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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ANGPT1 / Angiopoietin-1

angiopoietin 1

Binds and activates TEK/TIE2 receptor by inducing its dimerization and tyrosine phosphorylation. Plays an important role in the regulation of angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Required for normal angiogenesis and heart development during embryogenesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. Mediates blood vessel maturation/stability. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme.

Gene Name: angiopoietin 1
Synonyms: ANGPT1, Angiopoietin 1, Angiopoietin-1, AGPT, AGP1, ANG-1, ANG1, KIAA0003
Target Sequences: NM_001146 NP_001137.2 Q15389

Filtered By: None
Target
ANGPT1 / Angiopoietin-1 (139)
Reactivity
Human (92)
Mouse (74)
Rat (42)
Bat (4)
Bovine (7)
Chicken (4)
Chimpanzee (7)
Dog (23)
Gibbon (7)
Horse (5)
Monkey (5)
Orangutan (1)
Pig (7)
Rabbit (5)
Xenopus (4)
Application
IHC (60)
IHC-P (8)
WB (137)
ELISA (28)
ICC (1)
IF (1)
IP (2)
Peptide-ELISA (2)
Host
rabbit (119)
mouse (19)
goat (1)
Isotype
IgG (95)
IgG1 (2)
Clonality
monoclonal mc (20)
polyclonal pc (119)
Clone
7F9 (1)
8H2 (1)
Format
AP Conjugated (2)
APC (9)
APC Conjugated (11)
Azide-free (1)
Biotin Conjugated (15)
Cy3 Conjugated (9)
Cy7 Conjugated (9)
FITC Conjugated (18)
HRP Conjugated (16)
Magnetic beads Conjugated (1)
PE Conjugated (11)
Sepharose Conjugated (1)
Unconjugated (46)
Epitope
aa304-450 (10)
aa253-429 (9)
aa266-497 (9)
aa284-452 (9)
N-Terminus (8)
aa148-266 (8)
aa21-228 (8)
aa35-231 (8)
aa374-402 (8)
aa360-409 (4)
aa278-295 (3)
Internal (2)
aa198-498 (2)
C-Terminus (1)
N-Terminal (1)
aa158-207 (1)
aa16-350 (1)
aa176-224 (1)
aa225-239 (1)
aa76-85 (1)
Publications
No (139)
Showing 1-25 of 139 results

ANGPT1 / Angiopoietin-1 Antibody (N-Terminus) IHC-plus™ LS-B62
Rabbit Polyclonal to Mouse ANGPT1 / Angiopoietin-1
Mouse, Human
ELISA IHC IHC-P WB
Unconjugated
50µl 80mg/ml  $425.00
Anti-ANGPT1 / Angiopoietin-1 antibody IHC staining of mouse liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-ANGPT1 / Angiopoietin-1 antibody IHC staining of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-ANGPT1 / Angiopoietin-1 antibody IHC staining of mouse liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
Anti-ANGPT1 / Angiopoietin-1 antibody IHC staining of mouse kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.

ANGPT1 / Angiopoietin-1 Antibody (aa76-85) IHC-plus™ LS-B6867
Goat Polyclonal to Human ANGPT1 / Angiopoietin-1
Rabbit, Mouse, Dog, Horse, Human, Monkey
IHC IHC-P Peptide-ELISA WB
Unconjugated
50µg 0.5mg/ml  $425.00
Anti-ANGPT1 / Angiopoietin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human ANGPT1 with DYKDDDDK tag probed with ANGPT1 antibody (1 ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with ANGPT1 antibody (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.
Anti-ANGPT1 / Angiopoietin-1 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human ANGPT1 with DYKDDDDK tag probed with ANGPT1 antibody (1 ug/ml) in Lane A and probed with anti-DYKDDDDK Tag (1/1000) in lane C. Mock-transfected HEK293 probed with ANGPT1 antibody (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

ANGPT1 / Angiopoietin-1 Antibody (aa278-295) LS-C172153
Rabbit Polyclonal to Human ANGPT1 / Angiopoietin-1
Human
IHC IHC-P WB
Unconjugated
100µg 1mg/ml  $295.00
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
 This image was taken for the unconjugated form of this product. Other forms have not been tested.
 This image was taken for the unconjugated form of this product. Other forms have not been tested.

