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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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CA2 / Carbonic Anhydrase II

carbonic anhydrase II

Essential for bone resorption and osteoclast differentiation (By similarity). Reversible hydration of carbon dioxide. Can hydrate cyanamide to urea. Involved in the regulation of fluid secretion into the anterior chamber of the eye. Contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. Stimulates the chloride-bicarbonate exchange activity of SLC26A6.

Gene Name: carbonic anhydrase II
Synonyms: CA2, CA-II, CAII, CAC, Car2, Carbonate dehydratase II, Carbonic anhydrase C, Carbonic anhydrase II, Carbonic dehydratase, Carbonic anhydrase 2, Carbonic anhydrase B
Target Sequences: NM_000067 NP_000058.1 P00918

Publications (4)

1
Carbonic anhydrase II binds to and enhances activity of the Na+/H+ exchanger. Li X, Alvarez B, Casey JR, Reithmeier RA, Fliegel L. The Journal of biological chemistry. 2002 277:36085-91. [PubMed:12138085]
2
Inhibition of cell growth and nuclear factor-kappaB activity in pancreatic cancer cell lines by a tylophorine analogue, DCB-3503. Shiah HS, Gao W, Baker DC, Cheng YC. Molecular cancer therapeutics. 2006 5:2484-93. [PubMed:17041092]
3
Catalysis and pH control by membrane-associated carbonic anhydrase IX in MDA-MB-231 breast cancer cells. Li Y, Tu C, Wang H, Silverman DN, Frost SC. The Journal of biological chemistry. 2011 286:15789-96. [PubMed:21454639] [PMC:PMC3091188]
4
Role of zinc in catalytic activity of carbonic anhydrase IX. Tu C, Foster L, Alvarado A, McKenna R, Silverman DN, Frost SC. Archives of biochemistry and biophysics. 2012 521:90-4. (WB; Human) [PubMed:22465027] [PMC:PMC3345035]
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Viewing 1-25 of 147 items
CA2 / Carbonic Anhydrase II Antibody - Anti-Carbonic Anhydrase II antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Rabbit Polyclonal to Bovine CA2 / Carbonic Anhydrase II
Bovine, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl   10mg/ml   $425.00
CA2 / Carbonic Anhydrase II Antibody - Anti-Carbonic Anhydrase II antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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Rabbit Polyclonal to Human CA2 / Carbonic Anhydrase II
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg   50µl   1mg/ml   $425.00
CA2 / Carbonic Anhydrase II Antibody - Anti-Carbonic Anhydrase II antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal to Human CA2 / Carbonic Anhydrase II
Human
ELISA, IHC, IHC-P, WB
HRP Conjugated
50µg   10mg/ml   $595.00
CA2 / Carbonic Anhydrase II Antibody - Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE)
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Rabbit Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µg   3mg/ml   $425.00
CA2 / Carbonic Anhydrase II Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
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Rabbit Polyclonal to Human CA2 / Carbonic Anhydrase II
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50µl   $425.00
CA2 / Carbonic Anhydrase II Antibody - Fluorescent confocal image of HeLa cell stained with CA2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CA2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CA2 immunoreactivity is localized to Cytoplasm significantly.
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Rabbit Polyclonal to Human CA2 / Carbonic Anhydrase II
Mouse, Human
IF, WB
Unconjugated
400µl   $325.00
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Rat Monoclonal (IgG2a) to Human CA2 / Carbonic Anhydrase II
Human
ELISA, WB
Unconjugated
100µg   $650.00
CA2 / Carbonic Anhydrase II Antibody - CA2 was detected in paraffin-embedded sections of human gastric cancer tissues using rabbit anti- CA2 Antigen Affinity purified polyclonal antibody
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Rabbit Polyclonal to Human CA2 / Carbonic Anhydrase II
Human
Flo, ICC, IHC, IHC-P, WB
Unconjugated
100µg   $355.00
CA2 / Carbonic Anhydrase II Antibody - Western Blot of Rabbit anti-Carbonic Anhydrase II Antibody Biotin Conjugated. Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Biotin Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: HRP streptavidin secondary antibody at 1:40,000 for 30 min at RT.
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Rabbit Polyclonal to Human CA2 / Carbonic Anhydrase II
Human
ELISA, WB
Biotin Conjugated
100µg   1mg/ml   $425.00
CA2 / Carbonic Anhydrase II Antibody - Western blot of recombinant CA2 / Carbonic Anhydrase II.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal to Chicken CA2 / Carbonic Anhydrase II
Chicken, Human
WB
Unconjugated
20µl   0.5mg/ml   $205.00; 100µl   0.5mg/ml   $255.00; 200µl   0.5mg/ml   $295.00; 1ml   0.5mg/ml   $515.00
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Rabbit Polyclonal to Mouse CA2 / Carbonic Anhydrase II
