LSBio
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA and Assay Kits
  • All Kits
  • Assay Kits
  • Traditional ELISA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Phospho-Specific ELISA Kits
  • ELISA Development Kits
  • Chemiluminescent CLIA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Synthetic Peptides
  • Biochemicals
  • All Biochemicals

  • Immunohistochemistry
  • Immunohistochemistry Reagents
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Research Areas
  • Cancer Research:
  • Breast Cancer IHC
  • Colorectal Cancer IHC
  • Lung Cancer IHC
  • Prostate Cancer IHC
  • Cell Cycle:
  • Apoptosis
  • Protein Family and Group:
  • Caspases
  • Chemokines
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Reviews
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Immunohistochemistry Protocol
  • Protocols
  • Secure Logins
  • Login to Our Secure ShareFile Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Login ▾
Wish List
View Cart

Unfortunately, the antibody (ID:40914) is no longer available.

Below is a list of antibodies to the same protein target. You may also try your search again using the search box at the top of the page. Please contact us if you have any questions.



AURKA / Aurora-A

aurora kinase A

Mitotic serine/threonine kinases that contributes to the regulation of cell cycle progression. Associates with the centrosome and the spindle microtubules during mitosis and plays a critical role in various mitotic events including the establishment of mitotic spindle, centrosome duplication, centrosome separation as well as maturation, chromosomal alignment, spindle assembly checkpoint, and cytokinesis. Required for initial activation of CDK1 at centrosomes. Phosphorylates numerous target proteins, including ARHGEF2, BORA, BRCA1, CDC25B, DLGP5, HDAC6, KIF2A, LATS2, NDEL1, PARD3, PPP1R2, PLK1, RASSF1, TACC3, p53/TP53 and TPX2. Regulates KIF2A tubulin depolymerase activity. Required for normal axon formation. Plays a role in microtubule remodeling during neurite extension. Important for microtubule formation and/or stabilization. Also acts as a key regulatory component of the p53/TP53 pathway, and particularly the checkpoint-response pathways critical for oncogenic transformation of cells, by phosphorylating and stabilizing p53/TP53. Phosphorylates its own inhibitors, the protein phosphatase type 1 (PP1) isoforms, to inhibit their activity. Necessary for proper cilia disassembly prior to mitosis.

Gene Name: aurora kinase A
Family/Subfamily: Protein Kinase , Aurora/IPL1-like
Synonyms: AURKA, AIK, AIRK1, ARK1, Aurora-A kinase, Aurora kinase A, Aurora/IPL1-like kinase, AYK1, AURA, AURORA2, Breast tumor-amplified kinase, Breast-tumor-amplified kinase, BTAK, HARK1, IPL1-related kinase, Serine/threonine kinase 6, STK7, STK6, PPP1R47, ARK-1, Aurora 2, Aurora-A, Aurora-related kinase 1, Aurora/IPL1-related kinase 1, IAK1, Serine/threonine kinase 15, STK15
Target Sequences: NM_003600 NP_003591.2 O14965

Publications (4)

1
Specialized roles of the two mitotic cyclins in somatic cells: cyclin A as an activator of M phase-promoting factor. Fung TK, Ma HT, Poon RY. Molecular biology of the cell. 2007 18:1861-73. [PubMed:17344473] [PMC:PMC1855023]
2
Cotreatment with vorinostat enhances activity of MK-0457 (VX-680) against acute and chronic myelogenous leukemia cells. Fiskus W, Wang Y, Joshi R, Rao R, Yang Y, Chen J, Kolhe R, Balusu R, Eaton K, Lee P, Ustun C, Jillella A, Buser CA, Peiper S, Bhalla K. Clinical cancer research : an official journal of the American Association for Cancer Research. 2008 14:6106-15. (WB) [PubMed:18829489] [PMC:PMC2665710]
3
Curcumin-induced mitotic spindle defect and cell cycle arrest in human bladder cancer cells occurs partly through inhibition of aurora A. Liu HS, Ke CS, Cheng HC, Huang CY, Su CL. Molecular pharmacology. 2011 80:638-46. [PubMed:21757545]
4
Expression of aurora kinase A and B in chondrosarcoma and its relationship with the prognosis. Liang X, Wang D, Wang Y, Zhou Z, Zhang J, Li J. Diagnostic pathology. 2012 7:84. [PubMed:22809428] [PMC:PMC3487823]
more

