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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BRF1

BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit

General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.

Gene Name: BRF1, RNA polymerase III transcription initiation factor 90 kDa subunit
Synonyms: BRF1, B-related factor 1, BRF, B - related factor 1, HBRF, GTF3B, HTFIIIB90, TAF3C, TAFIII90, TFIIIB90, TAF3B2, BRF-1, TF3B90
Target Sequences: NM_001519 NP_001510.2 Q92994

Filtered by: None
Target
BRF1 (39)
Reactivity
Human (31)
Mouse (16)
Rat (6)
Bovine (4)
Application
IHC (9)
IHC-P (3)
WB (38)
DB (2)
ELISA (12)
ICC (3)
IF (7)
IP (2)
Peptide-ELISA (2)
Host
rabbit (34)
mouse (5)
Isotype
IgG (30)
IgG1 (3)
IgG1,k (1)
Clonality
monoclonal mc (4)
polyclonal pc (35)
Clone
2E9 (1)
BRF1G2A8 (2)
Format
AP Conjugated (2)
APC Conjugated (2)
Azide-free (1)
Biotin Conjugated (4)
FITC Conjugated (4)
HRP Conjugated (4)
PE Conjugated (2)
Unconjugated (21)
Epitope
aa314-342 (8)
C-Terminus (4)
aa551-600 (4)
N-Terminus (2)
aa627-677 (2)
200-280 aa, Internal (1)
Glu257 (1)
Internal (1)
aa208-257 (1)
aa231-280 (1)
aa550-600 (1)
Publications
No (39)
Showing 1-25 of 39 results

IHC-plus™ BRF1 Antibody (aa627-677) LS-B14055
Rabbit Polyclonal (IgG) to Human BRF1
Mouse, Human
IHC, IHC-P, IP, WB
Unconjugated
50µl 0.2mg/ml  $425.00
Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE)

BRF1 Antibody (Internal) LS-C352296
Rabbit Polyclonal to Human BRF1
Mouse, Rat, Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of BRF1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of BRF1 staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

BRF1 Antibody LS-C804579
Rabbit Polyclonal (IgG) to Human BRF1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunofluorescence Image

BRF1 Antibody (aa231-280) LS-C119195
Rabbit Polyclonal (IgG) to Human BRF1
Mouse, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TF3B Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of MCF7 cells, using TF3B Antibody. The picture on the right is blocked with the synthesized peptide.

BRF1 Antibody (200-280 aa, Internal) LS-C386387
Rabbit Polyclonal (IgG) to Human BRF1
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00
Western blot of TFIIIB90-1 antibody

BRF1 Antibody (clone BRF1G2A8) LS-C342330
Mouse Monoclonal [clone BRF1G2A8] (IgG1) to Human BRF1
Human
DB, ICC, IF, WB
Unconjugated
100µg 100µg/ml  $415.00
Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-BRF1 mAb.
Western blot analysis of immunized recombinant protein, using anti-BRF1 monoclonal antibody.

BRF1 Antibody (Glu257) LS-C177067
Rabbit Polyclonal to Human BRF1
Mouse, Human
IF, IHC, IHC-P, WB
Unconjugated
100µl  $345.00
IHC of TFIIIB90-1 (E257) pAb in paraffin-embedded human brain tissue.
Western blot of TFIIIB90-1 (E257) pAb in extracts from HeLa cells.

BRF1 Antibody (clone BRF1G2A8) LS-C184272
Mouse Monoclonal [clone BRF1G2A8] (IgG1) to Human BRF1
Human
DB, ICC, WB
Unconjugated
100µg  $560.00
Western blot of immunized recombinant protein using BRF1 antibody.

BRF1 Antibody (aa208-257) LS-C200186
Rabbit Polyclonal (IgG) to Human BRF1
Mouse, Human
IHC, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of the lysates from HepG2 cells using BRF1 antibody.

BRF1 Antibody (aa550-600) LS-C288039
Rabbit Polyclonal (IgG) to Human BRF1
Human
IHC, IP, WB
Unconjugated
100µl 0.2mg/ml  $395.00
Detection of Human BRF1 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRF1 used at a dilution of 1:200 (1 ug/ml).

BRF1 Antibody (clone 2E9) LS-C197381
Mouse Monoclonal [clone 2E9] (IgG1,k) to Human BRF1
Human
ELISA, WB
Unconjugated
50µg  $365.00
Detection limit for recombinant GST tagged BRF1 is 3 ng/ml as a capture antibody.

BRF1 Antibody (N-Terminus) LS-C46940
Rabbit Polyclonal to Human BRF1
Human
ELISA, WB
Unconjugated
100µg  $275.00

BRF1 Antibody (N-Terminus) LS-C46937
Rabbit Polyclonal to Human BRF1
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µg  $315.00

BRF1 Antibody (aa314-342) LS-C319876
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
Unconjugated
200µl  $590.00

BRF1 Antibody (aa314-342, Biotin) LS-C221561
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
Biotin Conjugated
200µl  $740.00

BRF1 Antibody (aa314-342, FITC) LS-C221562
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
FITC Conjugated
200µl  $740.00

BRF1 Antibody (aa314-342, AP) LS-C221559
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
AP Conjugated
200µl  $740.00

BRF1 Antibody (aa314-342, APC) LS-C221560
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
APC Conjugated
200µl  $740.00

BRF1 Antibody (aa314-342, PE) LS-C221565
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
PE Conjugated
200µl  $740.00

BRF1 Antibody (aa314-342, Azide-free, HRP) LS-C221563
Rabbit Polyclonal (IgG) to Human BRF1
Human
ELISA, WB
HRP Conjugated, Azide-free
200µl  $740.00

BRF1 Antibody LS-C306568
Mouse Polyclonal (IgG) to Human BRF1
Human
IF, WB
Unconjugated
50µg  $560.00

BRF1 Antibody LS-C77232
Mouse Monoclonal (IgG1) to Human BRF1
Human
WB
Unconjugated
100µg  $545.00

BRF1 Antibody (aa551-600) LS-C30226
Rabbit Polyclonal (IgG) to Mouse BRF1
Mouse
WB
Unconjugated
100µl 0.5mg/ml  $365.00
Thymus Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

BRF1 Antibody (C-Terminus) LS-C30602
Rabbit Polyclonal (IgG) to Mouse BRF1
Mouse, Bovine, Rat
WB
Unconjugated
100µl 0.5mg/ml  $335.00
NIH 3T3 Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

BRF1 Antibody (aa627-677) LS-C286515
Rabbit Polyclonal (IgG) to Human BRF1
Human
IHC
Unconjugated
100µl  $415.00
Detection of Human BRF1 by Immunohistochemistry. Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-BRF1 used at a dilution of 1:100.
Detection of Human BRF1 by Immunohistochemistry. Sample: FFPE section of human thyroid carcinoma. Antibody: Affinity purified rabbit anti-BRF1 used at a dilution of 1:500.
Showing 1-25 of 39 results

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).