. Phosphorylates also exocytosis associated proteins such as MCAM/MUC18, SEPT5, SYN1, and CDK16/PCTAIRE1 as well as endocytosis associated proteins such as DNM1, AMPH and SYNJ1 at synaptic terminals. In the mature central nervous system (CNS), regulates neurotransmitter movements by phosphorylating substrates associated with neurotransmitter release and synapse plasticity; synaptic vesicle exocytosis, vesicles fusion with the presynaptic membrane, and endocytosis. Promotes cell survival by activating anti-apoptotic proteins BCL2 and STAT3, and negatively regulating of JNK3/MAPK10 activity. Phosphorylation of p53/TP53 in response to genotoxic and oxidative stresses enhances its stabilization by preventing ubiquitin ligase-mediated proteasomal degradation, and induces transactivation of p53/TP53 target genes, thus regulating apoptosis. Phosphorylation of p35/CDK5R1 enhances its stabilization by preventing calpain-mediated proteolysis producing p25/CDK5R1 and avoiding ubiquitin ligase-mediated proteasomal degradation. During aberrant cell-cycle activity and DNA damage, p25/CDK5 activity elicits cell-cycle activity and double-strand DNA breaks that precedes neuronal death by deregulating HDAC1. DNA damage triggered phosphorylation of huntingtin/HTT in nuclei of neurons protects neurons against polyglutamine expansion as well as DNA damage mediated toxicity. Phosphorylation of PXN reduces its interaction with PTK2/FAK1 in matrix-cell focal adhesions (MCFA) during oligodendrocytes (OLs) differentiation. Negative regulator of Wnt/beta-catenin signaling pathway. Activator of the GAIT (IFN-gamma-activated inhibitor of translation) pathway, which suppresses expression of a post-transcriptional regulon of proinflammatory genes in myeloid cells; phosphorylates the linker domain of glutamyl-prolyl tRNA synthetase (EPRS) in a IFN-gamma-dependent manner, the initial event in assembly of the GAIT complex. Phosphorylation of SH3GLB1 is required for autophagy induction in starved neurons. Phosphorylation of TONEBP/NFAT5 in response to osmotic stress mediates its rapid nuclear localization. MEF2 is inactivated by phosphorylation in nucleus in response to neurotoxin, thus leading to neuronal apoptosis. APEX1 AP-endodeoxyribonuclease is repressed by phosphorylation, resulting in accumulation of DNA damage and contributing to neuronal death. NOS3 phosphorylation down regulates NOS3-derived nitrite (NO) levels. SRC phosphorylation mediates its ubiquitin-dependent degradation and thus leads to cytoskeletal reorganization. May regulate endothelial cell migration and angiogenesis via the modulation of lamellipodia formation. Involved in dendritic spine morphogenesis by mediating the EFNA1-EPHA4 signaling. The complex p35/CDK5 participates in the regulation of the circadian clock by modulating the function of CLOCK protein: phosphorylates CLOCK at 'Thr-451' and 'Thr-461' and regulates the transcriptional activity of the CLOCK-ARNTL/BMAL1 heterodimer in association with altered stability and subcellular distribution.

☰ Filters

Products

Proteins (13)

Type

Over-Expression Lysate (4)

Recombinant (9)

Target

CDK5 (13)

Publications

No (13)

Tag

6His, N-terminus (1)

GST (2)

GST, N-terminus (2)

His (1)

His-S (1)

His-SUMO (1)

Myc-DDK (Flag) (5)

Species

Human (11)

Source

293T Cells (2)

E. coli (4)

HEK 293 Cells (3)

Insect Cells (1)

Wheat Germ Extract (3)

Purification

Purified (5)

Low Endotoxin Level

Low endotoxin level (1)

Select

Human CDK5 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G65224

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

33.1 kDa

Price:

20 µg/$1,107

Select

Human CDK5 Protein (Recombinant GST, N-terminus) (aa195-292) - LS-G58078

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

Select

Human CDK5 Protein (Recombinant His-SUMO) (Full Length) - LS-G20592

Source:

E. coli

Tag:

His-SUMO

Molecular Weight:

49.28 kD

Price:

1 mg/$1,355; 100 µg/$420; 20 µg/$323

Select

Human CDK5 Protein (Recombinant 6His, N-terminus) (Gln2-Pro292) - LS-G26657

Source:

E. coli

Tag:

6His, N-terminus

Molecular Weight:

33 kDa

Price:

10 µg/$265; 50 µg/$315; 100 µg/$365; 250 µg/$518

Select

Human CDK5 Protein (Recombinant GST) (Full Length) - LS-G27868

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

57.75 kDa

Price:

10 µg/$479; 25 µg/$670

Select

Human CDK5 Protein (Recombinant GST) (Full Length) - LS-G27869

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

57.75 kDa

Price:

10 µg/$479; 25 µg/$670

Select

CDK5 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G100420

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

33.1 kDa

Price:

100 µg/$494

Select

CDK5 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G127982

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

29.4 kDa

Price:

100 µg/$494

Select

Human CDK5 Protein (Recombinant GST, N-terminus) (Met1-Pro292) - LS-G137945

Source:

Insect Cells

Tag:

GST, N-terminus

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

59.6 kDa

Price:

50 µg/$443

Select

Human CDK5 Protein (Recombinant His) - LS-G2055

Source:

E. coli

Tag:

His

Price:

100 µg/$361; 500 µg/$739; 20 µg/$261

Select

Human CDK5 Protein (Recombinant His-S) (aa92-273) - LS-G12735

Source:

E. coli

Tag:

His-S

Molecular Weight:

26.1 kDa

Price:

10 µg/$267; 50 µg/$352; 100 µg/$562; 200 µg/$702; 1 mg/$1,723; 500 µg/$1,298; 5 mg/$2,673; 2 mg/$1,900

Select

Human CDK5 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G75121

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

33.1 kDa

Price:

20 µg/$150

Select

Human CDK5 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G75122

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

29.4 kDa

Price:

20 µg/$150

Viewing 1-13 of 13 product results

(0) Selected Add Selected To Cart Compare Selected Email Selected Clear Selected Top Of Search

---

Filtered By:

Products: Proteins

Clear Filters

If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.