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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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BMF

Bcl2 modifying factor

Apoptosis is related to many diseases and development. Members in the Bcl-2 family are critical regulators of apoptosis by either inhibiting or promoting cell death. Bcl-2 homology 3 (BH3) domain is a potent death domain. BH3-only proteins, including Bad, Bid, Bik, Hrk, Bim, Noxa, and PUMA, form a growing subclass of the Bcl-2 family. A novel BH3-only protein was recently identified in human and mouse and designated Bmf (for Bcl-2-modifying factor). The BH3 domain in Bmf is required both for binding to Bcl-2 proteins and for triggering apoptosis. In healthy cells, Bmf associates with the dynein light chain 2 (DLC2) component of the myosin V motors and is sequestered by the cell's actin cytoskeleton. Disruption of the actin cytoskeleton, either by depolymerization of actin filaments or by detachment of cells from the extracellular matrix, triggers release and activation of Bmf, initiating the downstream apoptotic program. Bmf is constitutively expressed in many tissues.

Gene Name: Bcl2 modifying factor
Family/Subfamily: Apoptosis
Synonyms: BMF, Bcl2 modifying factor, Bcl-2-modifying factor
Target Sequences: NM_033503 NP_277038.1 Q96LC9

Publications (2)

1
BH3 domains other than Bim and Bid can directly activate Bax/Bak. Du H, Wolf J, Schafer B, Moldoveanu T, Chipuk JE, Kuwana T. The Journal of biological chemistry. 2011 286:491-501. (WB; Mouse) [Full Text Article] [PubMed:21041309] [PMC:PMC3013008] Related Antibodies: LS-C4126.
2
2-deoxyglucose-induced toxicity is regulated by Bcl-2 family members and is enhanced by antagonizing Bcl-2 in lymphoma cell lines. Zagorodna O, Martin SM, Rutkowski DT, Kuwana T, Spitz DR, Knudson CM. Oncogene. 2012 May;31:2738-49. [Full Text Article] [PubMed:21986940] [PMC:PMC3257357] Related Antibodies: LS-C4126.

Viewing 1-25 of 56 items
BMF Antibody - Anti-BMF antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Select
Rabbit Polyclonal (IgG) to Human BMF
Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05mg   1mg/ml   $425.00
BMF Antibody - Anti-BMF antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Select
Rabbit Polyclonal (IgG) to Human BMF
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
50µg   0.5mg/ml   $555.00
Select
Rat Monoclonal [clone 12E10] (IgG1) to Mouse BMF
Mouse, Human
Flo, IP, WB
Unconjugated
100µg   1mg/ml   $710.00
Select
(2) Publication(s)
Rat Monoclonal [clone 9G10] (IgG2a) to Mouse BMF
Mouse, Human
Flo, IP, WB
Unconjugated
100µg   $680.00
BMF Antibody - Western blot using anti-Bmf (mouse/rat), mAb (17A9) detecting endogenous Bmf in mouse spleen, lymph node and thymus as two bands of ~25 and ~30 kDa, but not in tissues from two knock-out Bmf strains del 372 and del 376.
Select
Rat Monoclonal [clone 17A9] (IgG2a,k) to Mouse BMF
Mouse, Rat
ELISA, Flo, ICC, IHC, IP, WB
Unconjugated
100µg   $385.00
BMF Antibody - Immunohistochemistry of paraffin-embedded human liver cancer tissue.
Select
Rabbit Polyclonal (IgG) to Human BMF
Mouse, Rat, Human
IHC, WB
Unconjugated
50µl   $235.00; 100µl   $315.00; 200µl   $425.00
BMF Antibody - Western blot of BMF Antibody with human Ovary Tumor lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Select
Rabbit Polyclonal to Human BMF
Human
WB
Biotin Conjugated
100µl   1mg/ml   $385.00
Select
Rat Monoclonal [clone 17A9] (IgG2a) to Mouse BMF
Mouse
ELISA, Flo, IP, WB
Unconjugated
100µg   $680.00
BMF Antibody - IHC of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)(1:150).
Select
Mouse Monoclonal [clone OTI6F3] (IgG2b) to Human BMF
Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $345.00
BMF Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min. (1:150)
Select
Mouse Monoclonal [clone OTI6F3] (IgG2b) to Human BMF
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
BMF Antibody - IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)(1:150).
Select
Mouse Monoclonal [clone OTI9B7] (IgG1) to Human BMF
Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $345.00
BMF Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min. (1:150)
Select
Mouse Monoclonal [clone OTI9B7] (IgG1) to Human BMF
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
BMF Antibody - Immunocytochemistry of Bmf in HeLa cells with Bmf antibody at 10 µg/ml.
Select
Rabbit Polyclonal (IgG) to Human BMF
Mouse, Human
ELISA, ICC, WB
Unconjugated
0.1mg   1mg/ml   $365.00
BMF Antibody - IHC of paraffin-embedded Human Kidney tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min)(1:150).
Select
Mouse Monoclonal [clone OTI3H5] (IgG1) to Human BMF
Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $345.00
BMF Antibody - Immunohistochemical staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min. (1:150)
Select
Mouse Monoclonal [clone OTI3H5] (IgG1) to Human BMF
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
BMF Antibody - IHC of paraffin-embedded Human liver tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Select
Mouse Monoclonal [clone OTI3E5] (IgG1) to Human BMF
Human
IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $345.00
BMF Antibody - Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-BMF mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min,
Select
Mouse Monoclonal [clone OTI3E5] (IgG1) to Human BMF
Human
IHC, WB
Unconjugated, Carrier-free
100µg   $395.00
BMF Antibody - Immunofluorescence staining of BMF in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-human BMF polyclonal antibody (dilution ratio 1:5000) at 4°C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to cytoplasm and nucleus.
Select
Rabbit Polyclonal (IgG) to Human BMF
Human
ICC, IF, WB
Unconjugated, Preservative Free
50µl   $235.00; 100µl   $275.00; 200µl   $305.00
Select
Rat Monoclonal [clone 9G10] (IgG2a,k) to Mouse BMF
Mouse, Human
Flo, IP, WB
Unconjugated
100µg   $385.00
Select
Rabbit Polyclonal (IgG) to Human BMF
Human
ELISA, IHC, WB
Unconjugated
200µl   $590.00
Select
Rabbit Polyclonal (IgG) to Human BMF
Human
ELISA, IHC, WB
APC Conjugated
200µl   $740.00
BMF Antibody - Immunochemical staining of human BMF in human brain with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.
Select
Rabbit Polyclonal (IgG) to Human BMF
Human
ICC, IF, IHC-P
Unconjugated
50µl   $235.00; 100µl   $275.00; 200µl   $305.00
BMF Antibody - Immunohistochemistry of paraffin-embedded human colon cancer using antibody at 1:100 dilution.
Select
Rabbit Polyclonal (IgG) to Human BMF
Human
ELISA, IHC, IHC-P
Unconjugated
50µl   $285.00; 100µl   $385.00
BMF Antibody - Immunohistochemistry of paraffin-embedded human liver cancer using antibody at 1:100 dilution.
Select
Rabbit Polyclonal (IgG) to Human BMF
Human
ELISA, IHC, IHC-P
Unconjugated
50µl   $285.00; 100µl   $385.00
BMF Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Select
Rabbit Polyclonal to Human BMF
Human
IHC, IHC-P, WB
Unconjugated
400µl   $325.00

Viewing 1-25 of 56 items


If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).