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CA2 / Carbonic Anhydrase II

carbonic anhydrase II

Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.

Gene Name: carbonic anhydrase II
Synonyms: CA2, CA-II, CAII, CAC, Car2, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II, Carbonic dehydratase, Carbonic anhydrase 2, Carbonic anhydrase B
Target Sequences: NM_000067 NP_000058.1 P00918

Publications (6)

1
Carbonic anhydrase II binds to and enhances activity of the Na+/H+ exchanger. Li X, Alvarez B, Casey JR, Reithmeier RA, Fliegel L. The Journal of biological chemistry. 2002 277:36085-91. [PubMed:12138085]
2
Inhibition of cell growth and nuclear factor-kappaB activity in pancreatic cancer cell lines by a tylophorine analogue, DCB-3503. Shiah HS, Gao W, Baker DC, Cheng YC. Molecular cancer therapeutics. 2006 5:2484-93. [PubMed:17041092]
3
Catalysis and pH control by membrane-associated carbonic anhydrase IX in MDA-MB-231 breast cancer cells. Li Y, Tu C, Wang H, Silverman DN, Frost SC. The Journal of biological chemistry. 2011 286:15789-96. [PubMed:21454639] [PMC:PMC3091188]
4
Role of zinc in catalytic activity of carbonic anhydrase IX. Tu C, Foster L, Alvarado A, McKenna R, Silverman DN, Frost SC. Archives of biochemistry and biophysics. 2012 521:90-4. (WB; Human) [PubMed:22465027] [PMC:PMC3345035]
5
Loss of carbonic anhydrase XII function in individuals with elevated sweat chloride concentration and pulmonary airway disease. Melissa Lee, Briana Vecchio-Pagán, Neeraj Sharma, Abdul Waheed, Xiaopeng Li, Karen S Raraigh, Sarah Robbins, Sangwoo T Han, Arianna L Franca, Matthew J Pellicore, Taylor A Evans, Kristin M Arcara, Hien Nguyen, Shan Luan, Deborah Belchis, Jozef Hertecant, Joseph Zabner, William S Sly, Garry R Cutting. Human molecular genetics. 2016 May;25:1923-1933. [Full Text Article] [PubMed:26911677] [PMC:PMC5062583]
6
Quantitative Secretome Analysis Reveals Clinical Values of Carbonic Anhydrase II in Hepatocellular Carcinoma. Xiaohua Xing, Hui Yuan, Hongzhi Liu, Xionghong Tan, Bixing Zhao, Yingchao Wang, Jiahe Ouyang, Minjie Lin, Xiaolong Liu, Aimin Huang. Genomics, proteomics & bioinformatics. 2021 Feb;;19:94-107. [Full Text Article] [PubMed:33662630] [PMC:PMC8498920]
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Antibodies (145)
Primary (145)
CA2 / Carbonic Anhydrase II (145)
Human (95)
Mouse (51)
Rat (24)
Bat (3)
Bovine (10)
Chicken (8)
Chimpanzee (6)
Dog (14)
Gibbon (6)
Horse (6)
Monkey (25)
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IHC (24)
IHC-P (10)
WB (139)
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DB (2)
ELISA (32)
ICC (17)
ID (2)
IF (3)
IP (20)
Peptide-ELISA (2)
rabbit (129)
mouse (10)
guinea pig (3)
rat (1)
sheep (2)
Product Group
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IgG (36)
IgG2a (1)
monoclonal mc (11)
polyclonal pc (134)
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Biotin Conjugated (21)
Cy3 Conjugated (10)
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aa1-260 (37)
aa5-258 (8)
aa130-179 (4)
aa73-122 (4)
aa2-260 (2)
Asn229 (1)
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C-terminal (1)
aa180-229 (1)
aa54-83 (1)
No (145)
CA2 / Carbonic Anhydrase II Antibody - Anti-Carbonic Anhydrase II antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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CA2 / Carbonic Anhydrase II Rabbit anti-Bovine Polyclonal Antibody
Bovine, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µl/$460
CA2 / Carbonic Anhydrase II Antibody - Anti-Carbonic Anhydrase II antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (HRP) Antibody
Human
ELISA, IHC, IHC-P, WB
HRP Conjugated
50 µg/$635
CA2 / Carbonic Anhydrase II Antibody - Anti-Carbonic Anhydrase II antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$460
CA2 / Carbonic Anhydrase II Antibody - Fluorescent confocal image of HeLa cell stained with CA2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CA2 immunoreactivity is localized to Cytoplasm significantly.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (aa54-83) Antibody
Mouse, Human
IF, WB
Unconjugated
400 µl/$393
CA2 / Carbonic Anhydrase II Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (aa2-260) Antibody
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$460
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CA2 / Carbonic Anhydrase II Rat anti-Human Monoclonal (aa2-260) Antibody
Human
ELISA, WB
Unconjugated
100 µg/$896
CA2 / Carbonic Anhydrase II Antibody - Western blot of recombinant CA2 / Carbonic Anhydrase II.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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CA2 / Carbonic Anhydrase II Rabbit anti-Chicken Polyclonal (aa1-260) Antibody
Chicken, Human
WB
Unconjugated
