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ACHE / Acetylcholinesterase

acetylcholinesterase

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally.

Gene Name: acetylcholinesterase
Synonyms: ACHE, ACEE, Acetylcholinesterase, N-ACHE, Yt blood group, ARACHE, YT
Target Sequences: NM_015831 P22303 P22303

Publications (1)

1
Impaired sweating in patients with cholinergic urticaria is linked to low expression of acetylcholine receptor CHRM3 and acetylcholine esterase in sweat glands. Yiyu Wang, Jörg Scheffel, Carolina Ayala Vera, Wei Liu, Dorothee Günzel, Dorothea Terhorst-Molawi, Marcus Maurer, Sabine Altrichter. Frontiers in immunology. 2022 August;13:955161. [Full Text Article] [PubMed:35967390] [PMC:PMC9373796] Related Antibodies: LS-B6676.

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Proteins (16)
Over-Expression Lysate (6)
Recombinant (10)
ACHE / Acetylcholinesterase (16)
No (16)
GST (2)
GST, N-terminus (1)
His (6)
Myc-DDK (Flag) (7)
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Human (7)
Mouse (3)
Rat (2)
293T Cells (3)
E. coli (6)
HEK 293 Cells (4)
Human Cells (1)
Wheat Germ Extract (2)
Purified (2)
Low endotoxin level (1)
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E. coli
His
24.2 kDa
10 µg/$289; 50 µg/$492; 100 µg/$785; 200 µg/$982; 1 mg/$2,119; 500 µg/$1,646; 5 mg/$3,431; 2 mg/$2,309
ACHE / Acetylcholinesterase Protein - 12.5% SDS-PAGE Stained with Coomassie Blue.
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Wheat Germ Extract
GST, N-terminus
10 µg/$479; 25 µg/$670
ACHE / Acetylcholinesterase Protein - Purified recombinant protein ACHE was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
60.8 kDa
20 µg/$1,107
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293T Cells
Myc-DDK (Flag)
68.2 kDa
20 µg/$150
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293T Cells
Myc-DDK (Flag)
67.38 kDa
20 µg/$150
ACHE / Acetylcholinesterase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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293T Cells
Myc-DDK (Flag)
68.2 kDa
20 µg/$150
ACHE / Acetylcholinesterase Protein - Recombinant Acetylcholinesterase By SDS-PAGE
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E. coli
His
23.9 kDa
10 µg/$287; 50 µg/$482; 100 µg/$770; 200 µg/$962; 1 mg/$2,094; 500 µg/$1,623; 5 mg/$3,361; 2 mg/$2,284
Select
E. coli
His
22.7 kDa
10 µg/$289; 50 µg/$492; 100 µg/$785; 200 µg/$982; 1 mg/$2,119; 500 µg/$1,646; 5 mg/$3,431; 2 mg/$2,309
Select
E. coli
His
20.2 kDa
10 µg/$289; 50 µg/$492; 100 µg/$785; 200 µg/$982; 1 mg/$2,119; 500 µg/$1,646; 5 mg/$3,431; 2 mg/$2,309
Select
E. coli
His
22.1 kDa
10 µg/$289; 50 µg/$492; 100 µg/$785; 200 µg/$982; 1 mg/$2,119; 500 µg/$1,646; 5 mg/$3,431; 2 mg/$2,309
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E. coli
GST
91.8 kD
1 mg/$1,679; 100 µg/$598; 20 µg/$405
ACHE / Acetylcholinesterase Protein - 12.5% SDS-PAGE of human ACHE stained with Coomassie Blue
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Wheat Germ Extract
GST
96.6 kDa
2 µg/$439
ACHE / Acetylcholinesterase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
68.2 kDa
100 µg/$494
ACHE / Acetylcholinesterase Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
68.2 kDa
100 µg/$494
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HEK 293 Cells
Myc-DDK (Flag)
67.38 kDa
100 µg/$494
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Human Cells
His
Less than 1.0 EU/µg protein (determined by LAL method).
66.2 kDa
20 µg/$649
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).