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APOB / Apolipoprotein B

apolipoprotein B

Apolipoprotein B (ApoB) is the main apolipoprotein of chylomicrons and low density lipoproteins (LDL). The protein occurs in the plasma in 2 main isoforms, apoB-48 and apoB-100. The first is synthesized exclusively by the gut, the second by the liver. The intestinal (B-48) and hepatic (B-100) forms of apoB are coded by a single gene and by a single mRNA transcript larger than 16 kb. The 2 proteins share a common amino terminal sequence. In the ApoB-100 isoform the precursor has 4563 amino acids, and the mature apoB-100 has 4536 amino acid residues. Mature, circulating B-48 is homologous over its entire length (estimated to be between 2130 and 2144 amino acid residues) with the amino-terminal portion of B-100 and contains no sequence from the carboxyl end of B-100. From structural studies, it is thought that apoB-48 represents the amino-terminal 47% of apoB-100 and that the carboxyl terminus of apoB-48 is in the vicinity of residue 2151 of apoB-100. Apolipoprotein B-48 may be the product of an intestinal mRNA with an in-frame UAA stop codon resulting from a C-to-U change in the codon CAA encoding Gln(2153) in apoB-100 mRNA. Since only the sequence that codes B-100 is present in genomic DNA, this presents the possibility of an organ-specific introduction of a stop codon to an mRNA and the change from CAA to UAA of codon 2153 of the message as a unique RNA editing process.

Gene Name: apolipoprotein B
Synonyms: APOB, ApoB-100, Apolipoprotein B-48, ApoB-48, Apolipoprotein B, FLDB, LDLCQ4, Mutant Apo B 100, Apo B-100, Apolipoprotein B-100, Apolipoprotein B48
Target Sequences: NM_000384 NP_000375.2 P04114

Publications (7)