ANGPT1 / Angiopoietin-1 Antibody (aa360-409) LS-C151507
Rabbit Polyclonal to Mouse ANGPT1 / Angiopoietin-1
Xenopus, Chicken, Bovine, Rabbit, Mouse, Dog, Rat, Pig, Horse, Bat, Human, Monkey
WB
Unconjugated
50µg  $365.00
ANGPT1 / ANG1 / Angiopoietin-1 antibody Western Blot of Mouse Liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
ANGPT1 / ANG1 / Angiopoietin-1 antibody Western Blot of Mouse Liver.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ANGPT1 / Angiopoietin-1 Antibody (aa225-239) LS-C343878
Rabbit Polyclonal to Human ANGPT1 / Angiopoietin-1
Mouse, Bovine, Rat, Pig, Orangutan, Gibbon, Chimpanzee, Human
ELISA IHC IHC-P WB
Unconjugated
100µg 200µl 0.5mg/ml  $355.00
Angiopoietin 1 antibody IHC-paraffin: Human Lung Cancer Tissue.
Angiopoietin 1 antibody Western blot. Lane 1: Rat Heart Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Kidney Tissue Lysate. Lane 4: SMMC Cell Lysate.
Angiopoietin 1 antibody IHC-paraffin: Human Lung Cancer Tissue.
Angiopoietin 1 antibody Western blot. Lane 1: Rat Heart Tissue Lysate. Lane 2: Rat Brain Tissue Lysate. Lane 3: Rat Kidney Tissue Lysate. Lane 4: SMMC Cell Lysate.

ANGPT1 / Angiopoietin-1 Antibody (aa176-224) IHC-plus™ LS-B10907
Rabbit Polyclonal to Human ANGPT1 / Angiopoietin-1
Mouse, Rat, Human
IHC IHC-P WB
Unconjugated
50µl  $425.00
Anti-Angiogenin / ANG antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot of p-CD318 (Y707) pAb in extracts from HeLa cells.
Anti-Angiogenin / ANG antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
Western blot of p-CD318 (Y707) pAb in extracts from HeLa cells.

ANGPT1 / Angiopoietin-1 Antibody (N-Terminus) LS-C137047
Rabbit Polyclonal (IgG) to Human ANGPT1 / Angiopoietin-1
Mouse, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $255.00
Western blot Image
Western blot Image

ANGPT1 / Angiopoietin-1 Antibody (aa304-450) LS-C292803
Rabbit Polyclonal (IgG) to Rat ANGPT1 / Angiopoietin-1
Rat
IHC WB
Unconjugated
20µl 0.5mg/ml  $205.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $295.00; 1ml 0.5mg/ml  $515.00; 10ml 0.5mg/ml  $2,040.00
Western blot of recombinant ANGPT1 / ANG1 / Angiopoietin-1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant ANGPT1 / ANG1 / Angiopoietin-1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ANGPT1 / Angiopoietin-1 Antibody (aa374-402, FITC) LS-C251294
Rabbit Polyclonal (IgG) to Human ANGPT1 / Angiopoietin-1
Human
ELISA WB
FITC Conjugated
200µl 0.5mg/ml  $680.00

ANGPT1 / Angiopoietin-1 Antibody (aa374-402, Azide-free, HRP) LS-C251296
Rabbit Polyclonal (IgG) to Human ANGPT1 / Angiopoietin-1
Human
ELISA WB
HRP Conjugated, Azide-free
200µl 0.45mg/ml  $680.00

ANGPT1 / Angiopoietin-1 Antibody (aa158-207) IHC-plus™ LS-B13444
Rabbit Polyclonal (IgG) to Human ANGPT1 / Angiopoietin-1
Mouse, Rat, Human
IHC IHC-P Peptide-ELISA WB
Unconjugated
100µl 1mg/ml  $505.00
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of Ang-1 antibody in extracts from HeLa cells.
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of Ang-1 antibody in extracts from HeLa cells.

ANGPT1 / Angiopoietin-1 Antibody (C-Terminus) LS-C347655
Rabbit Polyclonal (IgG) to Human ANGPT1 / Angiopoietin-1
Human
WB
Unconjugated
100µl  $405.00

ANGPT1 / Angiopoietin-1 Antibody LS-C748039
Rabbit Monoclonal (IgG) to Human ANGPT1 / Angiopoietin-1
Human
WB
Unconjugated
50µl  $265.00

ANGPT1 / Angiopoietin-1 Antibody IHC-plus™ LS-B14394
Rabbit Polyclonal (IgG) to Human ANGPT1 / Angiopoietin-1
Mouse, Human
IHC IHC-P WB
Unconjugated
50µl  $425.00
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of extracts of various cell lines, using ANGPT1 antibody.
Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
Western blot of extracts of various cell lines, using ANGPT1 antibody.

ANGPT1 / Angiopoietin-1 Antibody (N-Terminal) LS-C387136
Rabbit Polyclonal (IgG) to Human ANGPT1 / Angiopoietin-1
Rat, Human
ELISA IHC WB
Unconjugated
100µl 1mg/ml  $265.00
Immunohistochemistry of Ang-1 antibody
Immunohistochemistry of Ang-1 antibody
Immunohistochemistry of Ang-1 antibody
Immunohistochemistry of Ang-1 antibody

ANGPT1 / Angiopoietin-1 Antibody LS-C86954
Rabbit Polyclonal to Mouse ANGPT1 / Angiopoietin-1
Mouse
ELISA IHC WB
Unconjugated
100µg  $665.00

