Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The tumor necrosis factor (TNF) and TNF receptor (TNFR) gene superfamilies regulate numerous biological functions including cell proliferation, differentiation, and survival through regulating the activation of the transcription factor NF-kB and various mitogen-activated protein kinases. The glucocorticoid-induced tumor necrosis factor receptor (GITR) is an emerging member of this family that is expressed on CD4+ CD25+ regulatory T cells and appears to have crucial immune regulation functions.
GITRL, Soluble (mouse) (rec.) induced GITR-mediated NF-kappaB activation. HEK293 cells were transiently transfected with 20ng of a (NF-kappaB)-Luc reporter, 2ng of a Renilla luciferase, and with 20ng of a GITR full length encoding construct. At 24 hours after transfection, the indicated amounts of GITRL, Soluble (mouse) (rec.) or a FLAG control protein were treated for 4 hours and followed by the measurement of dual luciferase activities.