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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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C-TAK1 / MARK3

MAP/microtubule affinity-regulating kinase 3

MARK3, a MARK type protein kinase, was originally identified as a marker induced with DNA damaging agents. The protein contains a N-terminal kinase domain. It has been shown to bind and phosphorylate CDC25C and to regulate Ras signaling by phosphorylating the MAPK scaffold, KSR1. At least three mRNA transcripts have been reported -- 3.0-, 3.1-, and 3.8-kb. Loss of MARK3 has been associated with carcinogenesis in the pancreas.

Gene Name: MAP/microtubule affinity-regulating kinase 3
Family/Subfamily: Protein Kinase , MARK
Synonyms: MARK3, C-TAK1, ELKL motif kinase 2, EMK2, Etk-1, KP78, PAR1A, Ser/Thr protein kinase PAR-1, Par-1a, CTAK1, EMK-2, Protein kinase STK10
Target Sequences: NM_002376 NP_002367.5 P27448

Filtered by: None
Target
C-TAK1 / MARK3 (62)
Reactivity
Human (62)
Mouse (19)
Rat (9)
Bovine (4)
Chicken (1)
Chimpanzee (3)
Dog (4)
Gibbon (3)
Guinea pig (4)
Horse (4)
Monkey (4)
Rabbit (4)
Xenopus (4)
Zebrafish (1)
Application
IHC (24)
IHC-P (8)
WB (48)
Flo (3)
ELISA (23)
ICC (1)
IF (3)
IP (3)
Peptide-ELISA (2)
Host
rabbit (38)
mouse (23)
sheep (1)
Isotype
IgG (33)
IgG1 (15)
IgG2a (2)
IgG2b (4)
IgG2b,k (1)
Clonality
monoclonal mc (22)
polyclonal pc (40)
Clone
1A10 (1)
2G12 (2)
OTI2A4 (2)
OTI2D3 (2)
OTI2D9 (2)
OTI2G8 (2)
OTI3D8 (2)
OTI3E11 (2)
OTI3F6 (2)
OTI4A3 (2)
OTI5E5 (2)
Format
AP Conjugated (3)
APC Conjugated (3)
Azide-free (2)
Biotin Conjugated (5)
Carrier-free (9)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (3)
Unconjugated (38)
Epitope
aa473-729 (18)
aa608-636 (8)
C-Terminus (7)
aa575-624 (4)
1-80 aa, N-terminal (1)
Amino Acid: 435-658 (1)
N-Terminus (1)
Pro8 (1)
aa1-50 (1)
aa525-575 (1)
aa575-625 (1)
aa8-57 (1)
Publications
No (62)
Showing 1-25 of 62 results

IHC-plus™ C-TAK1 / MARK3 Antibody (aa1-50) LS-B7053
Rabbit Polyclonal (IgG) to Human C-TAK1 / MARK3
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $425.00
Anti-MARK3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot analysis of lysates from HeLa and 293 cells, using MARK3 Antibody. The lane on the right is blocked with the synthesized peptide.

C-TAK1 / MARK3 Antibody (clone 2G12) LS-C169131
Mouse Monoclonal [clone 2G12] (IgG1) to Human C-TAK1 / MARK3
Human
ELISA, Flo, IHC, IHC-P, WB
Unconjugated
100µg 1mg/ml  $365.00
IHC of paraffin-embedded bladder cancer tissues using MARK3 mouse monoclonal antibody with DAB staining.
Western blot using MARK3 monoclonal antibody against human MARK3 recombinant protein. (Expected MW is 40.8 kDa)

C-TAK1 / MARK3 Antibody (N-Terminus) LS-C356175
Rabbit Polyclonal to Human C-TAK1 / MARK3
Chicken, Zebrafish, Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of MARK3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of MARK3 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

C-TAK1 / MARK3 Antibody LS-C801895
Mouse Monoclonal (IgG1) to Human C-TAK1 / MARK3
Mouse, Human
ELISA, Flo, IHC, WB
Unconjugated
50µl 1mg/ml  $315.00; 100µl 1mg/ml  $375.00; 200µl 1mg/ml  $435.00

C-TAK1 / MARK3 Antibody (1-80 aa, N-terminal) LS-C382934
Rabbit Polyclonal (IgG) to Human C-TAK1 / MARK3
Mouse, Rat, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of C-TAK1 antibody
Western blot of C-TAK1 antibody

C-TAK1 / MARK3 Antibody LS-C678573
Rabbit Polyclonal (IgG) to Human C-TAK1 / MARK3
Human
ELISA, IF, IHC, IHC-P
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human small intestine tissue at dilution of 1:100
Immunofluorescent analysis of PC3 cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

C-TAK1 / MARK3 Antibody (Amino Acid: 435-658, clone 2G12) LS-C759661
Mouse Monoclonal [clone 2G12] (IgG1) to Human C-TAK1 / MARK3
Mouse, Human
Flo, IHC, WB
Unconjugated
100µg  $405.00
MARK3 Antibody in Immunohistochemistry (IHC)
MARK3 Antibody in Western Blot (WB)

C-TAK1 / MARK3 Antibody (aa473-729, clone OTI2G8) LS-C339713
Mouse Monoclonal [clone OTI2G8] (IgG1) to Human C-TAK1 / MARK3
Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Human pancreas tissue using anti-MARK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Western blot of extracts (10ug) from 2 different cell lines by using anti- MARK3monoclonal antibody at 1:200 dilution.

