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15-PGDH / HPGD

hydroxyprostaglandin dehydrogenase 15-(NAD)

15-PGDH / HPGD is a member of the short-chain nonmetalloenzyme alcohol dehydrogenase protein family. The encoded enzyme is responsible for the metabolism of prostaglandins, which function in a variety of physiologic and cellular processes such as inflammation. Mutations in this gene result in primary autosomal recessive hypertrophic osteoarthropathy and cranioosteoarthropathy. Multiple transcript variants encoding different isoforms have been found for this gene.

Gene Name: hydroxyprostaglandin dehydrogenase 15-(NAD)
Synonyms: HPGD, 15-PGDH, PGDH, PGDH1, PHOAR1, Prostaglandin dehydrogenase 1, SDR36C1
Target Sequences: NM_000860 NP_000851.2 P15428

Publications (13)

1
Monoclonal antibodies that inhibit the enzyme activity of NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase. Tai CL, Mak OT, Arai T, Tai HH. The Biochemical journal. 1990 267:75-8. (Human) [PubMed:1691637] [PMC:PMC1131246]
2
Synergistic induction of the nicotinamide adenine dinucleotide-linked 15-hydroxyprostaglandin dehydrogenase by an androgen and interleukin-6 or forskolin in human prostate cancer cells. Tong M, Tai HH. Endocrinology. 2004 145:2141-7. [PubMed:14749354]
3
NAD+-linked 15-hydroxyprostaglandin dehydrogenase (15-PGDH) behaves as a tumor suppressor in lung cancer. Ding Y, Tong M, Liu S, Moscow JA, Tai HH. Carcinogenesis. 2005 26:65-72. [PubMed:15358636]
4
15-Hydroxyprostaglandin dehydrogenase is down-regulated in colorectal cancer. Backlund MG, Mann JR, Holla VR, Buchanan FG, Tai HH, Musiek ES, Milne GL, Katkuri S, DuBois RN. The Journal of biological chemistry. 2005 280:3217-23. [PubMed:15542609] [PMC:PMC1847633]
5
15-Hydroxyprostaglandin dehydrogenase, a COX-2 oncogene antagonist, is a TGF-beta-induced suppressor of human gastrointestinal cancers. Yan M, Rerko RM, Platzer P, Dawson D, Willis J, Tong M, Lawrence E, Lutterbaugh J, Lu S, Willson JK, Luo G, Hensold J, Tai HH, Wilson K, Markowitz SD. Proceedings of the National Academy of Sciences of the United States of America. 2004 101:17468-73. [PubMed:15574495] [PMC:PMC536023]
6
Repression of prostaglandin dehydrogenase by epidermal growth factor and snail increases prostaglandin E2 and promotes cancer progression. Mann JR, Backlund MG, Buchanan FG, Daikoku T, Holla VR, Rosenberg DW, Dey SK, DuBois RN. Cancer research. 2006 66:6649-56. [PubMed:16818638]
7
Repression of 15-hydroxyprostaglandin dehydrogenase involves histone deacetylase 2 and snail in colorectal cancer. Backlund MG, Mann JR, Holla VR, Shi Q, Daikoku T, Dey SK, DuBois RN. Cancer research. 2008 68:9331-7. [PubMed:19010907] [PMC:PMC2628771]
8
NAD+-dependent 15-hydroxyprostaglandin dehydrogenase regulates levels of bioactive lipids in non-small cell lung cancer. Hughes D, Otani T, Yang P, Newman RA, Yantiss RK, Altorki NK, Port JL, Yan M, Markowitz SD, Mazumdar M, Tai HH, Subbaramaiah K, Dannenberg AJ. Cancer prevention research (Philadelphia, Pa.). 2008 1:241-9. [PubMed:19138967] [PMC:PMC2720569]
9
Bile acids inhibit NAD+-dependent 15-hydroxyprostaglandin dehydrogenase transcription in colonocytes. Miyaki A, Yang P, Tai HH, Subbaramaiah K, Dannenberg AJ. American journal of physiology. Gastrointestinal and liver physiology. 2009 297:G559-66. [PubMed:19608733] [PMC:PMC2739822]
10
UV radiation inhibits 15-hydroxyprostaglandin dehydrogenase levels in human skin: evidence of transcriptional suppression. Judson BL, Miyaki A, Kekatpure VD, Du B, Gilleaudeau P, Sullivan-Whalen M, Mohebati A, Nair S, Boyle JO, Granstein RD, Subbaramaiah K, Krueger JG, Dannenberg AJ. Cancer prevention research (Philadelphia, Pa.). 2010 3:1104-11. (WB; Human) [PubMed:20643784] [PMC:PMC2933319]
