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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CDC73 / Parafibromin

cell division cycle 73

Tumor suppressor probably involved in transcriptional and post-transcriptional control pathways. May be involved in cell cycle progression through the regulation of cyclin D1/PRAD1 expression. Component of the PAF1 complex (PAF1C) which has multiple functions during transcription by RNA polymerase II and is implicated in regulation of development and maintenance of embryonic stem cell pluripotency. PAF1C associates with RNA polymerase II through interaction with POLR2A CTD non-phosphorylated and 'Ser-2'- and 'Ser-5'-phosphorylated forms and is involved in transcriptional elongation, acting both indepentently and synergistically with TCEA1 and in cooperation with the DSIF complex and HTATSF1. PAF1C is required for transcription of Hox and Wnt target genes. PAF1C is involved in hematopoiesis and stimulates transcriptional activity of KMT2A/MLL1; it promotes leukemogenesis through association with KMT2A/MLL1-rearranged oncoproteins, such as KMT2A/MLL1-MLLT3/AF9 and KMT2A/MLL1-MLLT1/ENL. PAF1C is involved in histone modifications such as ubiquitination of histone H2B and methylation on histone H3 'Lys-4' (H3K4me3). PAF1C recruits the RNF20/40 E3 ubiquitin-protein ligase complex and the E2 enzyme UBE2A or UBE2B to chromatin which mediate monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1); UB2A/B-mediated H2B ubiquitination is proposed to be coupled to transcription. PAF1C is involved in mRNA 3' end formation probably through association with cleavage and poly(A) factors. In case of infection by influenza A strain H3N2, PAF1C associates with viral NS1 protein, thereby regulating gene transcription. Connects PAF1C with the cleavage and polyadenylation specificity factor (CPSF) complex and the cleavage stimulation factor (CSTF) complex, and with Wnt signaling. Involved in polyadenylation of mRNA precursors.

Gene Name: cell division cycle 73
Synonyms: CDC73, C1orf28, Hyperparathyroidism 2 protein, HPTJT, HRPT2, Parafibromin, HYX
Target Sequences: NM_024529 NP_078805.3 Q6P1J9

Filtered By: None
Target
CDC73 / Parafibromin (29)
Reactivity
Human (29)
Mouse (12)
Rat (10)
Bovine (9)
Chicken (8)
Chimpanzee (6)
Dog (9)
Guinea pig (1)
Horse (9)
Monkey (4)
Pig (1)
Rabbit (9)
Xenopus (4)
Zebrafish (8)
Application
IHC (12)
IHC-Fr (2)
IHC-P (3)
WB (23)
Flo (9)
ELISA (13)
IF (11)
IP (2)
Peptide-ELISA (1)
Host
rabbit (27)
mouse (2)
Isotype
IgG (17)
Clonality
monoclonal mc (2)
polyclonal pc (27)
Clone
BSB-50 (2)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (4)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (1)
Ready-to-Use (1)
Unconjugated (14)
Epitope
aa132-161 (8)
aa385-434 (4)
aa394-443 (4)
aa119-520 (1)
aa127-161 (1)
aa250-300 (1)
Publications
No (29)
Showing 1-25 of 29 results

CDC73 / Parafibromin Antibody (clone BSB-50) LS-C348558
Mouse Monoclonal [clone BSB-50] (IgG) to Human CDC73 / Parafibromin
Mouse, Human
IHC IHC-Fr IHC-P
Unconjugated
100µl  $245.00; 500µl 100µg/ml  $455.00; 1000µl 100µg/ml  $785.00
IHC of Parafibromin on FFPE Parathyroid Carcinoma Tissue.
IHC of Parafibromin on FFPE Parathyroid Carcinoma Tissue.

CDC73 / Parafibromin Antibody (aa132-161) LS-C168598
Rabbit Polyclonal to Human CDC73 / Parafibromin
Human
Flo IF IHC IHC-P WB
Unconjugated
400µl  $305.00
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CDC73 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of CDC73 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).
Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with CDC73 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Confocal immunofluorescence of CDC73 Antibody with HeLa cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).

CDC73 / Parafibromin Antibody (aa132-161) LS-C320244
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
ELISA Flo IF IHC WB
Unconjugated
200µl  $530.00

CDC73 / Parafibromin Antibody (aa132-161, Biotin) LS-C208311
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
ELISA Flo IF IHC WB
Biotin Conjugated
200µl  $680.00

CDC73 / Parafibromin Antibody (aa132-161, FITC) LS-C208312
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
ELISA Flo IF IHC WB
FITC Conjugated
200µl  $680.00

CDC73 / Parafibromin Antibody (aa132-161, APC) LS-C208310
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
ELISA Flo IF IHC WB
APC Conjugated
200µl  $680.00

CDC73 / Parafibromin Antibody (aa132-161, AP) LS-C208309
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
ELISA Flo IF IHC WB
AP Conjugated
200µl  $680.00

CDC73 / Parafibromin Antibody (aa132-161, Azide-free, HRP) LS-C208313
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
ELISA Flo IF IHC WB
HRP Conjugated, Azide-free
200µl  $680.00

CDC73 / Parafibromin Antibody (aa132-161, PE) LS-C208314
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
ELISA Flo IF IHC WB
PE Conjugated
200µl  $680.00

CDC73 / Parafibromin Antibody LS-C331037
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Mouse, Human
Flo IF IHC WB
Unconjugated
50µl  $305.00
Western blot analysis of extracts of mouse testis, using CDC73 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
Western blot analysis of extracts of mouse testis, using CDC73 antibody. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.