ANGPT1 / Angiopoietin-1 Antibody LS-C744593
Rabbit Polyclonal to Mouse ANGPT1 / Angiopoietin-1
Mouse, Human
ELISA IHC WB
Unconjugated
25µl  $235.00
Anti-ANG1 antibody was diluted 1:500 to detect ANG1 in human lung tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Rabbit anti-ANG1 was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells. Approximately 20 µg of each lysate was used for 10% SDS-PAGE. Immunoprecipitation preceded the reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development.
Anti-ANG1 antibody was diluted 1:500 to detect ANG1 in human lung tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Rabbit anti-ANG1 was used at a 1:500 dilution to detect mouse Ang-1 by western blot against supernatants of mouse angiopoietin-expressing endothelial cells. Lane 1 - wt endothelial cells. Lane 2 - mouse Ang-1 (clone 1-8) expressing cells. Lane 3 - mouse Ang-1 (clone 1-15) expressing cells. Lane 4 - mouse Ang-2 (clone 2-9) expressing cells. Approximately 20 µg of each lysate was used for 10% SDS-PAGE. Immunoprecipitation preceded the reaction with primary antibody at room temperature for 1 h. After subsequent washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development.

ANGPT1 / Angiopoietin-1 Antibody (Internal) LS-C368576
Rabbit Polyclonal to Human ANGPT1 / Angiopoietin-1
Mouse, Bovine, Rat, Pig, Human
ICC IF WB
Unconjugated
30µl 1mg/ml  $205.00; 100µl 1mg/ml  $245.00; 200µl 1mg/ml  $305.00
Immunofluorescent analysis of Angiopoietin-1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Angiopoietin-1 expression in MCF7 (A); HeLa (B); U87MG (C) whole cell lysates.
Immunofluorescent analysis of Angiopoietin-1 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Angiopoietin-1 expression in MCF7 (A); HeLa (B); U87MG (C) whole cell lysates.

ANGPT1 / Angiopoietin-1 Antibody LS-C659706
Rabbit Polyclonal (IgG) to Human ANGPT1 / Angiopoietin-1
Human
ELISA IHC WB
Unconjugated
100µg  $435.00

ANGPT1 / Angiopoietin-1 Antibody (aa198-498, clone 8H2) LS-C339272
Mouse Monoclonal [clone 8H2] (IgG1) to Human ANGPT1 / Angiopoietin-1
Human
IHC IHC-P WB
Unconjugated
100µl 1mg/ml  $335.00
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-ANGPT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Human recombinant protein fragment corresponding to amino acids 198-498 of human ANGPT1 (NP_001137) produced in E.coli.
IHC of paraffin-embedded Carcinoma of Human liver tissue using anti-ANGPT1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Human recombinant protein fragment corresponding to amino acids 198-498 of human ANGPT1 (NP_001137) produced in E.coli.

ANGPT1 / Angiopoietin-1 Antibody LS-C409426
Rabbit Polyclonal (IgG) to Human ANGPT1 / Angiopoietin-1
Mouse, Rat, Human
IHC WB
Unconjugated
50µl  $255.00; 100µl  $315.00; 200µl  $425.00
Immunohistochemistry of paraffin-embedded rat lung tissue.
Immunohistochemistry of paraffin-embedded human esophagus.
Immunohistochemistry of paraffin-embedded rat lung tissue.
Immunohistochemistry of paraffin-embedded human esophagus.

ANGPT1 / Angiopoietin-1 Antibody (aa253-429) LS-C292804
Rabbit Polyclonal (IgG) to Mouse ANGPT1 / Angiopoietin-1
Mouse
IHC WB
Unconjugated
20µl 0.5mg/ml  $195.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $285.00; 1ml 0.5mg/ml  $485.00; 10ml 0.5mg/ml  $1,935.00
Western blot of recombinant ANGPT1 / ANG1 / Angiopoietin-1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant ANGPT1 / ANG1 / Angiopoietin-1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ANGPT1 / Angiopoietin-1 Antibody (aa35-231) LS-C292806
Rabbit Polyclonal (IgG) to Mouse ANGPT1 / Angiopoietin-1
Mouse
IHC WB
Unconjugated
20µl 0.5mg/ml  $195.00; 100µl 0.5mg/ml  $255.00; 200µl 0.5mg/ml  $285.00; 1ml 0.5mg/ml  $485.00; 10ml 0.5mg/ml  $1,935.00
Western blot of recombinant ANGPT1 / ANG1 / Angiopoietin-1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot of recombinant ANGPT1 / ANG1 / Angiopoietin-1.  This image was taken for the unconjugated form of this product. Other forms have not been tested.

ANGPT1 / Angiopoietin-1 Antibody (HRP) LS-C745017
Rabbit Polyclonal (IgG) to Human ANGPT1 / Angiopoietin-1
Human
ELISA IHC WB
HRP Conjugated
25µl  $235.00

ANGPT1 / Angiopoietin-1 Antibody (HRP) LS-C745020
Rabbit Polyclonal (IgG) to Human ANGPT1 / Angiopoietin-1
Human
ELISA IHC WB
HRP Conjugated
100µg  $425.00
Showing 1-25 of 139 results

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For RESEARCH USE ONLY. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).

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