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ATP5A1 / ATP Synthase Alpha

ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle

Mitochondrial membrane ATP synthase (F1F0 ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F1 - containing the extramembraneous catalytic core, and F0 - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F1 is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F1. Rotation of the central stalk against the surrounding alpha3beta3 subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites.

Gene Name: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
Family/Subfamily: Transporter , ATPase - F type
Synonyms: ATP5A1, ATP5AL2, ATP5A, HATP1, Mitochondrial ATP synthase, MOM2, ORM, OMR, ATPM
Target Sequences: NM_004046 NP_004037.1 P25705

Publications (1)

1
Taurine Inhibits Glucocorticoid-Induced Bone Mitochondrial Injury, Preventing Osteonecrosis in Rabbits and Cultured Osteocytes. Hiroaki Hirata, Shusuke Ueda, Toru Ichiseki, Miyako Shimasaki, Yoshimichi Ueda, Ayumi Kaneuji, Norio Kawahara. International journal of molecular sciences. 2020 September;21: [Full Text Article] [PubMed:32962196] [PMC:PMC7555938] Related Antibodies: LS-B11269.

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Antibodies (28)
Primary (28)
ATP5A1 / ATP Synthase Alpha (28)
Human (28)
Mouse (13)
Rat (10)
Bat (1)
Bovine (1)
Hamster (1)
Horse (1)
Monkey (1)
Pig (1)
Rabbit (1)
Sheep (1)
IHC (18)
IHC-P (5)
WB (25)
Flo (1)
ELISA (17)
IF (4)
IP (5)
Peptide-ELISA (3)
rabbit (26)
mouse (1)
goat (1)
Product Group
IHCPlus (3)
IgG (22)
IgG1 (1)
monoclonal mc (1)
polyclonal pc (27)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (17)
aa477-503 (7)
aa44-553 (4)
180-260 aa, Internal (1)
Internal (1)
Lys239 (1)
aa100-150 (1)
aa175-225 (1)
aa201-250 (1)
aa83-94 (1)
No (27)
Yes (1)
ATP5A1 / ATP Synthase Alpha Antibody - Anti-ATP5A1 / ATP Synthase Alpha antibody IHC staining of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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ATP5A1 / ATP Synthase Alpha Goat anti-Human Polyclonal (aa83-94) Antibody
Rabbit, Mouse, Sheep, Bovine, Rat, Hamster, Pig, Horse, Bat, Human, Monkey
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µg/$485
ATP5A1 / ATP Synthase Alpha Antibody - Anti-ATP5A1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa201-250) Antibody
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$440
ATP5A1 / ATP Synthase Alpha Antibody - Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, IHC-P, IP, WB
Unconjugated
50 µl/$460
ATP5A1 / ATP Synthase Alpha Antibody - Immunohistochemistry of paraffin-embedded human liver cancer using ATP5F1A Antibody at dilution of 1:100
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal Antibody
Mouse, Human
ELISA, IHC, IP, WB
Unconjugated
50 µg/$294; 100 µg/$360
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IF, IHC, IP, WB
Unconjugated
100 µg/$331
ATP5A1 / ATP Synthase Alpha Antibody - Western blot of ATP5A antibody
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (180-260 aa, Internal) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
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ATP5A1 / ATP Synthase Alpha Mouse anti-Human Monoclonal Antibody
Human
ELISA, Flo, IF, IHC, WB
Unconjugated
100 µg/$351
ATP5A1 / ATP Synthase Alpha Antibody - Staining 293 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
ATP5A1 / ATP Synthase Alpha Antibody - Detection of mouse ATP5A1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-ATP5A1 used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa175-225) Antibody
Mouse, Human
IHC, IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
ATP5A1 / ATP Synthase Alpha Antibody - Detection of mouse ATP5A1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-ATP5A1 used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa100-150) Antibody
Mouse, Human
IHC, IP, WB
Unconjugated
10 µl/$295; 100 µl/$514
ATP5A1 / ATP Synthase Alpha Antibody - ATP5A1 Antibody IHC of formalin-fixed and paraffin-embedded lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa477-503) Antibody
Human
IHC, IHC-P, WB
Unconjugated
400 µl/$393
ATP5A1 / ATP Synthase Alpha Antibody - Immunohistochemical analysis of ATP5A1 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Rat, Human
IHC, IHC-P, WB
Unconjugated
100 µl/$299
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (Lys239) Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$321; 100 µl/$403
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa477-503) Antibody
Human
IHC, WB
Unconjugated
200 µl/$734
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa477-503) (Biotin) Antibody
Human
ELISA, IHC, WB
Biotin Conjugated
200 µl/$952
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa477-503) (FITC) Antibody
Human
ELISA, IHC, WB
FITC Conjugated
200 µl/$952
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa477-503) (APC) Antibody
Human
ELISA, IHC, WB
APC Conjugated
200 µl/$952
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa477-503) (Azide-free) (HRP) Antibody
Human
ELISA, IHC, WB
HRP Conjugated, Azide-free
200 µl/$952
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa477-503) (PE) Antibody
Human
ELISA, IHC, WB
PE Conjugated
200 µl/$952
ATP5A1 / ATP Synthase Alpha Antibody - Western blot analysis of extracts from RAW264.7 cells and 293 cells, using ATP5A1 antibody.
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, WB
Unconjugated
100 µg/$357
ATP5A1 / ATP Synthase Alpha Antibody - Western blot. All lanes: ATP5A1 antibody at 2 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: COLO205 whole cell lysate. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 60 kDa. Observed band size: 60 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa44-553) Antibody
Human
ELISA, WB
Unconjugated
50 µg/$294; 100 µg/$360
ATP5A1 / ATP Synthase Alpha Antibody - Western blot. All lanes: ATP5A1 antibody at 2 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: COLO205 whole cell lysate. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 60 kDa. Observed band size: 60 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa44-553) (HRP) Antibody
Human
ELISA, WB
HRP Conjugated
50 µg/$294; 100 µg/$360
ATP5A1 / ATP Synthase Alpha Antibody - Western blot. All lanes: ATP5A1 antibody at 2 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: COLO205 whole cell lysate. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 60 kDa. Observed band size: 60 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa44-553) (Biotin) Antibody
Human
ELISA, WB
Biotin Conjugated
50 µg/$294; 100 µg/$360
ATP5A1 / ATP Synthase Alpha Antibody - Western blot. All lanes: ATP5A1 antibody at 2 ug/ml. Lane 1: HeLa whole cell lysate. Lane 2: COLO205 whole cell lysate. Secondary antibody: goat polyclonal to rabbit at 1:10000 dilution. Predicted band size: 60 kDa. Observed band size: 60 kDa.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal (aa44-553) (FITC) Antibody
Human
ELISA, WB
FITC Conjugated
50 µg/$294; 100 µg/$360
ATP5A1 / ATP Synthase Alpha Antibody - All lanes : Anti-ATP5A1 Antibody at 1:1000 dilution Lane 1: HepG2 whole cell lysates Lane 2: H.heart tissue lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/10000 dilution Predicted band size : 60 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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ATP5A1 / ATP Synthase Alpha Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
100 µl/$386
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).