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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ATG9A

autophagy related 9A

Involved in autophagy and cytoplasm to vacuole transport (Cvt) vesicle formation. Plays a key role in the organization of the preautophagosomal structure/phagophore assembly site (PAS), the nucleating site for formation of the sequestering vesicle. Cycles between a juxta-nuclear trans-Golgi network compartment and late endosomes. Nutrient starvation induces accumulation on autophagosomes. Starvation-dependent trafficking requires ULK1, ATG13 and SUPT20H.

Gene Name: autophagy related 9A
Synonyms: ATG9A, APG9-like 1, Autophagy 9-like 1 protein, Autophagy-related protein 9A, MGD3208, MATG9, APG9 autophagy 9-like 1, APG9L1, Autophagy related 9A
Target Sequences: NM_024085 NP_076990.4 Q7Z3C6

Publications (3)

1
Silica nanoconstruct cellular toleration threshold in vitro. Herd HL, Malugin A, Ghandehari H. Journal of controlled release : official journal of the Controlled Release Society. 2011 153:40-8. (WB; Mouse, Human) [PubMed:21342660] [PMC:PMC3197243]
2
Identification of autophagic vacuoles and regulators of autophagy in villous trophoblast from normal term pregnancies and in fetal growth restriction. Curtis S, Jones CJ, Garrod A, Hulme CH, Heazell AE. The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians. 2013 26:339-46. (IHC; Human) [PubMed:23039021]
3
Autophagy in Pancreatic Acinar Cells in Caerulein-treated Mice: Immunolocalization of Related Proteins and Their Potential as Markers of Pancreatitis. Zhang L, Zhang J, Shea K, Xu L, Tobin G, Knapton A, Sharron S, Rouse R. Toxicologic pathology. 2013 42:435-57. (ICC, WB; Human, Rat) [Full Text Article] [PubMed:23640381]

Filtered by: None
Target
ATG9A (35)
Reactivity
Human (33)
Mouse (14)
Rat (15)
Bovine (9)
Chicken (1)
Chimpanzee (6)
Dog (4)
Gibbon (3)
Guinea pig (4)
Horse (4)
Monkey (4)
Orangutan (6)
Pig (4)
Primate (1)
Rabbit (4)
Zebrafish (5)
Application
IHC (17)
IHC-P (7)
WB (26)
ELISA (17)
IF (4)
IP (1)
Peptide-ELISA (2)
Host
rabbit (35)
Isotype
Ig (1)
IgG (18)
Clonality
polyclonal pc (35)
Format
AP Conjugated (1)
APC Conjugated (1)
Azide-free (1)
Biotin Conjugated (3)
FITC Conjugated (3)
HRP Conjugated (3)
PE Conjugated (1)
Unconjugated (23)
Epitope
aa723-753 (6)
aa443-492 (4)
aa648-697 (4)
C-Terminus (3)
Ser738 (1)
aa 723-753 (1)
aa180-290 (1)
aa252-281 (1)
aa515-548 (1)
aa573-605 (1)
aa717-746 (1)
aa750-839 (1)
aa789-839 (1)
Publications
No (35)
Showing 1-25 of 35 results

IHC-plus™ ATG9A Antibody LS-B6984
Rabbit Polyclonal (IgG) to Human ATG9A
Mouse, Rat, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
50µg 1mg/ml  $545.00
Anti-ATG9A antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-ATG9A antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

ATG9A Antibody (C-Terminus) LS-C187451
Rabbit Polyclonal (IgG) to Human ATG9A
Human
ELISA, IHC, IHC-P, WB
Unconjugated
0.1mg  $375.00
Western blot of mouse heart tissue lysate probed with Rabbit anti-Human ATG9A

ATG9A Antibody (C-Terminus) LS-C74354
Rabbit Polyclonal to Human ATG9A
Rat, Human
ELISA, IF, IHC, WB
Unconjugated
100µl  $590.00

ATG9A Antibody (aa717-746) LS-C156622
Rabbit Polyclonal to Human ATG9A
Human
IF, IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Fluorescent image of U251 cells stained with ATG9A antibody. U251 cells were treated with Chloroquine (50 mu M,16h), then fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated ATG9A primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). ATG9A immunoreactivity is localized to autophagic vacuoles in the cytoplasm of U251 cells.

IHC-plus™ ATG9A Antibody (aa750-839) LS-B661
Rabbit Polyclonal to Human ATG9A
Chicken, Bovine, Rat, Mouse, Primate, Human
IHC, IHC-P, WB
Unconjugated
100µl  $425.00
Anti-ATG9A antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-ATG9A antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ATG9A Antibody LS-C404671
Rabbit Polyclonal (IgG) to Human ATG9A
Rat, Human
ELISA, IHC, WB
Unconjugated
20µl 0.6mg/ml  $205.00; 60µl 0.6mg/ml  $235.00; 120µl 0.6mg/ml  $295.00; 200µl 0.6mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human brain using ATG9A Polyclonal Antibody at dilution of 1:30.
Immunohistochemistry of paraffin-embedded Human thyroid cancer using ATG9A Polyclonal Antibody at dilution of 1:30.