Filtered By: None
Target
aurka-aurora-a (84)
Reactivity
Human (73)
Mouse (35)
Rat (21)
Bovine (9)
Chimpanzee (6)
Dog (4)
Gibbon (3)
Guinea pig (4)
Horse (4)
Monkey (7)
Orangutan (3)
Pig (2)
Primate (1)
Rabbit (4)
Sheep (9)
Zebrafish (4)
Application
IHC (40)
IHC-Fr (1)
IHC-P (17)
WB (66)
Flo (4)
ELISA (32)
ICC (10)
IF (16)
IP (11)
Peptide-ELISA (5)
Host
rabbit (70)
mouse (13)
goat (1)
Isotype
IgG (43)
IgG1 (4)
IgG1,k (2)
IgG2a,k (1)
IgG2b (3)
IgM (1)
Clonality
monoclonal mc (13)
polyclonal pc (71)
Clone
1364CT291.108.155 (1)
1F8 (2)
2D8A4 (1)
3H1 (1)
4G10 (1)
4H5A8 (1)
5F8 (1)
7A3 (1)
Format
Agarose Conjugated (3)
AP Conjugated (2)
APC Conjugated (2)
Azide-free (2)
Biotin Conjugated (6)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (2)
Unconjugated (59)
Epitope
pThr288 (10)
aa90-120 (8)
aa93-120 (8)
C-Terminus (7)
N-Terminus (4)
aa151-200 (4)
aa75-125 (3)
aa50-100 (2)
pSer342 (2)
Pro282 (1)
Tyr338 (1)
aa100-150 (1)
aa109-125 (1)
aa230-310 (1)
aa256-305 (1)
aa280-360 (1)
aa311-360 (1)
aa394-403 (1)
pSer241 (1)
Publications
No (84)
Showing 1-25 out of 84 results
AURKA / Aurora-A Antibody (aa394-403) IHC-plus™ LS-B191
Anti-AURKA / Aurora-A antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B191 concentration 10 ug/ml.

Goat Polyclonal to Human AURKA / Aurora-A
Human
IHC IHC-P IP Peptide-ELISA WB
Unconjugated
AURKA / Aurora-A Antibody (aa100-150) IHC-plus™ LS-B6517
Anti-AURKA / Aurora-A antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6517 concentration 10 ug/ml.
Western blot of AIK1 in A) HCT-116, B) human testis, C) mouse testis, and D) rat testis lysate using antibody at 2 ug/ml.

Rabbit Polyclonal to Human AURKA / Aurora-A
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
AURKA / Aurora-A Antibody (phospho-Thr288) LS-C205304
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using Aurora A (Phospho-Thr288) antibody (left) or the same antibody preincubated with blocking peptide (right).

Rabbit Polyclonal to Human AURKA / Aurora-A
Mouse, Human
IHC IHC-P
Unconjugated
AURKA / Aurora-A Antibody (clone 7A3) IHC-plus™ LS-B3912
Anti-AURKA / Aurora-A antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3912 concentration 10 ug/ml.
Panel A Hela cells stained with anti-Aurora A (clone 7A3) and FITC-conjugate goat anti-mouse antibody. Panel B: Hela cells stained with DAPI to indicate nuclei.

