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Pyrococcus furiosus DUT / DUTPase Recombinant Protein LS-G17501

Ordering

Wt. Vol. Conc. Price
500 IU - - $335
1000 IU - - $435
2000 IU - - $785
Inquire for larger quantities
LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular DUT / DUTPase Proteins

Human DUT / DUTPase Recombinant (His) Protein - LS-G17787
E. coli Expression System
His
Unpurified / Lyophilized
Human DUT / DUTPase Recombinant Protein - LS-G39649
E. coli Expression System
None
Unpurified / Lyophilized
Human DUT / DUTPase Recombinant (6His,N-terminus) Protein - LS-G51862
E. coli Expression System
6His,N-terminus
Unpurified / Lyophilized

100% Guaranteed 100% Guaranteed
LS-G17501
Recombinant Protein
DUT / DUTPase
Pyrococcus furiosus
Thermostable dUTPase (pyrococcus fruriosus) maximizes the efficiency of high-fidelity PCR (using proofreading DNA polymerases). It removes contaminating dUTP present in PCR reactions and dNTP solutions. The presence of dUTPase in a proofreading DNA polymerase reaction can prevent dUTP misincorporation by maintaining dUTP levels below their inhibitory concentrations despite the constant generation of the molecule by the spontaneous deamination of dCTP. The incorporation of dUTP into PCR products causes mutations within the amplified product, proofreading polymerases to stall and slows down non-proofreading polymerases such as Taq. The dUTPase increase in PCR product yield, length and fidelity enables further down-stream applications. These effects make dUTPase useful in PCR fidelity and yield-sensitive applications such as cloning and subsequent recombinant protein technology, and gene expression analysis (semi-quantitative RT-PCR techniques and real-time PCR analysis), where small differences in product accumulation can have a significant impact on gene expression analysis. dUTPase is specific for dUTP and is critical for the fidelity of DNA replication and repair. dUTPase hydrolyzes dUTP to dUMP and pyrophosphate, simultaneously reducing dUTP levels and providing the dUMP for dTTP biosynthesis.
E. coli
E. coli
None
Greater than 97% by SDS-PAGE
20 mM Tris-HCl, pH 8.2, 20 mM DTT, 20 mM EDTA, 20 mM KCl, 0.1% Nonidet P40, 0.1% Tween 20, 50% glycerol
Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About DUT / DUTPase

P33316 NM_001948 NP_001939.1

DUT Protein, Deoxyuridine triphosphatase Protein, DUTP pyrophosphatase Protein, DUT-N Protein, DUTP nucleotidohydrolase Protein, DUTPase Protein

DUT / DUTPase is an essential enzyme of nucleotide metabolism. The encoded protein forms a ubiquitous, homotetrameric enzyme that hydrolyzes dUTP to dUMP and pyrophosphate. This reaction serves two cellular purposes: providing a precursor (dUMP) for the synthesis of thymine nucleotides needed for DNA replication, and limiting intracellular pools of dUTP.

Requested From: United States
Date Requested: 12/2/2016

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