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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AUTL1 / ATG4C

autophagy related 4C, cysteine peptidase

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation (By similarity). Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

Gene Name: autophagy related 4C, cysteine peptidase
Synonyms: ATG4C, AUTL3, APG4-C, APG4C, AUTL1, Autophagin-3, APG4 autophagy 4 homolog C, Cysteine protease ATG4C
Target Sequences: NM_032852 NP_116241.2 Q96DT6

Publications (1)

1
Temporal orchestration of circadian autophagy rhythm by C/EBP. Ma D, Panda S, Lin JD. The EMBO journal. 2011 30:4642-51. [PubMed:21897364] [PMC:PMC3243590] Related Antibodies: LS-C156598.

Filtered by: None
Target
AUTL1 / ATG4C (75)
Reactivity
Human (67)
Mouse (34)
Rat (22)
Bovine (4)
Chimpanzee (3)
Gibbon (3)
Horse (4)
Monkey (2)
Application
IHC (16)
IHC-P (8)
WB (69)
Flo (1)
ELISA (21)
ICC (1)
IF (44)
Peptide-ELISA (1)
Host
rabbit (64)
mouse (10)
sheep (1)
Isotype
IgG (19)
IgG1 (1)
IgG1,k (8)
Clonality
monoclonal mc (9)
polyclonal pc (66)
Clone
287CT12.2.2 (7)
2E10H7 (1)
Format
AP Conjugated (3)
APC Conjugated (3)
Atto 390 Conjugated (2)
Atto 488 Conjugated (2)
Atto 565 Conjugated (2)
Atto 594 Conjugated (4)
Atto 633 Conjugated (2)
Atto 655 Conjugated (2)
Atto 680 Conjugated (1)
Atto 700 Conjugated (2)
Azide-free (1)
Biotin Conjugated (5)
FITC Conjugated (5)
HRP Conjugated (5)
PE (2)
PE Conjugated (1)
PerCP Conjugated (2)
RPE Conjugated (2)
SA Conjugated (2)
Unconjugated (30)
Epitope
aa175-224 (4)
40-120 aa, N-terminal (1)
C-Terminus (1)
N-Terminus (1)
aa167-196 (1)
aa21-70 (1)
aa259-458 (1)
aa33-62 (1)
aa419-448 (1)
aa51-65 (1)
Publications
No (74)
Yes (1)
Showing 1-25 of 75 results

IHC-plus™ AUTL1 / ATG4C Antibody LS-B12153
Rabbit Polyclonal (IgG) to Human AUTL1 / ATG4C
Mouse, Rat, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05mg 1mg/ml  $425.00
Human Brain, Cortex: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

AUTL1 / ATG4C Antibody (aa419-448) LS-C156598
Publications (1)
Publications (1)
Rabbit Polyclonal to Human AUTL1 / ATG4C
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of lysates from HeLa cell line and mouse liver tissue lysate(from left to right), using APG4C Antibody. Antibody was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.

AUTL1 / ATG4C Antibody (aa33-62) LS-C156600
Rabbit Polyclonal to Human AUTL1 / ATG4C
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-hAPG4C-Y48 antibody in 293 cell line lysates transiently transfected with the ATG4C gene (2 ug/lane). hAPG4C-Y48(arrow) was detected using the purified antibody.

AUTL1 / ATG4C Antibody LS-C93849
Sheep Polyclonal (IgG) to Human AUTL1 / ATG4C
Mouse, Rat, Human
IHC, WB
Unconjugated
500µg  $325.00

AUTL1 / ATG4C Antibody (N-Terminus) LS-C354550
Rabbit Polyclonal to Human AUTL1 / ATG4C
Human
ICC, IF, IHC, IHC-P, WB
Unconjugated
100µl 1mg/ml  $245.00
Immunohistochemical analysis of ATG4C staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX. w
Immunofluorescent analysis of ATG4C staining in K562 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

AUTL1 / ATG4C Antibody LS-C804986
Rabbit Polyclonal (IgG) to Human AUTL1 / ATG4C
Mouse, Human
ELISA, IF, IHC, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Immunohistochemistry Image
Immunofluorescence Image

AUTL1 / ATG4C Antibody (aa21-70) LS-C119887
Rabbit Polyclonal (IgG) to Human AUTL1 / ATG4C
Human
IF, IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50µl 1mg/ml  $265.00; 100µl 1mg/ml  $325.00
Immunohistochemistry analysis of paraffin-embedded human liver carcinoma tissue, using ATG4C Antibody. The picture on the right is blocked with the synthesized peptide.
Immunofluorescence analysis of HUVEC cells, using ATG4C Antibody. The picture on the right is blocked with the synthesized peptide.

AUTL1 / ATG4C Antibody (40-120 aa, N-terminal) LS-C382183
Rabbit Polyclonal (IgG) to Human AUTL1 / ATG4C
Human, Monkey
ELISA, IF, IHC, WB
Unconjugated
50µl 1mg/ml  $235.00; 100µl 1mg/ml  $265.00; 200µl 1mg/ml  $335.00

AUTL1 / ATG4C Antibody LS-C659918
Rabbit Polyclonal (IgG) to Human AUTL1 / ATG4C
Human
ELISA, IF, IHC, WB
Unconjugated
100µg  $405.00

AUTL1 / ATG4C Antibody (aa259-458) LS-C378345
Rabbit Polyclonal (IgG) to Human AUTL1 / ATG4C
Mouse, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human breast cancer using ATG4C Antibody at dilution of 1:100

