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CDC25C

cell division cycle 25C

CDC25C, a CDC25 dual specificity protein phosphatase, has been shown to affect the regulation of cell cycle control, and specifically the G2/M progression, through interaction with cdc/cyclin kinases and proliferating cell nuclear antigen (PCNA). Unlike CDC25A and CDC25B, CDC25C does not seem to have oncogenic properties. CDC25C null mutant mice are viable, fertile, and do not display obvious abnomalities. CDC25C expression is regulated by chk1, 14-3-3 protein, p53 and BRCA1, among other proteins. CDC25C is located on chromosome 5p31 near a region that is deleted in some hematologic disorders.

Gene Name: cell division cycle 25C
Family/Subfamily: Protein Phosphatase , CDC25
Synonyms: CDC25C, CDC25, CDC25C phosphatase, CDC25Hu3, Cell division cycle 25C, Phosphotyrosine phosphatase, PPP1R60, M-phase inducer phosphatase 3, Mitosis inducer CDC25
Target Sequences: NM_001790 NP_001781.2 P30307

Publications (2)

1
XJW20, a novel oxoindole derivative, induces G2/M arrest and apoptosis selectively in K562 leukemia cell line. Ye Z, Chen Z, Chen W, Xie J, Yang H, Lou Y, Yu Y. Chemico-biological interactions. 2010 183:133-41. (WB) [PubMed:19883635]
2
Ochratoxin A induces G(2) phase arrest in human gastric epithelium GES-1 cells in vitro. Cui J, Xing L, Li Z, Wu S, Wang J, Liu J, Wang J, Yan X, Zhang X. Toxicology letters. 2010 193:152-8. (WB) [PubMed:20060447]

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Proteins (10)
Over-Expression Lysate (6)
Recombinant (4)
CDC25C (10)
No (10)
6His, N-terminus (1)
GST (1)
GST, N-terminus (1)
Myc-DDK (Flag) (7)
Human (7)
293T Cells (3)
E. coli (1)
HEK 293 Cells (4)
Wheat Germ Extract (2)
Purified (2)
CDC25C Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
6His, N-terminus
1 mg/$1,355; 100 µg/$420; 20 µg/$323
CDC25C Protein - Purified recombinant protein CDC25C was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
53.2 kDa
20 µg/$1,107
CDC25C Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
CDC25C Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
53.2 kDa
20 µg/$150
CDC25C Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
45.4 kDa
20 µg/$150
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293T Cells
Myc-DDK (Flag)
45.4 kDa
20 µg/$150
CDC25C Protein - 12.5% SDS-PAGE of human CDC25C stained with Coomassie Blue
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Wheat Germ Extract
GST
79.7 kDa
10 µg/$479; 25 µg/$670
CDC25C Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
45.4 kDa
100 µg/$494
CDC25C Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
45.4 kDa
100 µg/$494
CDC25C Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
53.2 kDa
100 µg/$494
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).