Mouse
ELISA, IHC, IHC-P, IP, WB
Unconjugated
50µl   $235.00; 100µl   $275.00; 200µl   $305.00
CA2 / Carbonic Anhydrase II Antibody
Select
Rabbit Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
CA2 / Carbonic Anhydrase II Antibody - Western blot analysis of extracts of various cell lines, using CA2 antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking.
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Rabbit Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Mouse, Human
IF, IHC, WB
Unconjugated
50µl   $235.00; 100µl   $315.00; 200µl   $425.00
CA2 / Carbonic Anhydrase II Antibody - ELISA results of purified Rabbit Carbonic Anhydrase II Antibody tested against Carbonic Anhydrase II (erythrocytes). (Green line) The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using TMB-1000 substrate.
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Rabbit Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Cow, Human
DB, ELISA, IHC, WB
HRP Conjugated
25µl   1mg/ml   $235.00
CA2 / Carbonic Anhydrase II Antibody - Western blot of CA II antibody
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Rabbit Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Rat, Human
ELISA, IHC, WB
Unconjugated
50µl   1mg/ml   $235.00; 100µl   1mg/ml   $265.00; 200µl   1mg/ml   $335.00
CA2 / Carbonic Anhydrase II Antibody - IHC testing of FFPE human gastric cancer tissue with CA2 antibody at 1ug/ml. HIER: steam in pH6 citrate buffer and allow to cool prior to staining.
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Rabbit Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Mouse, Rat, Human
Flo, IHC-P, WB
Unconjugated
100µg   $435.00
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman’s capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3’3-diamino-benzidine substrate (DAB) or WARP RED.
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Rabbit Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Human
ELISA, IHC, WB
Unconjugated
25µl   1.1mg/ml   $235.00
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry with anti-carbonic anhydrase II antibody showing carbonic anhydrase II staining in nucleus and cytoplasm of proximal and distal tubules, bowman’s capsule and glomerular podocytes of human kidney (B) and in basal cells of retina of a mouse eye (C). Formalin fixed/paraffin embedded sections were subjected to heat induced epitope retrieval (HIER) at pH 6.2 and then incubated with rabbit anti-carbonic anhydrase II antibody at 4.0 µg/ml for 60 minutes. The reaction was developed using either MACH 1 universal HRP polymer detection (human kidney) or MACH 4 universal AP polymer detection system (mouse eye) and visualized with 3’3-diamino-benzidine substrate (DAB) or WARP RED.
Select
Rabbit Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Human
ELISA, IHC, WB
Unconjugated
100µg   1mg/ml   $425.00
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry of rabbit anti Carbonic Anhydrase II antibody Tissue: intestine Staining: diffuse positivity of enzyma Carbonic Anidrase II in brush border of villus
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Rabbit Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Cow, Human
ELISA, IHC, WB
Unconjugated
25µl   1mg/ml   $235.00
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemistry analysis of CA II antibody in paraffin-embedded human lung carcinoma tissue.
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Rabbit Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
50µl   1mg/ml   $265.00; 100µl   1mg/ml   $325.00
CA2 / Carbonic Anhydrase II Antibody - Immunohistochemical analysis of Carbonic Anhydrase 2 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Rabbit Polyclonal to Human CA2 / Carbonic Anhydrase II
Rat, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $245.00
Select
Sheep Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Human
ID, IF, WB
Unconjugated
1ml   $455.00
Select
Rabbit Polyclonal to Mouse CA2 / Carbonic Anhydrase II
Mouse
IHC, IHC-P
Unconjugated
50µl   $245.00; 100µl   $305.00
CA2 / Carbonic Anhydrase II Antibody - Western Blot of rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugated. Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II.
Select
Rabbit Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Human
ELISA, IHC, WB
HRP Conjugated
25µl   1mg/ml   $235.00
CA2 / Carbonic Anhydrase II Antibody - Western Blot of rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugated. Lane 1: Carbonic Anhydrase II. Load: 50 ng per lane. Primary antibody: Rabbit anti-Carbonic Anhydrase II Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 minutes at RT. Predicted/Observed size: 29 kDa, 29 kDa for Carbonic Anhydrase II.
Select
Rabbit Polyclonal (IgG) to Human CA2 / Carbonic Anhydrase II
Human
ELISA, IHC, WB
HRP Conjugated
100µg   1mg/ml   $425.00

Viewing 1-25 of 147 items


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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).