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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
Infectious Disease
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BACH2

BTB and CNC homology 1, basic leucine zipper transcription factor 2

Transcriptional regulator that acts as repressor or activator. Binds to Maf recognition elements (MARE). Play important roles in coordinating transcription activation and repression by MAFK By similarity. Induces apoptosis in response to oxidative stress through repression of the antiapoptotic factor HMOX1.

Gene Name: BTB and CNC homology 1, basic leucine zipper transcription factor 2
Synonyms: BACH2, BTBD25, BTB and CNC homolog 2
Target Sequences: NM_021813 NP_068585.1 Q9BYV9

Publications (1)

1
Increase of T and B cells and altered BACH2 expression patterns in bone marrow trephines of imatinib-treated patients with chronic myelogenous leukaemia. Von Laffert M, Hänel M, Dietel M, Anagnostopoulos I, Jöhrens K. Oncology letters. 2016 October;12:2421-2428. (Human) [Full Text Article] [PubMed:27698808] [PMC:PMC5038374] Related Antibodies: LS-B3533.


BACH2 Antibody - Anti-BACH2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal (IgG) to Human BACH2
Rabbit, Human, Monkey
IHC, IHC-P, WB
Unconjugated
100µl   0.5mg/ml   $485.00
BACH2 Antibody - Anti-BACH2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal (IgG) to Human BACH2
Rabbit, Human, Monkey, Chimpanzee
IHC, IHC-P, WB
HRP Conjugated
100µl   0.65mg/ml   $405.00
BACH2 Antibody - Anti-BACH2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal (IgG) to Human BACH2
Rabbit, Human, Monkey, Chimpanzee
IHC, IHC-P, WB
Biotin Conjugated
100µl   1mg/ml   $405.00
BACH2 Antibody - Anti-BACH2 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal (IgG) to Human BACH2
Rabbit, Human, Monkey, Chimpanzee
IHC, IHC-P, WB
FITC Conjugated
100µl   1mg/ml   $405.00
BACH2 Antibody - Anti-BACH2 antibody IHC of human brain, cortex neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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Rabbit Polyclonal to Human BACH2
Mouse, Rat, Human
ELISA, IHC, IHC-P, WB
Unconjugated
100µl   1mg/ml   $425.00
BACH2 Antibody - Western Blot of Rabbit anti-Bach2 antibody. Lane 1: 293T cell lysates overexpressing Bach2-Flag. Lane 2: 293T cell lysates. Load: 20 ug per lane. Primary antibody: Bach-2 antibody at 1:1000 for overnight at 4 degrees C. Secondary antibody: rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4 degrees C. Predicted/Observed size: 91.7 kDa, ~130 kDa for Bach2. Other band(s): none.
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Rabbit Polyclonal (IgG) to Mouse BACH2
Mouse
ELISA, IP, WB
Unconjugated
100µg   0.97mg/ml   $425.00
BACH2 Antibody - Immunohistochemistry Dilution at 1:200 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal Goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated Secondary antibody and visualized using an HRP conjugated SP system.
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Rabbit Polyclonal (IgG) to Human BACH2
Human
ELISA, IF, IHC, IHC-P
Unconjugated
50µg   $285.00; 100µg   $385.00
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Rabbit Polyclonal (IgG) to Human BACH2
Human
ELISA
HRP Conjugated
50µg   $285.00; 100µg   $385.00
Select
Rabbit Polyclonal (IgG) to Human BACH2
Human
ELISA
Biotin Conjugated
50µg   0.25mg/ml   $285.00; 100µg   $385.00
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Rabbit Polyclonal (IgG) to Human BACH2
Human
ELISA
FITC Conjugated
50µg   $285.00; 100µg   $385.00
BACH2 Antibody - Western Blot of rabbit anti-Bach2 antibody. Lane 1: 293T cell lysates overexpressing Bach2-Flag. Lane 2: 293T cell lysates. Load: 20 µg per lane. Primary antibody: Bach-2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted (native)/Observed (over-expressed) size: 91.7 kDa, ~130 kDa for Bach2. Other band(s): none.
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Rabbit Polyclonal (IgG) to Mouse BACH2
Mouse
ELISA, IP, WB
Unconjugated
25µl   0.97mg/ml   $235.00
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Rabbit Polyclonal (IgG) to Human BACH2
Human
ELISA, WB
Unconjugated
200µl   $590.00
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Rabbit Polyclonal (IgG) to Human BACH2
Human
ELISA, WB
Biotin Conjugated
200µl   0.4mg/ml   $740.00
Select
Rabbit Polyclonal (IgG) to Human BACH2
Human
ELISA, WB
FITC Conjugated
200µl   $740.00
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Rabbit Polyclonal (IgG) to Human BACH2
Human
ELISA, WB
HRP Conjugated, Azide-free
200µl   0.4mg/ml   $740.00
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Rabbit Polyclonal (IgG) to Human BACH2
Human
ELISA, WB
AP Conjugated
200µl   $740.00
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Rabbit Polyclonal (IgG) to Human BACH2
Human
ELISA, WB
APC Conjugated
200µl   $740.00
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Rabbit Polyclonal (IgG) to Human BACH2
Human
ELISA, WB
PE Conjugated
200µl   $740.00
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Rabbit Polyclonal to Human BACH2
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50µg   $285.00; 100µg   1mg/ml   $355.00
BACH2 Antibody - 1:100 staining rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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Rabbit Polyclonal (IgG) to Human BACH2
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
100µl   1mg/ml   $325.00; 200µl   1mg/ml   $375.00
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Rabbit-New Zealand White Polyclonal to Human BACH2
Human
IHC, WB
Unconjugated
100µl   $325.00
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Rabbit Polyclonal to Human BACH2
Human
ELISA, WB
Unconjugated
100µg   100µl   1mg/ml   $305.00
BACH2 Antibody - Detection of human BACH2 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-BACH2 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
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Rabbit Polyclonal (IgG) to Human BACH2
Human
IP, WB
Unconjugated
100µl   1mg/ml   $405.00
BACH2 Antibody - BACH2 Antibody western blot of K562 cell line lysates (35 ug/lane). The BACH2 antibody detected the BACH2 protein (arrow).
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Rabbit Polyclonal to Human BACH2
Human
WB
Unconjugated
400µl   $325.00
BACH2 Antibody - BACH2 antibody Western blot of Fetal Thymus lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Rabbit Polyclonal to Human BACH2
Rabbit, Dog, Guinea pig, Horse, Human
WB
Unconjugated
100µl   0.5mg/ml   $385.00
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If you do not find the reagent or information you require, please contact Customer.Support@LSBio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).