CDC73 / Parafibromin Antibody LS-C666218
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
ELISA IF IHC IP WB
Unconjugated
100µg  $335.00

CDC73 / Parafibromin Antibody (clone BSB-50, Ready-to-Use) LS-C348557
Mouse Monoclonal [clone BSB-50] (IgG) to Human CDC73 / Parafibromin
Human
IHC IHC-Fr IHC-P
Unconjugated, Ready-to-Use
3000µl  $215.00; 7000µl  $265.00; 15000µl  $375.00
IHC of Parafibromin on FFPE Parathyroid Carcinoma Tissue. This image was taken for the unmodified form of this product. Other forms have not been tested.
IHC of Parafibromin on FFPE Parathyroid Carcinoma Tissue. This image was taken for the unmodified form of this product. Other forms have not been tested.

CDC73 / Parafibromin Antibody LS-C677456
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
ELISA IF
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunofluorescent analysis of Hela cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
Immunofluorescent analysis of Hela cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

CDC73 / Parafibromin Antibody (HRP) LS-C677460
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
ELISA
HRP Conjugated
50µg  $285.00; 100µg  $385.00

CDC73 / Parafibromin Antibody (Biotin) LS-C677459
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
ELISA
Biotin Conjugated
50µg  $285.00; 100µg  $385.00

CDC73 / Parafibromin Antibody (FITC) LS-C677463
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
ELISA
FITC Conjugated
50µg  $285.00; 100µg  $385.00

CDC73 / Parafibromin Antibody LS-C413735
Rabbit Polyclonal to Human CDC73 / Parafibromin
Mouse, Rat, Human
ELISA WB
Unconjugated
100µg 1mg/ml  $345.00
The Parafibromin Antibody is used in Western blot to detect Parafibromin in HL-60 cell lysate.
The Parafibromin Antibody is used in Western blot to detect Parafibromin in HL-60 cell lysate.

CDC73 / Parafibromin Antibody (aa127-161) LS-C392840
Rabbit Polyclonal to Human CDC73 / Parafibromin
Human
Peptide-ELISA WB
Unconjugated
50µl  $205.00
Anti-CDC73 Antibody (Center) at 1:2000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
Anti-CDC73 Antibody (Center) at 1:2000 dilution + 293T/17 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 61 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

CDC73 / Parafibromin Antibody (aa250-300) LS-C287095
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Pig, Horse, Human
IP WB
Unconjugated
100µl 1mg/ml  $395.00
Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human Parafibromin by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Parafibromin, was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of Human and Mouse Para fibromin by Western Blot. Samples: Whole cell lysate (50 ug) from mouse TCMK-1 and human 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Detection of Human Parafibromin by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified rabbit anti-Parafibromin antibody used for IP at 6 ug/mg lysate. For blotting immunoprecipitated Parafibromin, was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

CDC73 / Parafibromin Antibody LS-C490207
Rabbit Polyclonal (IgG) to Human CDC73 / Parafibromin
Human
WB
Unconjugated
100µg  $365.00
Western blot Image
Western blot Image

CDC73 / Parafibromin Antibody (aa394-443) LS-C146451
Rabbit Polyclonal to Human CDC73 / Parafibromin
Xenopus, Chicken, Zebrafish, Horse, Rat, Bovine, Rabbit, Mouse, Dog, Human
WB
Unconjugated
50µg  $365.00
CDC73 / Parafibromin antibody Western Blot of Human HeLa Cell. Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDC73 / Parafibromin antibody Western Blot of Human HeLa Cell. Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CDC73 / Parafibromin Antibody (aa394-443, HRP) LS-C471240
Rabbit Polyclonal to Human CDC73 / Parafibromin
Xenopus, Chicken, Zebrafish, Rat, Horse, Bovine, Rabbit, Mouse, Dog, Human, Chimpanzee
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
CDC73 / Parafibromin antibody Western Blot of Human HeLa Cell. Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDC73 / Parafibromin antibody Western Blot of Human HeLa Cell. Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CDC73 / Parafibromin Antibody (aa394-443, FITC) LS-C471239
Rabbit Polyclonal to Human CDC73 / Parafibromin
Xenopus, Chicken, Zebrafish, Rat, Horse, Bovine, Rabbit, Mouse, Dog, Human, Chimpanzee
WB
FITC Conjugated
100µl 1mg/ml  $385.00
CDC73 / Parafibromin antibody Western Blot of Human HeLa Cell. Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDC73 / Parafibromin antibody Western Blot of Human HeLa Cell. Antibody dilution: 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CDC73 / Parafibromin Antibody (aa385-434) LS-C151536
Rabbit Polyclonal to Human CDC73 / Parafibromin
Chicken, Zebrafish, Horse, Rabbit, Mouse, Dog, Bovine, Rat, Human, Monkey
WB
Unconjugated
50µg  $365.00
CDC73 / Parafibromin antibody Western Blot of MDA-MB-435S.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
CDC73 / Parafibromin antibody Western Blot of MDA-MB-435S.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

CDC73 / Parafibromin Antibody (aa119-520) LS-C407745
Rabbit Polyclonal to Human CDC73 / Parafibromin
Human
WB
Unconjugated
100µg  $325.00
HRPT2 antibody Western blot. All lanes: Anti HRPT2 at 0.5 ug/ml. Lane 1: Human Placenta Tissue Lysate at 50 ug. Lane 2: HEPG2 Whole Cell Lysate at 40 ug. Lane 3: 293T Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 61 kD. Observed band size: 61 kD.
HRPT2 antibody Western blot. All lanes: Anti HRPT2 at 0.5 ug/ml. Lane 1: Human Placenta Tissue Lysate at 50 ug. Lane 2: HEPG2 Whole Cell Lysate at 40 ug. Lane 3: 293T Whole Cell Lysate at 40 ug. Lane 4: HELA Whole Cell Lysate at 40 ug. Predicted band size: 61 kD. Observed band size: 61 kD.
Showing 1-25 of 29 results

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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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