100 µl/$306; 200 µl/$347; 500 µl/$484; 1 ml/$553; 2 ml/$737; 5 ml/$1,206
CA2 / Carbonic Anhydrase II Antibody - CA2 was detected in paraffin-embedded sections of human gastric cancer tissues using rabbit anti- CA2 Antigen Affinity purified polyclonal antibody
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Human
Flo, ICC, IHC, IHC-P, WB
Unconjugated
10 µg/$318; 100 µg/$470
CA2 / Carbonic Anhydrase II Antibody - Western Blot of Rabbit anti-Carbonic Anhydrase II Antibody Biotin Conjugated. Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Biotin Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (Lyophilized) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated, Lyophilized
100 µg/$552
CA2 / Carbonic Anhydrase II Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$460
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CA2 / Carbonic Anhydrase II Rabbit anti-Mouse Polyclonal Antibody
Mouse
ELISA, IHC, IHC-P, IP, WB
Unconjugated
100 µl/$412
CA2 / Carbonic Anhydrase II Antibody - Western blot analysis of extracts of various cell lines, using CA2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
CA2 / Carbonic Anhydrase II Antibody - 1:100 staining human liver carcinoma tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
2 ml/$1,225
CA2 / Carbonic Anhydrase II Antibody - Western blot of CA II antibody
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (C-terminal) Antibody
Rat, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
CA2 / Carbonic Anhydrase II Antibody - IHC testing of FFPE human gastric cancer tissue with CA2 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
Flo, IHC-P, WB
Unconjugated
100 µg/$575
CA2 / Carbonic Anhydrase II Antibody - Dot Blot of Rabbit Anti-Carbonic Anhydrase II Peroxidase Conjugated Antibody. Lane 1: 100ng. Lane 2: 33.3ng. Lane 3: 11.1ng. Lane 4: 3.7ng. Lane 5: 1.23ng. Secondary Antibody: Anti-Carbonic Anhydrase II HRP 1µg/mL.
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CA2 / Carbonic Anhydrase II Rabbit anti-Bovine Polyclonal (Lyophilized) (HRP) Antibody
Bovine
DB, ELISA, IHC, WB
HRP Conjugated, Lyophilized
100 µg/$552
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman’s capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3’3-diamino-benzidine substrate (DAB) or WARP RED.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (Liquid) Antibody
Human
ELISA, IHC, WB
Unconjugated, Liquid
25 µl/$304
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman’s capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3’3-diamino-benzidine substrate (DAB) or WARP RED.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (Lyophilized) Antibody
Human
ELISA, IHC, WB
Unconjugated, Lyophilized
100 µg/$567
CA2 / Carbonic Anhydrase II Antibody - ELISA results of purified Rabbit Carbonic Anhydrase II Antibody tested against Carbonic Anhydrase II (erythrocytes). (Green line) The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.
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CA2 / Carbonic Anhydrase II Rabbit anti-Bovine Polyclonal (Liquid) (HRP) Antibody
Bovine
DB, ELISA, IHC, WB
HRP Conjugated, Liquid
25 µl/$304
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry of Rabbit anti Carbonic Anhydrase II antibody Tissue: intestine Staining: diffuse positivity of enzyma Carbonic Anidrase II in brush border of villus
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CA2 / Carbonic Anhydrase II Rabbit anti-Bovine Polyclonal (Lyophilized) Antibody
Bovine
ELISA, IHC, WB
Unconjugated, Lyophilized
100 µg/$567
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CA2 / Carbonic Anhydrase II Sheep anti-Human Polyclonal Antibody
Human
ID, IF, WB
Unconjugated
1 ml/$448
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry analysis of CA II antibody in paraffin-embedded human lung carcinoma tissue.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (aa180-229) Antibody
Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
50 µl/$334; 100 µl/$397
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry of rabbit anti Carbonic Anhydrase II antibody Tissue: intestine Staining: diffuse positivity of enzyma Carbonic Anidrase II in brush border of villus
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CA2 / Carbonic Anhydrase II Rabbit anti-Bovine Polyclonal (Liquid) Antibody
Bovine
ELISA, IHC, WB
Unconjugated, Liquid
25 µl/$304
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemical analysis of Carbonic Anhydrase 2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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CA2 / Carbonic Anhydrase II Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Rat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100 µl/$299
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).