AKT1

v-akt murine thymoma viral oncogene homolog 1

AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) called the AKT kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. This is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Over 100 substrate candidates have been reported so far, but for most of them, no isoform specificity has been reported. AKT is responsible of the regulation of glucose uptake by mediating insulin-induced translocation of the SLC2A4/GLUT4 glucose transporter to the cell surface. Phosphorylation of PTPN1 at 'Ser-50' negatively modulates its phosphatase activity preventing dephosphorylation of the insulin receptor and the attenuation of insulin signaling. Phosphorylation of TBC1D4 triggers the binding of this effector to inhibitory 14-3-3 proteins, which is required for insulin-stimulated glucose transport. AKT regulates also the storage of glucose in the form of glycogen by phosphorylating GSK3A at 'Ser-21' and GSK3B at 'Ser-9', resulting in inhibition of its kinase activity. Phosphorylation of GSK3 isoforms by AKT is also thought to be one mechanism by which cell proliferation is driven. AKT regulates also cell survival via the phosphorylation of MAP3K5 (apoptosis signal-related kinase). Phosphorylation of 'Ser-83' decreases MAP3K5 kinase activity stimulated by oxidative stress and thereby prevents apoptosis. AKT mediates insulin-stimulated protein synthesis by phosphorylating TSC2 at 'Ser-939' and 'Thr-1462', thereby activating mTORC1 signaling and leading to both phosphorylation of 4E-BP1 and in activation of RPS6KB1. AKT is involved in the phosphorylation of members of the FOXO factors (Forkhead family of transcription factors), leading to binding of 14-3-3 proteins and cytoplasmic localization. In particular, FOXO1 is phosphorylated at 'Thr-24', 'Ser-256' and 'Ser-319'. FOXO3 and FOXO4 are phosphorylated on equivalent sites. AKT has an important role in the regulation of NF-kappa-B-dependent gene transcription and positively regulates the activity of CREB1 (cyclic AMP (cAMP)-response element binding protein). The phosphorylation of CREB1 induces the binding of accessory proteins that are necessary for the transcription of pro-survival genes such as BCL2 and MCL1. AKT phosphorylates 'Ser-454' on ATP citrate lyase (ACLY), thereby potentially regulating ACLY activity and fatty acid synthesis. Activates the 3B isoform of cyclic nucleotide phosphodiesterase (PDE3B) via phosphorylation of 'Ser-273', resulting in reduced cyclic AMP levels and inhibition of lipolysis. Phosphorylates PIKFYVE on 'Ser-318', which results in increased PI3P-5 activity. The Rho GTPase-activating protein DLC1 is another substrate and its phosphorylation is implicated in the regulation cell proliferation and cell growth. AKT plays a role as key modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including correct neuron positioning, dendritic development and synapse formation. Signals downstream of phosphatidylinositol 3-kinase (PI3K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). AKT mediates the antiapoptotic effects of IGF-I. Essential for the SPATA13-mediated regulation of cell migration and adhesion assembly and disassembly. May be involved in the regulation of the placental development. Phosphorylates STK4/MST1 at 'Thr-120' and 'Thr-387' leading to inhibition of its: kinase activity, nuclear translocation, autophosphorylation and ability to phosphorylate FOXO3. Phosphorylates STK3/MST2 at 'Thr-117' and 'Thr-384' leading to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation. Phosphorylates SRPK2 and enhances its kinase activity towards SRSF2 and ACIN1 and promotes its nuclear translocation. Phosphorylates RAF1 at 'Ser-259' and negatively regulates its activity. Phosphorylation of BAD stimulates its pro-apoptotic activity. Phosphorylates KAT6A at 'Thr-369' and this phosphorylation inhibits the interaction of KAT6A with PML and negatively regulates its acetylation activity towards p53/TP53.AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility; prohibitin (PHB), playing an important role in cell metabolism and proliferation; and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

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Proteins (24)

Type

Over-Expression Cell Culture Supernatant (1)

Over-Expression Lysate (9)

Recombinant (14)

Target

AKT1 (24)

Publications

No (24)

Tag

6His, N-terminus (1)

8His, N-terminus (1)

GST (2)

GST, N-terminus (2)

His (2)

His, N-terminus (1)

His,C-terminus (1)

His-T7 (1)

Myc-DDK (Flag) (11)

Myc-DDK (Flag), C-terminus (2)

Species

Human (20)

Source

293T Cells (4)

