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CD20

membrane-spanning 4-domains, subfamily A, member 1

CD20 is a member of the membrane-spanning 4A gene family. Members of this nascent protein family are characterized by common structural features and similar intron/exon splice boundaries and display unique expression patterns among hematopoietic cells and nonlymphoid tissues. This gene encodes a B-lymphocyte surface molecule which plays a role in the development and differentiation of B-cells into plasma cells. This family member is localized to 11q12, among a cluster of family members. Alternative splicing of this gene results in two transcript variants which encode the same protein.

Gene Name: membrane-spanning 4-domains, subfamily A, member 1
Synonyms: MS4A1, B-lymphocyte antigen CD20, B1, Bp35, CD20, CD20 antigen, CD20 receptor, LEU-16, CVID5
Target Sequences: NM_021950 NP_690605.1 P11836

Publications (4)

1
BDNF and its receptors in human myasthenic thymus: implications for cell fate in thymic pathology. Berzi A, Ayata CK, Cavalcante P, Falcone C, Candiago E, Motta T, Bernasconi P, Hohlfeld R, Mantegazza R, Meinl E, Farina C. Journal of neuroimmunology. 2008 197:128-39. (ICC) [PubMed:18555538]
2
Humanized Mice Exhibit Exacerbated Abscess Formation and Osteolysis During the Establishment of Implant-Associated Staphylococcus aureus Osteomyelitis. Gowrishankar Muthukrishnan, Alexandra Wallimann, Javier Rangel-Moreno, Karen L de Mesy Bentley, Maria Hildebrand, Karen Mys, H Mark Kenney, Eric T Sumrall, John L Daiss, Stephan Zeiter, R Geoff Richards, Edward M Schwarz, T Fintan Moriarty. Frontiers in immunology. 2021 April;12:651515. [Full Text Article] [PubMed:33815412] [PMC:PMC8012494] Related Antibodies: LS-B2605.
3
PD-1 blockade and vaccination provide therapeutic benefit against SIV by inducing broad and functional CD8+ T cells in lymphoid tissue. Sheikh Abdul Rahman, Bhrugu Yagnik, Alexander P Bally, Kristen N Morrow, Shelly Wang, Thomas H Vanderford, Gordon J Freeman, Rafi Ahmed, Rama Rao Amara. Science immunology. 2021 September;6:eabh3034. [Full Text Article] [PubMed:34516743] [PMC:PMC8500359] Related Antibodies: LS-B11144.
4
Tumor-initiating properties of breast cancer and melanoma cells in vivo are not invariably reflected by spheroid formation in vitro, but can be increased by long-term culturing as adherent monolayers. Kuch V, Schreiber C, Thiele W, Umansky V, Sleeman JP. International journal of cancer. Journal international du cancer. 2013 Feb;132:E94-105. [Full Text Article] [PubMed:22907275] Related Antibodies: LS-C13105.
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Products
Proteins (16)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (4)
Recombinant (11)
CD20 (16)
SDS-PAGE (2)
No (16)
6His, N-terminus (3)
6His-SUMO, N-terminus (1)
GST (1)
GST, N-terminus (1)
His, N-Terminal (1)
His,N-terminus (1)
His-GST, N-terminus (1)
Myc-DDK (Flag) (5)
TRX (1)
TRX, N-terminus (1)
Human (11)
Mouse (3)
293T Cells (2)
E. coli (4)
HEK 293 Cells (6)
Wheat Germ Extract (2)
Yeast (2)
Purified (5)
Low endotoxin level (2)
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Yeast
6His, N-terminus
22.3 kD
100 µg/$499; 20 µg/$364; 1 mg/$1,683
CD20 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Yeast
6His, N-terminus
100 µg/$446; 20 µg/$336; 1 mg/$1,460
CD20 Protein - Recombinant Membrane Spanning 4 Domains Subfamily A, Member 1 By SDS-PAGE
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E. coli
His-GST, N-terminus
59.0 kDa
10 µg/$278; 50 µg/$422; 1 mg/$1,934; 200 µg/$842; 100 µg/$675
CD20 Protein - (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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E. coli
6His-SUMO, N-terminus
1 mg/$1,679; 100 µg/$500; 20 µg/$364
CD20 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
32.9 kDa
100 µg/$494
Select
HEK 293 Cells
His,N-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
12.1 kDa
20 µg/$603
CD20 Protein
Select
HEK 293 Cells
His, N-Terminal
SDS-PAGE
Less than 1.0 EU/µg protein (determined by LAL method).
The recombinant human CD20 comprises 105 amino acids and has a predicted molecular mass of 12.1 kDa. The apparent molecular mass of the protein is approximately 23 kDa in SDS-PAGE under reducing conditions due to glycosylation.
20 µg/$317
CD20 Protein
Select
E. coli
TRX, N-terminus
SDS-PAGE
The recombinant human MS4A1 consists 218 amino acids and predicts a molecular mass of 23.9 kDa.
100 µg/$480
CD20 Protein - 12.5% SDS-PAGE of human MS4A1 stained with Coomassie Blue
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Wheat Germ Extract
GST
58.41 kDa
10 µg/$479; 25 µg/$670
CD20 Protein - Purified recombinant protein MS4A1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
32.9 kDa
20 µg/$1,107
CD20 Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
2 µg/$439
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293T Cells
Myc-DDK (Flag)
32.9 kDa
20 µg/$150
CD20 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
32.9 kDa
20 µg/$150
Select
HEK 293 Cells
6His, N-terminus
33 kDa
200 µl/$283
CD20 Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
32.9 kDa
100 µg/$494
Select
E. coli
TRX
23.9 kDa
100 µg/$966
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).