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  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
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LSBio Publications

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The following are LSBio publications organized by topic. Here you can find white papers, press releases, and target overviews, and product references. LSBio white papers provide researchers with valuable scientific information resulting from research conducted in-house in the fields of immunohistochemistry and the generation and evaluation of antibodies for use in immunohistochemistry. Press releases are issued to announce significant product or business development events or milestones. In an effort to simplify navigation through those products associated with protein targets that have many family members, LSBio provides target overviews. Finally, LSBio is continually trying to collect publication references for papers in which LSBio products or services are used.
White Papers Press Releases Target Overviews Product References

LifeSpan's Immunohistochemistry (IHC) Validation

by Dr. Glenna Burmer April 12, 2011

LSBio (LifeSpan BioSciences) offers a catalog of 130,000 antibodies that have been tested for use in a variety of research applications, including immunohistochemistry, ELISA, Western blot, and flow cytometry. Antibodies can often be used in multiple assays, but they do not perform equally well in all assays. This is particularly true for immunohistochemistry (IHC). Many antibodies that perform well in other assays do not work well in IHC against formalin-fixed paraffin-embedded tissues (FFPE-IHC). In immunohistochemistry, antibodies may produce no signal, produce a weak signal, show nonspecific background staining that interferes with analysis, or show false positive signals. LSBio's goal in immunohistochemistry validation is to identify for our customers those antibodies that perform well in FFPE-IHC. Out of the 130,000 antibodies in the LSBio catalog, 35,000 have been tested and received validation for use in IHC by LSBio or through collaborators or suppliers. Of these, 9,900 antibodies have been extensively tested in our Seattle laboratory and awarded IHC-plusTM brand validation. IHC-plusTM antibodies have been identified as the best reagents for use in FFPE-IHC.[More]

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