1
Apolipoprotein B48, the Structural Component of Chylomicrons, Is Sufficient to Antagonize Staphylococcus aureus Quorum-Sensing. Elmore BO, Triplett KD, Hall PR. PloS one. 2015 10:e0125027. (WB; Mouse) [Full Text Article] [PubMed:25942561] [PMC:PMC4420250] Related Antibodies: LS-B6180.
2
Hepatocyte estrogen receptor alpha mediates estrogen action to promote reverse cholesterol transport during Western-type diet feeding. Zhu L, Shi J, Luu TN, Neuman JC, Trefts E, Yu S, Palmisano BT, Wasserman DH, Linton MF, Stafford JM. Molecular metabolism. 2018 February;8:106-116. [Full Text Article] [PubMed:29331506] [PMC:PMC5985047] Related Antibodies: LS-B6180.
3
Spatial distributions of lipids in atherosclerosis of human coronary arteries studied by time-of-flight secondary ion mass spectrometry. Lehti S, Sjövall P, Käkelä R, Mäyränpää MI, Kovanen PT, Öörni K. The American journal of pathology. 2015 May;185:1216-1233. [Full Text Article] [PubMed:25805604]
4
Extracellular Lipids Accumulate in Human Carotid Arteries as Distinct Three-Dimensional Structures and Have Proinflammatory Properties. Satu Lehti, Su D Nguyen, Ilya Belevich, Helena Vihinen, Hanna M Heikkilä, Rabah Soliymani, Reijo Käkelä, Jani Saksi, Matti Jauhiainen, Gregory A Grabowski, Outi Kummu, Sohvi Hörkkö, Marc Baumann, Perttu J Lindsberg, Eija Jokitalo, Petri T Kovanen, Katariina Öörni. The American journal of pathology. 2018 Feb;188:525-538. [Full Text Article] [PubMed:29154769]
5
Estrogen-related receptor ? regulates hepatic triglyceride metabolism through phospholipase A2 G12B. Longhui Chen, Min Wu, Shengnan Zhang, Wenjuan Tan, Min Guan, Liqiang Feng, Chen Chen, Jun Tao, Ling Chen, Linbing Qu. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 2019 Jul;33:7942-7952. [Full Text Article] [PubMed:30922124]
6
Estrogen signaling prevents diet-induced hepatic insulin resistance in male mice with obesity. Zhu L, Martinez MN, Emfinger CH, Palmisano BT, Stafford JM. American journal of physiology. Endocrinology and metabolism. 2014 May;306:E1188-97. [Full Text Article] [PubMed:24691030] [PMC:PMC4116406] Related Antibodies: LS-B6180.
7
Estrogen treatment after ovariectomy protects against fatty liver and may improve pathway-selective insulin resistance. Zhu L, Brown WC, Cai Q, Krust A, Chambon P, McGuinness OP, Stafford JM. Diabetes. 2013 Feb;62:424-34. [Full Text Article] [PubMed:22966069] [PMC:PMC3554377] Related Antibodies: LS-B6180.
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APOB / Apolipoprotein B (39)
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APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Microplate
Human
78.13 - 5000 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$626
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Microplate
Mouse
31.25 - 2000 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$587
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Strip Plate
Rat
6.25 - 400 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$893
APOB / Apolipoprotein B ELISA Kit
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Competitive EIA
96-Well Microplate
Mouse
0.156 - 10 µg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$734
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Strip Plate
Mouse
6.25 - 400 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$883
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Strip Plate
Mouse
6.25 - 400 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$854
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Microplate
Human
2.458 - 600 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Plasma, Serum
1 Plate/$700
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Strip Plate
Bovine
18.75 - 1200 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$889
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Strip Plate
Pig
0.625 - 40 µg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$579
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Competitive EIA
96-Well Microplate
Chicken
0.156 - 10 µg/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$734
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Strip Plate
Rat
3.12 - 200 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum, Tissue Homogenates
1 Plate/$579
APOB / Apolipoprotein B ELISA Kit
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Development Kit
Antibody Pair and Standard
Rat
12.5 - 800 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
500 Wells/$1,356; 1000 Wells/$2,037
APOB / Apolipoprotein B ELISA Kit
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Custom
96-Well Strip Plate
Chicken
Colorimetric - 450nm (TMB)
1 Plate/$929
APOB / Apolipoprotein B ELISA Kit
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Custom
96-Well Strip Plate
Mouse
Colorimetric - 450nm (TMB)
1 Plate/$783
APOB / Apolipoprotein B ELISA Kit
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Sandwich CLIA
12 x 8-Well Microstrips
Human
15.625 - 1000 ng/ml
Chemiluminescent
Plasma, Serum
1 Plate/$728
APOB / Apolipoprotein B ELISA Kit
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Sandwich CLIA
12 x 8-Well Microstrips
Mouse
6.25 - 400 ng/ml
Chemiluminescent
Plasma, Serum
1 Plate/$728
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Microplate
Rat
31.25 - 2000 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$626
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Strip Plate
Rat
12.5 - 800 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
1 Plate/$741
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Strip Plate
Human
23.44 - 1500 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$600
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Microplate
Pig
62.5 - 4000 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$627
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Strip Plate
Human
1.56 - 100 ng/ml
Colorimetric - 450nm (TMB)
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
1 Plate/$749
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Strip Plate
Sheep
39.063 - 2500 ng/ml
Colorimetric - 450nm (TMB)
1 Plate/$760
APOB / Apolipoprotein B ELISA Kit
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Sandwich
96-Well Strip Plate
Rabbit
0.313 - 20 ng/ml
Colorimetric - 450nm (TMB)
1 Plate/$627
APOB / Apolipoprotein B ELISA Kit
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Sandwich CLIA
96-Well Strip Plate
Human
6.86 - 5000 ng/ml
Chemiluminescent
Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Homogenates
1 Plate/$968
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Sandwich
96-Well Strip Plate
Rat
50 - 1000 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$913
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).