LSBio The Immunohistochemistry Antibody Company
Products
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  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
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  • About Our IHC-plus™ Protocol
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  • Login to Our Secure FTP Site
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Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

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Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
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LSBio Rewards Program

We want to hear from you about how you’re using our antibodies! Click on a link below to send us a review in order to be eligible to receive a $20 coffee card!
 
Our goal is to gather end-user information about how our antibodies perform in various applications in order to help other researchers determine whether or not it may work for them.
 
Keep in mind that any data submitted may be published on our website. We also have the right to accept or refuse any submissions, or cancel this offer at any time.
 
       Select the application for which you would like to submit to review:
 
       Western Blot
       Flow Cytometry
       Other
 
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Sample Review

 
Application:
Sample Type:
Sample Fixative:
Antigen Retrieval:
Permeabilization:
Blocking:
Primary Ab Dilution:
Primary Ab Incubation Buffer:
Primary Ab Incubation Time:
Primary Ab Incubation Temp:
Secondary Ab Name:
Secondary Ab Host:
Secondary Ab Clonality:
Secondary Ab Conjugation:
Secondary Dilution:
Detection Method:
Immunohistochemistry (PFA perfusion fixed frozen sections)
Mouse brain, cortex
10% Neutral-buffered formalin
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
None
Vector Avidin/Biotin blocking kit (SP-2001) – 20 minutes at room temperature
1:200
PBS + BSA 3% + Triton-X100 0.2%
2 hours
4C
Vector biotinylated anti-rabbit BA-1400
Goat
Polyclonal
Biotin
1:1000
VECTASTAIN Elite ABC Kit PK-7200
 
Image:
 
       mouse_brain_sections
 
Caption: 4 dorsal ventricular sections through the mouse brain immunolabeled with an LSBio IHC-plus antibody.
 

 


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