AUTL1 / ATG4C Antibody LS-C501376
Rabbit Polyclonal (IgG) to Human AUTL1 / ATG4C
Human
ELISA, IF, IHC
Unconjugated
50µg  $285.00; 100µg  $385.00
Immunohistochemistry of paraffin-embedded human testis tissue using ATG4C Antibody at dilution of 1:100
Immunohistochemistry of paraffin-embedded human heart tissue using ATG4C Antibody at dilution of 1:100

AUTL1 / ATG4C Antibody LS-C772271
Rabbit Polyclonal (IgG) to Human AUTL1 / ATG4C
Human, Monkey
ELISA, IF, IHC-P, WB
Unconjugated
20µl 1mg/ml  $205.00; 60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

AUTL1 / ATG4C Antibody LS-C401307
Rabbit Polyclonal (IgG) to Human AUTL1 / ATG4C
Mouse, Human
ELISA, IHC, WB
Unconjugated
20µl 0.5mg/ml  $205.00; 60µl 0.5mg/ml  $235.00; 120µl 0.5mg/ml  $295.00; 200µl 0.5mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human brain using ATG4C Polyclonal Antibody at dilution of 1:20.
Immunohistochemistry of paraffin-embedded Human thyroid cancer using ATG4C Polyclonal Antibody at dilution of 1:20.

AUTL1 / ATG4C Antibody LS-C404582
Rabbit Polyclonal (IgG) to Human AUTL1 / ATG4C
Mouse, Human
ELISA, IHC, WB
Unconjugated
20µl 0.3mg/ml  $205.00; 60µl 0.3mg/ml  $235.00; 120µl 0.3mg/ml  $295.00; 200µl 0.3mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human prostate cancer using ATG4C Polyclonal Antibody at dilution of 1:25.
Immunohistochemistry of paraffin-embedded Human thyroid cancer using ATG4C Polyclonal Antibody at dilution of 1:25.

AUTL1 / ATG4C Antibody (aa167-196) LS-C156599
Rabbit Polyclonal to Human AUTL1 / ATG4C
Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
The anti-APG4C antibody is used in Western blot to detect APG4C in HeLa tissue lysate

AUTL1 / ATG4C Antibody (clone 2E10H7) LS-C682508
Mouse Monoclonal [clone 2E10H7] (IgG1) to Human AUTL1 / ATG4C
Human
ELISA, WB
Unconjugated
100µl  $365.00
Western blot analysis using ATG4C mAb against HEK293 (1) and ATG4C (AA: 321-458)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot analysis using ATG4C mAb against human ATG4C (AA: 321-458) recombinant protein. (Expected MW is 42.2 kDa)

AUTL1 / ATG4C Antibody LS-C775399
Rabbit Polyclonal to Human AUTL1 / ATG4C
Mouse, Rat, Human
IF, WB
Unconjugated
100µg 1mg/ml  $365.00
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.

AUTL1 / ATG4C Antibody (clone 287CT12.2.2) LS-C319116
Mouse Monoclonal [clone 287CT12.2.2] (IgG1,k) to Mouse AUTL1 / ATG4C
Mouse
ELISA, WB
Unconjugated
200µl  $590.00

AUTL1 / ATG4C Antibody LS-C192357
Rabbit Polyclonal to Human AUTL1 / ATG4C
Mouse, Rat, Human
IHC, WB
Unconjugated
100µl  $385.00
Western blot of testis cell lysate, using ATG4C antibody.

AUTL1 / ATG4C Antibody LS-C349116
Rabbit Polyclonal (IgG) to Human AUTL1 / ATG4C
Human
IHC, WB
Unconjugated
50µl  $235.00; 100µl  $315.00; 200µl  $425.00
Western blot analysis of extracts of various cell lines.

AUTL1 / ATG4C Antibody LS-C773882
Rabbit Polyclonal to Human AUTL1 / ATG4C
Human
IF, WB
Unconjugated
100µg 1mg/ml  $365.00
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Colon carcinoma cell line (RKO). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) ATG4C Antibody. (D) Composite.
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of 53 kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HEK293T cell lysates. Load: 15 µg. Block: 2% GE Ashermsham in 1X TBST. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 16 hours at 4°C. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 minutes in RT. Predicted/Observed Size: 53 kDa.

AUTL1 / ATG4C Antibody (HRP) LS-C775398
Rabbit Polyclonal to Human AUTL1 / ATG4C
Mouse, Rat, Human
IF, WB
HRP Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.

AUTL1 / ATG4C Antibody (AP) LS-C775396
Rabbit Polyclonal to Human AUTL1 / ATG4C
Mouse, Rat, Human
IF, WB
AP Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.

AUTL1 / ATG4C Antibody (PerCP) LS-C775400
Rabbit Polyclonal to Human AUTL1 / ATG4C
Mouse, Rat, Human
IF, WB
PerCP Conjugated
100µg 1mg/ml  $395.00
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.

AUTL1 / ATG4C Antibody (Atto 390) LS-C775402
Rabbit Polyclonal to Human AUTL1 / ATG4C
Mouse, Rat, Human
IF, WB
Atto 390 Conjugated
100µg 1mg/ml  $405.00
Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-ATG4C Polyclonal Antibody. Tissue: Myoblast cell line (C2C12). Species: Mouse. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cytoplasm. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ATG4C Antibody (D) Composite.
Western blot analysis of Human Embryonic kidney epithelial cell line (HEK293T) lysate showing detection of ~52.5kDa ATG4C protein using Rabbit Anti-ATG4C Polyclonal Antibody. Lane 1: MW Ladder. Lane 2: Human 293T (20 µg). Load: 20 µg. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-ATG4C Polyclonal Antibody  at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~52.5kDa.
Showing 1-25 of 75 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).