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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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BACH1

BTB and CNC homology 1, basic leucine zipper transcription factor 1

BACH1 is a transcription factor that belongs to the cap'n'collar type of basic region leucine zipper factor family (CNC-bZip). The encoded protein contains broad complex, tramtrack, bric-a-brac/poxvirus and zinc finger (BTB/POZ) domains, which is atypical of CNC-bZip family members. These BTB/POZ domains facilitate protein-protein interactions and formation of homo- and/or hetero-oligomers. When this encoded protein forms a heterodimer with MafK, it functions as a repressor of Maf recognition element (MARE) and transcription is repressed. Multiple alternatively spliced transcript variants have been identified for this gene.

Gene Name: BTB and CNC homology 1, basic leucine zipper transcription factor 1
Synonyms: BACH1, BTB and CNC homolog 1, HA2303, BACH-1
Target Sequences: NM_001186 NP_001177.1 O14867

Filtered By: None
Target
BACH1 (34)
Reactivity
Human (34)
Mouse (2)
Chimpanzee (3)
Monkey (1)
Rabbit (4)
Application
IHC (10)
IHC-P (7)
WB (32)
Flo (1)
DB (2)
ELISA (13)
ICC (1)
IF (5)
IP (3)
Peptide-ELISA (3)
Host
rabbit (26)
mouse (5)
goat (3)
Isotype
IgG (22)
IgG1 (4)
IgG2a,k (1)
Clonality
monoclonal mc (5)
polyclonal pc (29)
Clone
1B8 (1)
4E11 (1)
BACAD04A (2)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
PE Conjugated (1)
Unconjugated (25)
Epitope
aa670-698 (8)
C-Terminus (4)
aa1-320 (2)
aa1233-1249 (2)
100-180 aa, Internal (1)
Internal (1)
Leu160 (1)
aa111-160 (1)
aa131-180 (1)
aa133-513 (1)
aa134-421 (1)
aa250-263 (1)
aa350-400 (1)
aa650-700 (1)
aa92-104 (1)
Publications
No (34)
Showing 1-25 of 34 results

BACH1 Antibody (aa92-104) IHC-plus™ LS-B332
Rabbit Polyclonal to Human BACH1
Human
ELISA IF IHC IHC-P WB
Unconjugated
50µg 1mg/ml  $425.00
Anti-BACH1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BACH1 Antibody - Western Blot. Western blot of Affinity Purified anti-BACH1 antibody shows detection of a band at ~105 kD (lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (arrowhead). Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction. Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
Anti-BACH1 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-BACH1 Antibody - Western Blot. Western blot of Affinity Purified anti-BACH1 antibody shows detection of a band at ~105 kD (lane 1) corresponding to human BACH1 present in a 293 whole cell lysate (arrowhead). Lane 2 shows that specific band staining is competed out when the antibody is pre-incubated with the peptide immunogen prior to reaction. Approximately 35 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1000. Reaction occurred 2 h at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX ( for 45 min at room temperature (800 nm channel, green). Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.

BACH1 Antibody (clone 4E11) LS-C173634
Mouse Monoclonal [clone 4E11] (IgG1) to Human BACH1
Human
Flo IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $345.00
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-BACH1 mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-BACH1 mouse monoclonal antibody.
IHC of paraffin-embedded Carcinoma of Human bladder tissue using anti-BACH1 mouse monoclonal antibody.
IHC of paraffin-embedded Human tonsil using anti-BACH1 mouse monoclonal antibody.

BACH1 Antibody (aa350-400) IHC-plus™ LS-B15079
Rabbit Polyclonal (IgG) to Human BACH1
Human
IHC IHC-P IP WB
Unconjugated
50µl 1mg/ml  $425.00
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)

BACH1 Antibody (Internal) LS-C351860
Rabbit Polyclonal to Human BACH1
Human, Monkey
ICC IF IHC IHC-P IP WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of BACH1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of BACH1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of BACH1 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of BACH1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

BACH1 Antibody (aa134-421) IHC-plus™ LS-B11616
Rabbit Polyclonal (IgG) to Human BACH1
Human
IHC IHC-P WB
Unconjugated
50µl 1mg/ml  $425.00
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)

BACH1 Antibody (C-Terminus) LS-C31733
Rabbit Polyclonal (IgG) to Human BACH1
Rabbit, Human
WB
Unconjugated
100µg  $325.00
A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
A: Marker, B: Jurkat Cell Lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

BACH1 Antibody (100-180 aa, Internal) LS-C382227
Rabbit Polyclonal (IgG) to Human BACH1
Human
ELISA IF IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Western blot of BACH1 antibody
Western blot of BACH1 antibody

BACH1 Antibody (aa1-320) LS-C375309
Rabbit Polyclonal (IgG) to Human BACH1
Human
ELISA IHC IHC-P WB
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human testis using antibody at 1:100 dilution.
Western blot. All lanes: BACH1 antibody at 2 ug/ml+mouse thymus tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 82 kDa. Observed band size: 82 kDa.
Immunohistochemistry of paraffin-embedded human testis using antibody at 1:100 dilution.
Western blot. All lanes: BACH1 antibody at 2 ug/ml+mouse thymus tissue. Secondary antibody: Goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 82 kDa. Observed band size: 82 kDa.

