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Aspartate Aminotransferase

glutamic-oxaloacetic transaminase 1, soluble

Biosynthesis of L-glutamate from L-aspartate or L-cysteine. Important regulator of levels of glutamate, the major excitatory neurotransmitter of the vertebrate central nervous system. Acts as a scavenger of glutamate in brain neuroprotection. The aspartate aminotransferase activity is involved in hepatic glucose synthesis during development and in adipocyte glyceroneogenesis. Using L-cysteine as substrate, regulates levels of mercaptopyruvate, an important source of hydrogen sulfide. Mercaptopyruvate is converted into H2S via the action of 3-mercaptopyruvate sulfurtransferase (3MST). Hydrogen sulfide is an important synaptic modulator and neuroprotectant in the brain.

Gene Name: glutamic-oxaloacetic transaminase 1, soluble
Synonyms: GOT1, Aspartate Aminotransferase, ASTQTL1, Growth-inhibiting protein 18, Transaminase A, GIG18
Target Sequences: NM_002079 NP_002070.1 P17174

Publications (2)

1
CXCL14 and MCP1 are potent trophic factors associated with cell migration and angiogenesis leading to higher regenerative potential of dental pulp side population cells. Hayashi Y, Murakami M, Kawamura R, Ishizaka R, Fukuta O, Nakashima M. Stem cell research & therapy. 2015 6:111. (WB; Pig) [Full Text Article] [PubMed:26021377] [PMC:PMC4488059] Related Antibodies: LS-B24.
2
Stimulation of angiogenesis, neurogenesis and regeneration by side population cells from dental pulp. Ishizaka R, Hayashi Y, Iohara K, Sugiyama M, Murakami M, Yamamoto T, Fukuta O, Nakashima M. Biomaterials. 2013 Mar;34:1888-97. [Full Text Article] [PubMed:23245334] Related Antibodies: LS-B24.

☰ Filters
Products
Proteins (16)
Over-Expression Cell Culture Supernatant (1)
Over-Expression Lysate (3)
Recombinant (12)
Aspartate Aminotransferase (16)
No (16)
6His, C-terminus (2)
GST (1)
His (6)
His, N-Terminal (3)
His-T7 (1)
Myc-DDK (Flag) (3)
Bovine (1)
Human (11)
Mouse (2)
Rat (1)
293T Cells (1)
E. coli (8)
Eukaryotic cells (1)
HEK 293 Cells (4)
Wheat Germ Extract (1)
Yeast (1)
Purified (2)
Low endotoxin level (2)
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HEK 293 Cells
His, N-Terminal
~46 kDa
200 µl/$283
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HEK 293 Cells
His, N-Terminal
~46 kDa
100 µg/$298
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E. coli
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
47.3 kDa
10 µg/$376; 50 µg/$526
Aspartate Aminotransferase Protein - Recombinant Aspartate Aminotransferase By SDS-PAGE
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E. coli
His-T7
50.0 kDa
10 µg/$287; 50 µg/$482; 100 µg/$770; 200 µg/$962; 1 mg/$2,094; 500 µg/$1,623; 5 mg/$3,361; 2 mg/$2,284
Aspartate Aminotransferase Protein - 12.5% SDS-PAGE of human GOT1 stained with Coomassie Blue
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Wheat Germ Extract
GST
72.6 kDa
10 µg/$479; 25 µg/$670
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293T Cells
Myc-DDK (Flag)
46.1 kDa
20 µg/$150
Aspartate Aminotransferase Protein - Purified recombinant protein GOT1 was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
46.1 kDa
20 µg/$1,107
Aspartate Aminotransferase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
46.1 kDa
100 µg/$494
Aspartate Aminotransferase Protein
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Yeast
His
47.5 kDa
10 µg/$313; 50 µg/$512; 100 µg/$767
Aspartate Aminotransferase Protein - Recombinant Aspartate Aminotransferase By SDS-PAGE
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E. coli
His
47.5 kDa
10 µg/$287; 50 µg/$482; 100 µg/$770; 200 µg/$962; 1 mg/$2,094; 500 µg/$1,623; 5 mg/$3,361; 2 mg/$2,284
Select
E. coli
His
47.7 kDa
10 µg/$287; 50 µg/$482; 100 µg/$770; 200 µg/$962; 1 mg/$2,094; 500 µg/$1,623; 5 mg/$3,361; 2 mg/$2,284
Aspartate Aminotransferase Protein - Recombinant Aspartate Aminotransferase By SDS-PAGE
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E. coli
His
47.8 kDa
10 µg/$278; 50 µg/$422; 100 µg/$672; 200 µg/$842; 1 mg/$1,934; 500 µg/$1,480; 5 mg/$2,941; 2 mg/$2,119
Aspartate Aminotransferase Protein
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E. coli
His
100 µg/$361; 500 µg/$739; 20 µg/$261
Aspartate Aminotransferase Protein
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E. coli
His
100 µg/$342; 500 µg/$641; 20 µg/$254
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E. coli
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
47.31kD
10 µg/$288; 50 µg/$442; 500 µg/$1,585; 1 mg/$2,157
Aspartate Aminotransferase Protein - Eukaryotic Aspartate Aminotransferase (AST) by SDS-PAGE
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Eukaryotic cells
His, N-Terminal
48kDa
10 µg/$292; 50 µg/$512; 200 µg/$1,018; 100 µg/$817; 1 mg/$2,168; 500 µg/$1,690; 5 mg/$3,571; 2 mg/$2,359
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).