E. coli (4)

HEK 293 Cells (11)

Insect Cells (2)

Wheat Germ Extract (3)

Purification

Purified (4)

Low Endotoxin Level

Low endotoxin level (2)

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Human AKT1 Protein (Recombinant His, N-terminus) - LS-G146201

Source:

E. coli

Tag:

His, N-terminus

Molecular Weight:

55.7kDa

Price:

20 µg/$270; 100 µg/$390

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Human AKT1 Protein (Recombinant GST) (Full Length) - LS-G22680

Source:

E. coli

Tag:

GST

Molecular Weight:

83.1 kD

Price:

100 µg/$420; 1 mg/$1,355; 20 µg/$323

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Human AKT1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G78518

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

55.5 kDa

Price:

20 µg/$215

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Human AKT1 Protein (Recombinant GST, N-terminus) (aa127-200) - LS-G57737

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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Human AKT1 Protein (Recombinant GST, N-terminus) (aa381-480) - LS-G57738

Source:

Wheat Germ Extract

Tag:

GST, N-terminus

Price:

10 µg/$479; 25 µg/$670

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Human AKT1 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G66797

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

55.5 kDa

Price:

20 µg/$1,107

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Human AKT1 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G66799

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

55.5 kDa

Price:

20 µg/$1,107

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Human AKT1 Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G66800

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

55.5 kDa

Price:

20 µg/$1,107

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Human AKT1 Protein (Recombinant His-T7) (aa122-443) - LS-G14910

Source:

E. coli

Tag:

His-T7

Molecular Weight:

40.9 kDa

Price:

10 µg/$277; 50 µg/$420; 100 µg/$670; 200 µg/$837; 1 mg/$1,928; 500 µg/$1,474; 5 mg/$2,927; 2 mg/$2,113

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Human AKT1 Protein (Over-Expression Lysate 8His, N-terminus) (Ser2-Ala480) - LS-G26712

Source:

HEK 293 Cells

Tag:

8His, N-terminus

Molecular Weight:

57 kDa

Price:

100 µg/$298

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Human AKT1 Protein (Recombinant GST) (Full Length) - LS-G27410

Source:

Wheat Germ Extract

Tag:

GST

Molecular Weight:

78.54 kDa

Price:

10 µg/$479; 25 µg/$670

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Human AKT1 Protein (Over-Expression Cell Culture Supernatant 6His, N-terminus) (Ser2-Ala480) - LS-G26854

Source:

HEK 293 Cells

Tag:

6His, N-terminus

Molecular Weight:

57 kDa

Price:

200 µl/$283

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AKT1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G100463

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

55.5 kDa

Price:

100 µg/$494

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Human AKT1 Protein (Recombinant His,C-terminus) (Met1-Ala480) - LS-G139928

Source:

Insect Cells

Tag:

His,C-terminus

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Molecular Weight:

57 kDa

Price:

50 µg/$661

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AKT1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G123625

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

55.5 kDa

Price:

100 µg/$494

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Human AKT1 Protein (Recombinant Myc-DDK (Flag), C-terminus) - LS-G142185

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag), C-terminus

Molecular Weight:

55.5 kDa

Price:

20 µg/$1,107

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Human AKT1 Protein (Recombinant Myc-DDK (Flag), C-terminus) - LS-G142189

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag), C-terminus

Molecular Weight:

55.7 kDa

Price:

20 µg/$1,107

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Human AKT1 Protein (Recombinant His) - LS-G3354

Source:

Insect Cells

Tag:

His

Endotoxin Level:

Less than 1.0 EU/µg protein (determined by LAL method).

Price:

50 µg/$361; 250 µg/$985; 10 µg/$261

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Human AKT1 Protein (Recombinant His) - LS-G3146

Source:

E. coli

Tag:

His

Price:

100 µg/$361; 500 µg/$739; 20 µg/$261

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Human AKT1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G78517

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

55.5 kDa

Price:

20 µg/$150

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Human AKT1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G78519

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

55.5 kDa

Price:

20 µg/$150

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Human AKT1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G78520

Source:

293T Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

55.5 kDa

Price:

20 µg/$150

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AKT1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G121569

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

55.5 kDa

Price:

100 µg/$494

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AKT1 Protein (Over-Expression Lysate Myc-DDK (Flag)) (Full Length) - LS-G121570

Source:

HEK 293 Cells

Tag:

Myc-DDK (Flag)

Molecular Weight:

55.5 kDa

Price:

100 µg/$710

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