BACH1 Antibody (Leu160) LS-C176697
Rabbit Polyclonal to Human BACH1
Human
IF IHC WB
Unconjugated
100µl  $345.00
Western blot of BACH1 (L160) pAb in extracts from HeLa cells.
Western blot of BACH1 (L160) pAb in extracts from HeLa cells.

BACH1 Antibody LS-C659203
Rabbit Polyclonal (IgG) to Human BACH1
Human
ELISA IHC WB
Unconjugated
100µg  $405.00

BACH1 Antibody (aa1-320) LS-C375310
Rabbit Polyclonal (IgG) to Human BACH1
Human
ELISA IHC IHC-P
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human testis using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human testis using antibody at 1:100 dilution.

BACH1 Antibody (aa131-180) LS-C118663
Rabbit Polyclonal (IgG) to Human BACH1
Human
IF Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunofluorescence analysis of HeLa cells, using BACH1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HepG2 cells, using BACH1 Antibody. The lane on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HeLa cells, using BACH1 Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HepG2 cells, using BACH1 Antibody. The lane on the right is blocked with the synthesized peptide.

BACH1 Antibody (clone 1B8) LS-C196731
Mouse Monoclonal [clone 1B8] (IgG2a,k) to Human BACH1
Mouse, Human
ELISA WB
Unconjugated
100µg 1mg/ml  $355.00
BACH1 monoclonal antibody (M02), clone 1B8 Western Blot analysis of BACH1 expression in NIH/3T3.
Detection limit for recombinant GST tagged BACH1 is approximately 0.03 ng/ml as a capture antibody.
BACH1 monoclonal antibody (M02), clone 1B8 Western Blot analysis of BACH1 expression in NIH/3T3.
Detection limit for recombinant GST tagged BACH1 is approximately 0.03 ng/ml as a capture antibody.

BACH1 Antibody (aa670-698) LS-C319748
Rabbit Polyclonal (IgG) to Human BACH1
Human
ELISA WB
Unconjugated
200µl  $530.00

BACH1 Antibody (aa670-698, Biotin) LS-C252233
Rabbit Polyclonal (IgG) to Human BACH1
Human
ELISA WB
Biotin Conjugated
200µl  $680.00

BACH1 Antibody (aa670-698, FITC) LS-C252232
Rabbit Polyclonal (IgG) to Human BACH1
Human
ELISA WB
FITC Conjugated
200µl  $680.00

BACH1 Antibody (aa670-698, APC) LS-C252231
Rabbit Polyclonal (IgG) to Human BACH1
Human
ELISA WB
APC Conjugated
200µl  $680.00

BACH1 Antibody (aa670-698, Azide-free, HRP) LS-C252234
Rabbit Polyclonal (IgG) to Human BACH1
Human
ELISA WB
HRP Conjugated, Azide-free
200µl  $680.00

BACH1 Antibody (aa670-698, PE) LS-C252235
Rabbit Polyclonal (IgG) to Human BACH1
Human
ELISA WB
PE Conjugated
200µl  $680.00

BACH1 Antibody (aa670-698, AP) LS-C252230
Rabbit Polyclonal (IgG) to Human BACH1
Human
ELISA WB
AP Conjugated
200µl  $680.00

BACH1 Antibody (clone BACAD04A) LS-C342328
Mouse Monoclonal [clone BACAD04A] (IgG1) to Human BACH1
Human
DB WB
Unconjugated
100µg 0.1mg/ml  $375.00
Western blot analysis of immunized recombinant protein, using anti-BACH1 monoclonal antibody.
Western blot analysis of immunized recombinant protein, using anti-BACH1 monoclonal antibody.

BACH1 Antibody (aa650-700) LS-C289759
Rabbit Polyclonal (IgG) to Human BACH1
Human
IP WB
Unconjugated
100µl 1mg/ml  $395.00

BACH1 Antibody (aa111-160) LS-C200172
Rabbit Polyclonal (IgG) to Human BACH1
Human
Peptide-ELISA WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Western blot analysis of the lysates from HeLa cells using BACH1 antibody.
Western blot analysis of the lysates from HeLa cells using BACH1 antibody.

BACH1 Antibody (aa250-263) LS-C186445
Goat Polyclonal to Human BACH1
Human
Peptide-ELISA WB
Unconjugated
100µg 0.5mg/ml  $405.00
BACH1 antibody (1 ug/ml) staining of Human Peripheral Blood Lymphocytes (A) and Human Spleen (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
BACH1 antibody (1 ug/ml) staining of Human Peripheral Blood Lymphocytes (A) and Human Spleen (B) lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

BACH1 Antibody LS-C77319
Mouse Monoclonal (IgG1) to Human BACH1
Human
WB
Unconjugated
100µg  $545.00
Showing 1-25 of 34 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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