11
Limited effect of anti-rheumatic treatment on 15-prostaglandin dehydrogenase in rheumatoid arthritis synovial tissue. Gheorghe KR, Sadique S, Leclerc P, Idborg H, Wobst I, Catrina AI, Jakobsson PJ, Korotkova M. Arthritis research & therapy. 2012 14:R121. (ICC, IHC-Fr; Human) [PubMed:22616846] [PMC:PMC3446502]
12
Prognostic implication of 15-hydroxyprostaglandin dehydrogenase down-regulation in patients with colorectal cancer. Kang PS, Kim JH, Moon OI, Lim SC, Kim KJ. Journal of the Korean Society of Coloproctology. 2012 28:253-8. (IHC; Human) [PubMed:23185705] [PMC:PMC3499426]
13
Three-dimensional culture system identifies a new mode of cetuximab resistance and disease-relevant genes in colorectal cancer. Li C, Singh B, Graves-Deal R, Ma H, Starchenko A, Fry WH, Lu Y, Wang Y, Bogatcheva G, Khan MP, Milne GL, Zhao S, Ayers GD, Li N, Hu H, Washington MK, Yeatman TJ, McDonald OG, Liu Q, Coffey RJ. Proceedings of the National Academy of Sciences of the United States of America. 2017 114:E2852-E2861. (WB; Human) [Full Text Article] [PubMed:28320945] [PMC:PMC5389279] Related Antibodies: LS-C172619.
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Proteins (13)
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Recombinant (9)
15-PGDH / HPGD (13)
No (13)
6His, C-terminus (2)
6His, N-terminus (1)
GST (1)
His (3)
His, N-terminus + His, C-terminus (1)
Myc-DDK (Flag) (5)
Human (9)
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293T Cells (2)
E. coli (5)
HEK 293 Cells (3)
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15-PGDH / HPGD Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
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HEK 293 Cells
Myc-DDK (Flag)
19.1 kDa
100 µg/$494
15-PGDH / HPGD Protein - Purified recombinant protein HPGD was analyzed by SDS-PAGE gel and Coomassie Blue Staining
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HEK 293 Cells
Myc-DDK (Flag)
28.8 kDa
20 µg/$1,107
15-PGDH / HPGD Protein - Recombinant Mouse Hpgd protein (His Tag)
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E. coli
His, N-terminus + His, C-terminus
20.3kDa
20 µg/$270; 100 µg/$390
15-PGDH / HPGD Protein - 12.5% SDS-PAGE of human HPGD stained with Coomassie Blue
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Wheat Germ Extract
GST
55 kDa
10 µg/$479; 25 µg/$670
15-PGDH / HPGD Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
28.8 kDa
20 µg/$150
15-PGDH / HPGD Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
293T Cells
Myc-DDK (Flag)
19.1 kDa
20 µg/$150
Select
E. coli
His
30.6 kDa
50 µg/$721
15-PGDH / HPGD Protein - Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Select
HEK 293 Cells
Myc-DDK (Flag)
28.8 kDa
100 µg/$494
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E. coli
His
29.7 kDa
50 µg/$854
15-PGDH / HPGD Protein
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E. coli
His
50 µg/$361; 250 µg/$739; 10 µg/$261; 20 µg/$298; 100 µg/$454; 500 µg/$1,177
15-PGDH / HPGD Protein
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E. coli
6His, N-terminus
31.3 kDa
10 µg/$265; 100 µg/$365
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Mammalian Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
30.0 kDa
10 µg/$344; 50 µg/$584
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Human Cells
6His, C-terminus
Less than 1.0 EU/µg protein (determined by LAL method).
30kD
10 µg/$321; 50 µg/$534; 500 µg/$1,585; 1 mg/$2,157
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The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).