AP50 / AP2M1

adaptor-related protein complex 2, mu 1 subunit

Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. The AP-2 mu subunit binds to transmembrane cargo proteins; it recognizes the Y-X-X-Phi motifs. The surface region interacting with to the Y-X-X-Phi motif is inaccessible in cytosolic AP-2, but becomes accessible through a conformational change following phosphorylation of AP-2 mu subunit at 'Tyr-156' in membrane-associated AP-2. The membrane-specific phosphorylation event appears to involve assembled clathrin which activates the AP-2 mu kinase AAK1 (By similarity). Plays a role in endocytosis of frizzled family members upon Wnt signaling.

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Products

Proteins (10)

Type

Over-Expression Lysate (4)

Recombinant (6)

Target

AP50 / AP2M1 (10)

Publications

No (10)

Tag

GST (2)

His (1)

His-S (1)

His-SUMO (1)

Myc-DDK (Flag) (5)

Species

Human (8)

Source

293T Cells (2)

E. coli (3)

HEK 293 Cells (3)

Wheat Germ Extract (1)

Yeast (1)

Purification

Purified (2)

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Human AP50 / AP2M1 Protein (Recombinant His-SUMO) (Full Length) - LS-G20482

Source:

E. coli

Tag:

His-SUMO

Molecular Weight:

65.6 kD

Price:

1 mg/$1,355; 100 µg/$420; 20 µg/$323

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Human AP50 / AP2M1 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G65262

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

49.2 kDa

Price:

20 µg/$1,107

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AP50 / AP2M1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G116891

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

49.5 kDa

Price:

100 µg/$494

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AP50 / AP2M1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G121026

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

49.2 kDa

Price:

100 µg/$494

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Human AP50 / AP2M1 Protein (Recombinant His) (Full Length) - LS-G22799

Source:

Yeast

Tag:

His

Molecular Weight:

51.6 kD

Price:

1 mg/$1,973; 100 µg/$570; 20 µg/$395

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Human AP50 / AP2M1 Protein (Recombinant GST) (Full Length) - LS-G22066

Source:

E. coli

Tag:

GST

Molecular Weight:

77 kD

Price:

100 µg/$420; 20 µg/$323; 1 mg/$1,355

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Human AP50 / AP2M1 Protein (Recombinant His-S) (aa170-435) - LS-G25687

Source:

E. coli

Tag:

His-S

Molecular Weight:

37.6 kDa

Price:

10 µg/$281; 50 µg/$442; 100 µg/$705; 200 µg/$882; 1 mg/$1,989; 500 µg/$1,529; 5 mg/$3,081; 2 mg/$2,176

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Human AP50 / AP2M1 Protein (Recombinant GST) (Full Length) - LS-G27937

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

76.1 kDa

Price:

2 µg/$439

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Human AP50 / AP2M1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G75193

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

49.2 kDa

Price:

20 µg/$150

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Human AP50 / AP2M1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G75195

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

49.5 kDa

Price:

20 µg/$150

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