Mouse Monoclonal [clone 7A3] (IgM) to Human AURKA / Aurora-A
Human
IF IHC IHC-P WB
Unconjugated
AURKA / Aurora-A Antibody (clone 2D8A4) LS-C682496
Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using AURKA mouse mAb with DAB staining.
Immunofluorescence analysis of SMMC-7721 cells using AURKA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin. Secondary antibody from Fisher

Mouse Monoclonal [clone 2D8A4] (IgG2b) to Human AURKA / Aurora-A
Human
ELISA Flo ICC IHC WB
Unconjugated
AURKA / Aurora-A Antibody LS-C137035

Rabbit Polyclonal (IgG) to Human AURKA / Aurora-A
Human
ELISA WB
Unconjugated
AURKA / Aurora-A Antibody (clone 1F8) LS-C359318
Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) - Immunofluorescence analysis of HeLa cells using Aurora A mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Western Blot: Aurora A Antibody (1F8) - Western blot analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), HeLa (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysate.

Mouse Monoclonal [clone 1F8] (IgG1) to Human AURKA / Aurora-A
Rat, Primate, Human
ELISA Flo ICC IF WB
Unconjugated
AURKA / Aurora-A Antibody (phospho-Thr288) LS-C358422
Immunohistochemical analysis of Aurora A (pT288) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP-conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Aurora A (pT288) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Rabbit Polyclonal to Human AURKA / Aurora-A
Mouse, Sheep, Bovine, Rat, Pig, Human, Monkey
ICC IF IHC IHC-P WB
Unconjugated
AURKA / Aurora-A Antibody (C-Terminus) LS-C354094
Immunohistochemical analysis of Aurora A staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Immunofluorescent analysis of Aurora A staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Rabbit Polyclonal to Human AURKA / Aurora-A
Mouse, Rat, Pig, Human, Monkey
ICC IF IHC IHC-P WB
Unconjugated
AURKA / Aurora-A Antibody LS-C677261
Immunohistochemistry of paraffin-embedded human ovarian cancer at dilution of 1:100
Immunofluorescent analysis of HeLa cells diluted at 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Rabbit Polyclonal (IgG) to Human AURKA / Aurora-A
Human
ELISA IF IHC WB
Unconjugated
AURKA / Aurora-A Antibody (aa280-360) LS-C382140
Western blot of ARK-1 antibody

Rabbit Polyclonal (IgG) to Human AURKA / Aurora-A
Mouse, Rat, Human, Monkey
ELISA IF IHC WB
Unconjugated
AURKA / Aurora-A Antibody (C-Terminus) IHC-plus™ LS-B6732
Anti-AURKA / Aurora-A antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6732 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
293T cell lysate. Antibody concentration: 1.0 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

Rabbit Polyclonal (IgG) to Human AURKA / Aurora-A
Sheep, Bovine, Human
IHC IHC-P WB
Unconjugated
AURKA / Aurora-A Antibody (Tyr338) LS-C176675
IHC of AurA (Y338) pAb in paraffin-embedded human breast carcinoma.
Western blot of AurA (Y338) pAb in extracts from COS7 cells.

Rabbit Polyclonal to Human AURKA / Aurora-A
Mouse, Rat, Human
IF IHC IHC-P WB
Unconjugated
AURKA / Aurora-A Antibody (clone 4H5A8) LS-C682498
Western blot analysis using AURKA mouse mAb against HEK293 (1), PC-12 (2), and HT-29 (3) cell lysate.
Western blot analysis using AURKA mAb against human AURKA (AA: 268-404) recombinant protein. (Expected MW is 41.5 kDa)

Mouse Monoclonal [clone 4H5A8] (IgG1) to Human AURKA / Aurora-A
Human
ELISA IHC WB
Unconjugated
AURKA / Aurora-A Antibody LS-C180105
Immunohistochemistry: Aurora A Antibody - IHC of Aurora A in mouse brain.
Western Blot: Aurora A Antibody - WB detection of Aurora A in HeLa whole cell lysate.

Rabbit Polyclonal to Human AURKA / Aurora-A
Mouse, Human
ICC IHC IHC-P WB
Unconjugated
AURKA / Aurora-A Antibody (clone 5F8) LS-C133953
Immunofluorescence of monoclonal antibody to STK6 on HeLa cell. [antibody concentration 30 ug/ml]
STK6 monoclonal antibody (M01), clone 5F8 Western blot of STK6 expression in HeLa NE.

