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ACAT1

acetyl-CoA acetyltransferase 1

The ACAT1 mRNA encodes a mitochondrially localized enzyme that catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. The ACAT gene spans approximately 27 kb and contains 12 exons interrupted by 11 introns. Defects in this gene are associated with the alpha-methylacetoaceticaciduria disorder, an inborn error of isoleucine catabolism characterized by urinary excretion of 2-methyl-3-hydroxybutyric acid, 2-methylacetoacetic acid, tiglylglycine, and butanone.

Gene Name: acetyl-CoA acetyltransferase 1
Synonyms: ACAT1, ACAT, Acetoacetyl-CoA thiolase, Acetyl-CoA acetyltransferase 1, MAT, T2, THIL
Target Sequences: NM_000019 NP_000010.1 P24752

Publications (2)

1
Regulation and immunolocalization of acyl-coenzyme A: cholesterol acyltransferase in mammalian cells as studied with specific antibodies. Chang CC, Chen J, Thomas MA, Cheng D, Del Priore VA, Newton RS, Pape ME, Chang TY. The Journal of biological chemistry. 1995 270:29532-40. [PubMed:7493995]
2
Androgens augment renal vascular responses to ANG II in New Zealand genetically hypertensive rats. Song J, Kost CK, Martin DS. American journal of physiology. Regulatory, integrative and comparative physiology. 2006 290:R1608-15. [PubMed:16469840]

