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Pyrococcus furiosus Pfu DNA Polymerase Recombinant Protein LS-G17485

Ordering

Wt. Vol. Conc. Price
100 U - - $255
500 U - - $325
2500 U - - $980
Inquire for larger quantities
LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Pyrococcus furiosus Pfu DNA Polymerase Recombinant Protein - LS-G17485
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LS-G17485
Recombinant Protein
Pfu DNA Polymerase
Pyrococcus furiosus
Pfu DNA Polymerase is a thermo-stable enzyme having a Mw of about 90kDa. Pfu DNA Polymerase is derived from E. coli that and cloned from Pyrococcus furiosus strain Vc1 DSM3638. Pfu DNA Polymerase replicates DNA at 75°C, catalyzing the polymerization of nucleotides into duplex DNA in the 5?´ to 3?´ direction in the existence of magnesium. Pfu DNA Polymerase possesses 3?´ to 5?´ exonuclease (proofreading) activity. Base misinsertions that take place during polymerization are swiftly removed by the proofreading activity of the polymerase. Therefore, Pfu DNA Polymerase is suggested for use in PCR and primer extension reactions that require high-fidelity synthesis. Pfu DNA Polymerase-generated PCR fragments are blunt-ended.
E. coli
E. coli
None
50 mM Tris-HCl, pH 8.2, 50 mM DTT, 50 mM EDTA, 0.05% CHAPS, 50% glycerol.
Store at -20°C. Avoid freeze/thaw cycles.
For research use only.

About Pfu DNA Polymerase


Requested From: United States
Date Requested: 12/9/2016

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