Mouse Monoclonal [clone 5F8] (IgG2a,k) to Human AURKA / Aurora-A
Human
ELISA IF WB
Unconjugated
AURKA / Aurora-A Antibody (C-Terminus) LS-C287011
Detection of Rat Aurora A by Western Blot. Samples: Whole tissue lysate (60 ug/lane) from rat mammary glands. Antibody: Affinity purified rabbit anti-mouse Aurora A antibody used at 0.2 ug/ml. Detection: Chemiluminescence with an exposure time of 6 minutes. *Note: The upper band is believed to be phosphorylated Aurora A.

Rabbit Polyclonal (IgG) to Mouse AURKA / Aurora-A
Mouse, Rat
ICC IF WB
Unconjugated
AURKA / Aurora-A Antibody (clone 4G10) LS-C169311
IHC of paraffin-embedded ovarian cancer (left) and lung cancer (right) using AURKA mouse monoclonal antibody with DAB staining.
Western blot using AURKA mouse monoclonal antibody against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), HeLa (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysate.

Mouse Monoclonal [clone 4G10] (IgG1) to Human AURKA / Aurora-A
Human
ELISA IHC WB
Unconjugated
AURKA / Aurora-A Antibody LS-C12051

Rabbit Polyclonal to Human AURKA / Aurora-A
Human
IHC IHC-Fr IP WB
Unconjugated
AURKA / Aurora-A Antibody (clone 3H1) LS-C204509
Shows HeLa cell cultures stained with AURKA / Aurora-A antibody antibody (green). Aurora A localizes in spindle poles and mitotic spindles at anaphase and concentrates on the midbody between the two daughter cells during telophase. Counterstained is a chicken polyclonal antibody against Vimentin (red). Blue is a DNA stain.
Western analysis of AURKA / Aurora-A antibody. Blot of HeLa cells treated with 100 ng/mL of nocodazole for 18 hours was probed with AURKA / Aurora-A antibody. Nocodazole is a microtubule inhibitor which induces cells G2/M phase and induces Aurora A expression. The AURKA / Aurora-A antibody monoclonal binds strongly and cleanly to a band at about 46 kDa.

Mouse Monoclonal [clone 3H1] (IgG1,k) to Human AURKA / Aurora-A
Human
ICC IF WB
Unconjugated
AURKA / Aurora-A Antibody LS-C497939
Western blot analysis of extracts of A2058 cells.

Rabbit Polyclonal (IgG) to Human AURKA / Aurora-A
Human
Flo IF WB
Unconjugated
AURKA / Aurora-A Antibody (phospho-Thr288) LS-C198663
IHC of paraffin-embedded human breast carcinoma, using Aurora Kinase (Phospho-Thr288) Antibody. The sample on the right was incubated with synthetic peptide.
Western blot of extracts from 293 cells treated with serum 20% 15', using Aurora Kinase (Phospho-Thr288) Antibody. The lane on the right was incubated with synthetic peptide.

Rabbit Polyclonal (IgG) to Human AURKA / Aurora-A
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
AURKA / Aurora-A Antibody (aa90-120) LS-C99458
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Formalin-fixed and paraffin-embedded human colon carcinoma tissue reacted with AIK antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Rabbit Polyclonal to Human AURKA / Aurora-A
Human
IHC IHC-P WB
Unconjugated
AURKA / Aurora-A Antibody (aa90-120) LS-C326899

Rabbit Polyclonal (IgG) to Human AURKA / Aurora-A
Human
ELISA IHC WB
Unconjugated
AURKA / Aurora-A Antibody LS-C664380

Rabbit Polyclonal (IgG) to Human AURKA / Aurora-A
Human
ELISA IHC WB
Unconjugated

Selected:

If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).


Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn PSL Alliance Member
Copyright © 2018 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy



Catalog Number