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Bovine TMPRSS15 / Enterokinase Protein (Recombinant 6His,C-terminus) - LS-G27030

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Catalog
Size
Price
LS-G27030-10
10 µg
$215
LS-G27030-50
50 µg
$235
LS-G27030-1
1 mg
$860
Description
TMPRSS15 / Enterokinase Protein LS-G27030 is Recombinant Bovine TMPRSS15 / Enterokinase in Pichia pastoris with 6His,C-terminus tag(s). It is biologically active and is low in endotoxin Less than 0.2 EU/µg protein (determined by LAL method)..
Type
Recombinant Protein
Target
TMPRSS15 / Enterokinase
Species
Bovine
Predicted Molecular Weight
40 kDa (SDS-PAGE)
Details
Enterokinase (EK) is an enzyme produced by cells of the duodenum and involved in human digestion. It plays a role of turning trypsinogen to its active form trypsin, and indirectly activates the pancreatic digestive enzymes. Enterokinase is a specific protease that cleaves after a lysine preceded by four aspartic acids: Asp-Asp-Asp-Asp-Lys(DDDDK(up)). Enterokinase will not work if the recognition site is followed by a proline. rbEKhas the highest activity than EK of other species and is used widely in biochemical applications. rbEK with 6 x His-tag binds with Ni2+ affinity chromatography and was designed for removing from digestion system. Recombinant Bovine Enterokinase (His-tagged) (rbEK) as the light chain is a single glycosylated polypeptide chain containing 200 amino acids, 6 x His at C-terminus. A fully biologically active molecule, rbEK has a molecular mass of 40 kDa and is obtained by chromatographic techniques.
Expression System
Yeast
Source Species
Pichia pastoris
Tag
6His,C-terminus
Purification
Greater than 95% by SDS-PAGE
Bio-Activity
100 IU/µg
Endotoxin
Less than 0.2 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from a 0.2 um filtered solution in 20 mM Tris-HCl, pH 7.4, 200 mM NaCl, 2 mM CaCl2.
Reconstitution
Reconstitute lyophilized EK powder in sterile deionized water at 1mg/ml, then dilute with the provided EK Dilution/Storage Buffer according to your requirement.
Storage
Store at -20°C, stable for 1 year. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About TMPRSS15 / Enterokinase
P98073 NM_002772 NP_002763.2

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Requested From: United States
Date Requested: 9/21/2019