ATG9A Antibody (aa252-281) LS-C156623
Rabbit Polyclonal to Human ATG9A
Mouse, Human
IHC, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
APG9L1 Antibody (W267) western blot of A2058 cell line lysates (35 ug/lane). The APG9L1 antibody detected the APG9L1 protein (arrow).

ATG9A Antibody (aa180-290) LS-C378005
Rabbit Polyclonal (IgG) to Human ATG9A
Human
ELISA, IHC, IHC-P
Unconjugated
50µl  $285.00; 100µl  $385.00
Immunohistochemistry of paraffin-embedded human breast cancer using antibody at 1:100 dilution.
Immunohistochemistry of paraffin-embedded human breast cancer using ATG9A Antibody at dilution of 1:100

ATG9A Antibody (Ser738) LS-C319673
Rabbit Polyclonal (IgG) to Human ATG9A
Human
ELISA, IHC
Unconjugated
200µl  $590.00

ATG9A Antibody (aa723-753, Biotin) LS-C212819
Rabbit Polyclonal (IgG) to Human ATG9A
Human
ELISA, IHC
Biotin Conjugated
200µl  $740.00

ATG9A Antibody (aa723-753, FITC) LS-C212822
Rabbit Polyclonal (IgG) to Human ATG9A
Human
ELISA, IHC
FITC Conjugated
200µl  $740.00

ATG9A Antibody (aa723-753, AP) LS-C212817
Rabbit Polyclonal (IgG) to Human ATG9A
Human
ELISA, IHC
AP Conjugated
200µl  $740.00

ATG9A Antibody (aa723-753, Azide-free, HRP) LS-C212823
Rabbit Polyclonal (IgG) to Human ATG9A
Human
ELISA, IHC
HRP Conjugated, Azide-free
200µl  $740.00

ATG9A Antibody (aa723-753, PE) LS-C212825
Rabbit Polyclonal (IgG) to Human ATG9A
Human
ELISA, IHC
PE Conjugated
200µl  $740.00

ATG9A Antibody (aa723-753, APC) LS-C212818
Rabbit Polyclonal (IgG) to Human ATG9A
Human
ELISA, IHC
APC Conjugated
200µl  $740.00

ATG9A Antibody LS-C746845
Rabbit Polyclonal (IgG) to Human ATG9A
Mouse, Rat, Human
IF, WB
Unconjugated
50µl  $235.00; 100µl  $295.00; 200µl  $385.00

ATG9A Antibody LS-C409538
Rabbit Polyclonal (IgG) to Human ATG9A
Mouse, Rat, Human
IHC, WB
Unconjugated
50µl  $255.00; 100µl  $315.00; 200µl  $425.00
Western blot analysis of extracts of HT-29 cells.

ATG9A Antibody LS-C401391
Rabbit Polyclonal (IgG) to Human ATG9A
Rat, Human
ELISA, IHC
Unconjugated
20µl 0.3mg/ml  $205.00; 60µl 0.3mg/ml  $235.00; 120µl 0.3mg/ml  $295.00; 200µl 0.3mg/ml  $395.00
Immunohistochemistry of paraffin-embedded Human brain using ATG9A Polyclonal Antibody at dilution of 1:40.
Immunohistochemistry of paraffin-embedded Human brain using ATG9A Polyclonal Antibody at dilution of 1:40.

ATG9A Antibody (aa 723-753) LS-C757554
Rabbit Polyclonal (Ig) to Human ATG9A
Human
ELISA, IHC-P, WB
Unconjugated
400µl  $325.00
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with APG9L1 Antibody (S738) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
All lanes: Anti-APG9L1 Antibody (S738) at 1:1000 dilution Lane 1: MCF-7 whole cell lysate Lane 2: HepG2 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 94kDa Blocking/Dilution buffer: 5% NFDM/TBST.

ATG9A Antibody LS-C764709
Rabbit Polyclonal (IgG) to Human ATG9A
Mouse, Rat, Human
ELISA, WB
Unconjugated
20µl 1mg/ml  $205.00; 60µl 1mg/ml  $235.00; 120µl 1mg/ml  $295.00; 200µl 1mg/ml  $395.00

ATG9A Antibody LS-C781215
Rabbit Polyclonal to Human ATG9A
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µg 100µl 1mg/ml  $305.00

ATG9A Antibody LS-C804250
Rabbit Polyclonal (IgG) to Human ATG9A
Mouse, Rat, Human
ELISA, WB
Unconjugated
100µl 1mg/ml  $325.00; 200µl 1mg/ml  $375.00
Western blot Image

ATG9A Antibody (aa515-548) LS-C356567
Rabbit Polyclonal to Human ATG9A
Mouse
Peptide-ELISA, WB
Unconjugated
400µl  $325.00
Anti-atg9a Antibody at 1:2000 dilution + mouse heart lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 96 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

ATG9A Antibody (aa573-605) LS-C392641
Rabbit Polyclonal to Human ATG9A
Zebrafish
Peptide-ELISA, WB
Unconjugated
400µl  $325.00
Anti-atg9a Antibody (C-Term)at 1:2000 dilution + ZF4 whole cell lysates Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 96 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

ATG9A Antibody (aa789-839) LS-C762739
Rabbit Polyclonal (IgG) to Human ATG9A
Human
IP, WB
Unconjugated
100µg 100µl 1mg/ml  $395.00
Detection of human ATG9A by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ATG9A antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Showing 1-25 of 35 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).