☰ Filters
Products
Antibodies (54)
Primary (54)
ACAT1 (54)
Human (46)
Mouse (30)
Rat (18)
Bat (8)
Bovine (3)
Chicken (7)
Chimpanzee (6)
Dog (8)
Gibbon (6)
Guinea pig (6)
Hamster (8)
Horse (7)
Monkey (9)
Pig (1)
Rabbit (8)
Xenopus (6)
Zebrafish (3)
IHC (20)
IHC-P (13)
WB (49)
ELISA (13)
ICC (9)
IF (4)
IP (11)
Peptide-ELISA (7)
rabbit (46)
mouse (4)
goat (4)
Product Group
IHCPlus (7)
IgG (20)
IgG1,k (1)
IgG2a (1)
IgG2a,k (1)
monoclonal mc (4)
polyclonal pc (50)
2B7 (1)
9H10AB4 (1)
AT1.H11 (1)
AT2C5 (1)
APC (2)
APC Conjugated (2)
Biotin Conjugated (7)
Cy3 Conjugated (2)
Cy7 Conjugated (2)
FITC Conjugated (5)
HRP Conjugated (5)
PE Conjugated (2)
Unconjugated (29)
aa31-424 (8)
aa143-192 (4)
aa218-267 (4)
N-Terminus (2)
190-270 aa, Internal (1)
Internal (1)
Lys266 (1)
aa1-145 (1)
aa217-266 (1)
aa221-270 (1)
aa253-266 (1)
aa257-269 (1)
aa296-329 (1)
aa393-426 (1)
No (54)
ACAT1 Antibody - Anti-ACAT1 antibody IHC of human adrenal cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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ACAT1 Mouse anti-Human Monoclonal (N-Terminus) (AT1.H11) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$460
ACAT1 Antibody - Western blot of ACAT-1 antibody
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ACAT1 Rabbit anti-Human Polyclonal (190-270 aa, Internal) Antibody
Mouse, Rat, Human
ELISA, IHC, WB
Unconjugated
50 µg/$295; 100 µg/$335; 200 µg/$394
ACAT1 Antibody - Anti-ACAT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
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ACAT1 Rabbit anti-Human Polyclonal (aa296-329) Antibody
Human
IHC, IHC-P, WB
Unconjugated
200 µl/$460
ACAT1 Antibody - Anti-ACAT1 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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ACAT1 Rabbit anti-Human Polyclonal (aa218-267) Antibody
Human
IHC, IHC-P, WB
Unconjugated
100 µl/$485
ACAT1 Antibody - ACAT1 antibody (0.1 ug/ml) staining of Mouse Liver lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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ACAT1 Goat anti-Human Polyclonal (aa253-266) Antibody
Chicken, Rabbit, Mouse, Dog, Rat, Hamster, Horse, Bat, Human, Monkey
IF, Peptide-ELISA, WB
Unconjugated
100 µg/$503
ACAT1 Antibody - Anti-ACAT1 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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ACAT1 Mouse anti-Human Monoclonal (AT2C5) Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µl/$460
ACAT1 Antibody - Anti-ACAT1 antibody IHC of human liver, hepatocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
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ACAT1 Rabbit anti-Human Polyclonal (aa221-270) Antibody
Mouse, Rat, Human
IHC, IHC-P, Peptide-ELISA, WB
Unconjugated
50 µl/$460
ACAT1 Antibody - Anti-ACAT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.
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ACAT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Pig, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$460
ACAT1 Antibody - Anti-ACAT1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2 ug/ml.
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ACAT1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µl/$460
ACAT1 Antibody - Immunohistochemistry of paraffin-embedded human colon cancer at dilution of 1:100
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ACAT1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$294; 100 µg/$360
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ACAT1 Mouse anti-Human Monoclonal (9H10AB4) Antibody
Human
ELISA, ICC, IHC, IP
Unconjugated
100 µg/$471
ACAT1 Antibody - Immunohistochemistry of paraffin-embedded human liver tissue using antibody at 1:100 dilution.
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ACAT1 Rabbit anti-Human Polyclonal (aa1-145) Antibody
Mouse, Rat, Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µl/$294; 100 µl/$360
ACAT1 Antibody - Immunohistochemistry of paraffin-embedded human liver injury tissue.
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ACAT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IF, IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
ACAT1 Antibody - Immunohistochemical analysis of ACAT1 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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ACAT1 Rabbit anti-Human Polyclonal (Internal) Antibody
Mouse, Rat, Human, Monkey
IHC, IHC-P, IP, WB
Unconjugated
100 µl/$299
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ACAT1 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, WB
Unconjugated
100 µg/$331
ACAT1 Antibody - Staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
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ACAT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
ACAT1 Antibody - ACAT1 antibody (0.01 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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ACAT1 Goat anti-Human Polyclonal (aa257-269) Antibody
Rabbit, Mouse, Dog, Rat, Hamster, Bat, Human, Monkey
IF, Peptide-ELISA, WB
Unconjugated
100 µg/$503
ACAT1 Antibody - Knockout Varification: Lane 1: Wild-type A549 cell lysate; Lane 2: ACAT1 knockout A549 cell lysate; Predicted MW: 17,45kd Observed MW: 44kd Primary Ab: 1µg/ml Rabbit Anti-Human ACAT1 Antibody Second Ab: 0.2µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
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ACAT1 Rabbit anti-Human Polyclonal Antibody
Human
ICC, IP, WB
Unconjugated
100 µl/$309; 200 µl/$351; 500 µl/$491; 1 ml/$561; 2 ml/$755; 5 ml/$1,225
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ACAT1 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
ICC, IP, WB
Biotin Conjugated
100 µl/$319; 200 µl/$365; 500 µl/$519; 1 ml/$596; 2 ml/$824; 5 ml/$1,348
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ACAT1 Rabbit anti-Human Polyclonal (FITC) Antibody
Human
ICC, IP, WB
FITC Conjugated
100 µl/$348; 200 µl/$407; 500 µl/$605; 1 ml/$755; 2 ml/$993; 5 ml/$1,716
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ACAT1 Rabbit anti-Human Polyclonal (Cy3) Antibody
Human
ICC, IP, WB
Cy3 Conjugated
100 µl/$407; 200 µl/$491; 500 µl/$850; 1 ml/$1,008; 2 ml/$1,308; 5 ml/$2,451
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ACAT1 Rabbit anti-Human Polyclonal (APC) Antibody
Human
ICC, IP, WB
APC Conjugated
100 µl/$407; 200 µl/$491; 500 µl/$850; 1 ml/$1,008; 2 ml/$1,308; 5 ml/$2,451
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ACAT1 Rabbit anti-Human Polyclonal (Lys266) Antibody
Mouse, Rat, Human
IHC, WB
Unconjugated
50 µl/$321; 100 µl/$403
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ACAT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IHC, WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
ACAT1 Antibody - Immunohistochemistry of paraffin-embedded human liver injury using ACAT1 antibody at dilution of 1:100 (40x lens).
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ACAT1 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IHC, WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).