Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Granulins are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. The 88 kDa precursor protein, progranulin, is also called proepithelin and PC cell-derived growth factor. Cleavage of the signal peptide produces mature granulin which can be further cleaved into a variety of active, 6 kDa peptides. These smaller cleavage products are named granulin A, granulin B, granulin C, etc.
Deglycosylation of rat progranulin. To examine the deglycosylation of rat Progranulin-FLAG, 1 ug of rat progranulin is denatured with 1X glycoprotein denaturing buffer at 100 degrees C for 10 minutes. After the addition of NP-40 and G7 reaction buffer, two-fold dilutions of PNGase F are added and the reaction mix is incubated for 1 or 3 hours at 37 degrees C. Separation of reaction products is visualized by immunoblotting using anti-FLAG-HRP antibody.