Target
PD-L2 / PDCD1LG2 / CD273
Synonyms
PDCD1LG2 | B7 dendritic cell molecule | B7-DC | B7DC | BA574F11.2 | CD273 | Btdc | CD273 antigen | PD-L2 | PDCD1 ligand 2 | PD-1-ligand 2 | Programmed death ligand 2 | Butyrophilin B7-DC | PD-1 ligand 2 | PDCD1L2 | PDL2
Conjugations
Unconjugated
Applications
- Binding
- ELISA
- Functional Assay
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Predicted Molecular Weight
The recombinant rat PDCD1LG2 comprises 211 amino acids and predicts a molecular mass of 23.9 kDa. The apparent molecular mass of the recombinant protein is approximately 35.7 kDa in SDS-PAGE under reducing conditions.
Expression System
HEK 293 Cells
Purification
> 95 % as determined by SDS-PAGE.
Bio-Activity
Measured by its binding ability in a functional ELISA.Immobilized rat PDCD1LG2-His at 10 µg/ml (100 µl/well) can bind rat PDCD1-Fc, The EC50 of rat PDCD1-Fc is 15-35 ng/ml.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About PD-L2 / PDCD1LG2 / CD273