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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ASAP2 / DDEF2

ArfGAP with SH3 domain, ankyrin repeat and PH domain 2

ASAP2 / DDEF2 is a multidomain protein containing an N-terminal alpha-helical region with a coiled-coil motif, followed by a pleckstrin homology (PH) domain, an Arf-GAP domain, an ankyrin homology region, a proline-rich region, and a C-terminal Src homology 3 (SH3) domain. The protein localizes in the Golgi apparatus and at the plasma membrane, where it colocalizes with protein tyrosine kinase 2-beta (PYK2). The encoded protein forms a stable complex with PYK2 in vivo. This interaction appears to be mediated by binding of its SH3 domain to the C-terminal proline-rich domain of PYK2. The encoded protein is tyrosine phosphorylated by activated PYK2. It has catalytic activity for class I and II ArfGAPs in vitro, and can bind the class III Arf ARF6 without immediate GAP activity. The encoded protein is believed to function as an ARF GAP that controls ARF-mediated vesicle budding when recruited to Golgi membranes. In addition, it functions as a substrate and downstream target for PYK2 and SRC, a pathway that may be involved in the regulation of vesicular transport. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Name: ArfGAP with SH3 domain, ankyrin repeat and PH domain 2
Synonyms: ASAP2, AMAP2, Centaurin, beta 3, DDEF2, CENTB3, PAP, KIAA0400, SHAG1, PAG3, PAP-alpha
Target Sequences: NM_003887 NP_003878.1 O43150

Filtered By: None
Target
ASAP2 / DDEF2 (10)
Reactivity
Human (10)
Mouse (4)
Rat (3)
Dog (4)
Horse (1)
Monkey (1)
Opossum (1)
Rabbit (4)
Application
IHC (1)
IHC-P (1)
WB (10)
IP (1)
Host
rabbit (10)
Isotype
IgG (1)
Clonality
polyclonal pc (10)
Format
Biotin Conjugated (1)
FITC Conjugated (1)
HRP Conjugated (1)
Unconjugated (7)
Epitope
N-Terminus (4)
Internal (1)
aa500-760 (1)
aa700-750 (1)
aa900-950 (1)
pTyr724 (1)
Publications
No (10)
Showing 1-10 of 10 results

ASAP2 / DDEF2 Antibody (Internal) IHC-plus™ LS-B13654
Rabbit Polyclonal to Human ASAP2 / DDEF2
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µl 1mg/ml  $425.00
Human Skeletal Muscle, Nerve: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Skeletal Muscle, Nerve: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE)

ASAP2 / DDEF2 Antibody (aa700-750) LS-C148569
Rabbit Polyclonal to Human ASAP2 / DDEF2
Mouse, Rat, Opossum, Human, Monkey
WB
Unconjugated
100µg 0.5mg/ml  $335.00
Western blot of ASAP2 in human brain lysate using 1) preimmune sera at 1:5000 and 2) protein A purified Polyclonal Antibody to ASAP2 at 1.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.
Western blot of ASAP2 in human brain lysate using 1) preimmune sera at 1:5000 and 2) protein A purified Polyclonal Antibody to ASAP2 at 1.0 ug/ml. Goat anti-rabbit Ig HRP secondary antibody, and PicoTect ECL substrate solution, were used for this test.

ASAP2 / DDEF2 Antibody (aa900-950) LS-C289997
Rabbit Polyclonal (IgG) to Human ASAP2 / DDEF2
Horse, Human
IP WB
Unconjugated
100µl 1mg/ml  $395.00
Detection of Human ASAP2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ASAP2 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ASAP2 was also immunoprecipitated by rabbit anti-ASAP2 antibody BL11866, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Detection of Human ASAP2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-ASAP2 antibody used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 6 ug/mg lysate. ASAP2 was also immunoprecipitated by rabbit anti-ASAP2 antibody BL11866, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).

ASAP2 / DDEF2 Antibody (phospho-Tyr724) LS-C38032
Rabbit Polyclonal to Human ASAP2 / DDEF2
Mouse, Human
WB
Unconjugated
10blot  $635.00

ASAP2 / DDEF2 Antibody (aa500-760) LS-C358218
Rabbit Polyclonal to Human ASAP2 / DDEF2
Human
WB
Unconjugated
200µl 0.5mg/ml  $315.00

ASAP2 / DDEF2 Antibody LS-C411900
Rabbit Polyclonal to Human ASAP2 / DDEF2
Mouse, Rat, Human
WB
Unconjugated
100µl 1mg/ml  $385.00
Western blot of ASAP2 antibody at 1:500 dilution. Lane 1: HEK293T whole cell lysate. Lane 2: sp2/0 whole cell lysate. Lane 3: H9C2 whole cell lysate.
Western blot of ASAP2 antibody at 1:500 dilution. Lane 1: HEK293T whole cell lysate. Lane 2: sp2/0 whole cell lysate. Lane 3: H9C2 whole cell lysate.

ASAP2 / DDEF2 Antibody (N-Terminus) LS-C345175
Rabbit Polyclonal to Human ASAP2 / DDEF2
Rabbit, Dog, Human
WB
Unconjugated
50µg  $365.00
Western blot of ASAP2 Antibody - N-terminal region with human MCF7 cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of ASAP2 Antibody - N-terminal region with human MCF7 cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ASAP2 / DDEF2 Antibody (N-Terminus, HRP) LS-C475518
Rabbit Polyclonal to Human ASAP2 / DDEF2
Rabbit, Dog, Human
WB
HRP Conjugated
100µl 0.65mg/ml  $385.00
Western blot of ASAP2 Antibody - N-terminal region with human MCF7 cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of ASAP2 Antibody - N-terminal region with human MCF7 cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ASAP2 / DDEF2 Antibody (N-Terminus, Biotin) LS-C475516
Rabbit Polyclonal to Human ASAP2 / DDEF2
Rabbit, Dog, Human
WB
Biotin Conjugated
100µl 1mg/ml  $385.00
Western blot of ASAP2 Antibody - N-terminal region with human MCF7 cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of ASAP2 Antibody - N-terminal region with human MCF7 cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ASAP2 / DDEF2 Antibody (N-Terminus, FITC) LS-C475517
Rabbit Polyclonal to Human ASAP2 / DDEF2
Rabbit, Dog, Human
WB
FITC Conjugated
100µl 1mg/ml  $385.00
Western blot of ASAP2 Antibody - N-terminal region with human MCF7 cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of ASAP2 Antibody - N-terminal region with human MCF7 cells lysate.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Showing 1-10 of 10 results

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If you do not find the reagent or information you require, please contact customer.support@lsbio.com to inquire about additional products in development.

PLEASE NOTE

For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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