C-TAK1 / MARK3 Antibody (aa473-729, clone OTI2G8, Carrier-free) LS-C790702
Mouse Monoclonal [clone OTI2G8] (IgG1) to Human C-TAK1 / MARK3
Human
IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-MARK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY MARK3. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK3.

C-TAK1 / MARK3 Antibody (aa473-729, clone OTI2D9) LS-C339723
Mouse Monoclonal [clone OTI2D9] (IgG2a) to Human C-TAK1 / MARK3
Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Human liver tissue using anti-MARK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-MARK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

C-TAK1 / MARK3 Antibody (aa473-729, clone OTI2D9, Carrier-free) LS-C799557
Mouse Monoclonal [clone OTI2D9] (IgG2a) to Human C-TAK1 / MARK3
Human
IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-MARK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-MARK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,

C-TAK1 / MARK3 Antibody (aa473-729, clone OTI3F6) LS-C339747
Mouse Monoclonal [clone OTI3F6] (IgG1) to Human C-TAK1 / MARK3
Human
IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Human breast tissue using anti-MARK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY MARK3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK3.

C-TAK1 / MARK3 Antibody (aa473-729, clone OTI3F6, Carrier-free) LS-C790730
Mouse Monoclonal [clone OTI3F6] (IgG1) to Human C-TAK1 / MARK3
Human
IHC, WB
Unconjugated, Carrier-free
100µg  $395.00
Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-MARK3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
HEK293T cells were transfected with the pCMV6-ENTRY control. (Left lane) or pCMV6-ENTRY MARK3. (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates. (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-MARK3.

C-TAK1 / MARK3 Antibody (aa8-57) LS-C292167
Rabbit Polyclonal (IgG) to Human C-TAK1 / MARK3
Mouse, Rat, Human
IHC, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of the lysates from K562 cells using MARK3 antibody.

C-TAK1 / MARK3 Antibody (C-Terminus) LS-C319091
Rabbit Polyclonal (IgG) to Human C-TAK1 / MARK3
Human
ELISA, IHC
Unconjugated
200µl  $590.00

C-TAK1 / MARK3 Antibody (C-Terminus, Biotin) LS-C279259
Rabbit Polyclonal (IgG) to Human C-TAK1 / MARK3
Human
ELISA, IHC
Biotin Conjugated
200µl  $740.00

C-TAK1 / MARK3 Antibody (C-Terminus, FITC) LS-C279260
Rabbit Polyclonal (IgG) to Human C-TAK1 / MARK3
Human
ELISA, IHC
FITC Conjugated
200µl  $740.00

C-TAK1 / MARK3 Antibody (C-Terminus, AP) LS-C279257
Rabbit Polyclonal (IgG) to Human C-TAK1 / MARK3
Human
ELISA, IHC
AP Conjugated
200µl  $740.00

C-TAK1 / MARK3 Antibody (C-Terminus, APC) LS-C279258
Rabbit Polyclonal (IgG) to Human C-TAK1 / MARK3
Human
ELISA, IHC
APC Conjugated
200µl  $740.00

C-TAK1 / MARK3 Antibody (C-Terminus, Azide-free, HRP) LS-C279261
Rabbit Polyclonal (IgG) to Human C-TAK1 / MARK3
Human
ELISA, IHC
HRP Conjugated, Azide-free
200µl  $740.00

C-TAK1 / MARK3 Antibody (Pro8) LS-C191959
Rabbit Polyclonal to Human C-TAK1 / MARK3
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl  $345.00

C-TAK1 / MARK3 Antibody (aa608-636) LS-C322863
Rabbit Polyclonal (IgG) to Mouse C-TAK1 / MARK3
Mouse, Human
ELISA, WB
Unconjugated
200µl  $590.00

C-TAK1 / MARK3 Antibody (aa473-729, clone OTI3E11) LS-C339751
Mouse Monoclonal [clone OTI3E11] (IgG1) to Human C-TAK1 / MARK3
Human
IHC, IHC-P
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-MARK3 mouse monoclonal antibody. (heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).

C-TAK1 / MARK3 Antibody (clone 1A10) LS-C197651
Mouse Monoclonal [clone 1A10] (IgG2b,k) to Human C-TAK1 / MARK3
Human
ELISA, WB
Unconjugated
100µg  $365.00
Western Blot analysis of MARK3 expression in transfected 293T cell line by MARK3 monoclonal antibody (M01), clone 1A10.Lane 1: MARK3 transfected lysate(81.4 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged MARK3 is approximately 3 ng/ml as a capture antibody.

C-TAK1 / MARK3 Antibody LS-C666654
Rabbit Polyclonal (IgG) to Human C-TAK1 / MARK3
Human
ELISA, WB
Unconjugated
100µg  $335.00
Showing 1-25 of 62 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).