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| Catalog Number | Size | Price |
|---|---|---|
| LS-G47978-50 | 50 µg | $519 |
| LS-G47978-1 | 1 mg | $2,377 |
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Human NT5E / eNT / CD73 Protein (Recombinant His,C-terminus) (Met1-Lys547) - LS-G47978
Human NT5E / eNT / CD73 Protein (Recombinant His,C-terminus) (Met1-Lys547) - LS-G47978
Description:
NT5E / eNT / CD73 Protein LS-G47978 is a Recombinant Human NT5E / eNT / CD73 produced in HEK 293 Cells Met1-Lys547 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Description:
NT5E / eNT / CD73 Protein LS-G47978 is a Recombinant Human NT5E / eNT / CD73 produced in HEK 293 Cells Met1-Lys547 with His,C-terminus tag(s). It is biologically active and is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
NT5E / eNT / CD73
Synonyms
NT5E | 5-nucleotidase, ecto (CD73) | 5'-nucleotidase | 5 nucleotidase (CD73) | 5-NT | 5-nucleotidase | 5'-nucleotidase, ecto (CD73) | CD73 | CD73 antigen | E5NT | Ecto-5-nucleotidase | ENT | EN | NT | Purine 5-Prime-Nucleotidase | NT5 | NTE
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
His,C-terminus
Region
Met1-Lys547
Predicted Molecular Weight
The secreted recombinant human NT5E consists of 532 amino acids with the predicted molecular mass of 59.2 kDa.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 85 % as determined by SDS-PAGE
Bio-Activity
Measured by its ability to hydrolyze the 5’phosphate group from the substrate adenosine 5’monophosphate (AMP).The specific activity is > 20,000 pmoles/min/µg.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile 20mM Tris, 120 mM NaCl, 4 mM CaCl2, 20 % glycerol, pH 7.5, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About NT5E / eNT / CD73
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Binding
Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) bound MEDI9447 was 28 nM.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) bound MEDI9447 was 28 nM.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) bound MEDI9447 was 28 nM.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Binding
Using the Octet RED System, the affinity constant (Kd) of CD73 Protein, Human, Recombinant (His Tag) bound MEDI9447 was 28 nM.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular NT5E / eNT / CD73 Proteins
Source:
Mammal
Tag:
6His, C-terminus
Source:
E. coli
Tag:
His-T7
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
