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Catalog Number Size Price
LS-G3721-50 50 µg $446 
TNFSF18 / GITRL Protein - GITRL, Soluble (mouse) (rec.) induced GITR-mediated NF-kappaB activation. HEK293 cells were transiently transfected with 20ng of a (NF-kappaB)-Luc reporter, 2ng of a Renilla luciferase, and with 20ng of a GITR full length encoding construct. At 24 hours after transfection, the indicated amounts of GITRL, Soluble (mouse) (rec.) or a FLAG control protein were treated for 4 hours and followed by the measurement of dual luciferase activities.

Mouse TNFSF18 / GITRL Protein (Recombinant DDK (Flag)) (aa45-173) - LS-G3721

Mouse TNFSF18 / GITRL Protein (Recombinant DDK (Flag)) (aa45-173) - LS-G3721

Description:
TNFSF18 / GITRL Protein LS-G3721 is a Recombinant Mouse TNFSF18 / GITRL produced in HEK 293 Cells aa 45-173 with DDK (Flag) tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method).
Price
Catalog Number
$446
LS-G3721-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Description:
TNFSF18 / GITRL Protein LS-G3721 is a Recombinant Mouse TNFSF18 / GITRL produced in HEK 293 Cells aa 45-173 with DDK (Flag) tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method).

Specifications

Type
Recombinant Protein
Target
TNFSF18 / GITRL
Synonyms
TNFSF18 | AITR ligand | AITRL | GITR ligand | GITRL | HGITRL | TL6
Species
Mouse
Modifications
Unmodified
Conjugations
Unconjugated
Tag
DDK (Flag)
Region
aa 45-173
Predicted Molecular Weight
~22kDa (SDS-PAGE)
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 90% by SDS-PAGE
Bio-Activity
Induces GITR-mediated NF-kappaB activation in HEK 293 cells.
Endotoxin
Less than 0.1 EU/µg protein (determined by LAL method).
Presentation
10 mM Glycine, pH 10.0
Storage
Store at 4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNFSF18 / GITRL
Q9UNG2 NM_005092 NP_005083.2

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Functional Assay

TNFSF18 / GITRL Protein - GITRL, Soluble (mouse) (rec.) induced GITR-mediated NF-kappaB activation. HEK293 cells were transiently transfected with 20ng of a (NF-kappaB)-Luc reporter, 2ng of a Renilla luciferase, and with 20ng of a GITR full length encoding construct. At 24 hours after transfection, the indicated amounts of GITRL, Soluble (mouse) (rec.) or a FLAG control protein were treated for 4 hours and followed by the measurement of dual luciferase activities.
GITRL, Soluble (mouse) (rec.) induced GITR-mediated NF-kappaB activation. HEK293 cells were transiently transfected with 20ng of a (NF-kappaB)-Luc reporter, 2ng of a Renilla luciferase, and with 20ng of a GITR full length encoding construct. At 24 hours after transfection, the indicated amounts of GITRL, Soluble (mouse) (rec.) or a FLAG control protein were treated for 4 hours and followed by the measurement of dual luciferase activities.

Functional Assay

TNFSF18 / GITRL Protein - GITRL, Soluble (mouse) (rec.) induced GITR-mediated NF-kappaB activation. HEK293 cells were transiently transfected with 20ng of a (NF-kappaB)-Luc reporter, 2ng of a Renilla luciferase, and with 20ng of a GITR full length encoding construct. At 24 hours after transfection, the indicated amounts of GITRL, Soluble (mouse) (rec.) or a FLAG control protein were treated for 4 hours and followed by the measurement of dual luciferase activities.
GITRL, Soluble (mouse) (rec.) induced GITR-mediated NF-kappaB activation. HEK293 cells were transiently transfected with 20ng of a (NF-kappaB)-Luc reporter, 2ng of a Renilla luciferase, and with 20ng of a GITR full length encoding construct. At 24 hours after transfection, the indicated amounts of GITRL, Soluble (mouse) (rec.) or a FLAG control protein were treated for 4 hours and followed by the measurement of dual luciferase activities.

Functional Assay

TNFSF18 / GITRL Protein - GITRL, Soluble (mouse) (rec.) induced GITR-mediated NF-kappaB activation. HEK293 cells were transiently transfected with 20ng of a (NF-kappaB)-Luc reporter, 2ng of a Renilla luciferase, and with 20ng of a GITR full length encoding construct. At 24 hours after transfection, the indicated amounts of GITRL, Soluble (mouse) (rec.) or a FLAG control protein were treated for 4 hours and followed by the measurement of dual luciferase activities.
GITRL, Soluble (mouse) (rec.) induced GITR-mediated NF-kappaB activation. HEK293 cells were transiently transfected with 20ng of a (NF-kappaB)-Luc reporter, 2ng of a Renilla luciferase, and with 20ng of a GITR full length encoding construct. At 24 hours after transfection, the indicated amounts of GITRL, Soluble (mouse) (rec.) or a FLAG control protein were treated for 4 hours and followed by the measurement of dual luciferase activities.

Functional Assay

TNFSF18 / GITRL Protein - GITRL, Soluble (mouse) (rec.) induced GITR-mediated NF-kappaB activation. HEK293 cells were transiently transfected with 20ng of a (NF-kappaB)-Luc reporter, 2ng of a Renilla luciferase, and with 20ng of a GITR full length encoding construct. At 24 hours after transfection, the indicated amounts of GITRL, Soluble (mouse) (rec.) or a FLAG control protein were treated for 4 hours and followed by the measurement of dual luciferase activities.
GITRL, Soluble (mouse) (rec.) induced GITR-mediated NF-kappaB activation. HEK293 cells were transiently transfected with 20ng of a (NF-kappaB)-Luc reporter, 2ng of a Renilla luciferase, and with 20ng of a GITR full length encoding construct. At 24 hours after transfection, the indicated amounts of GITRL, Soluble (mouse) (rec.) or a FLAG control protein were treated for 4 hours and followed by the measurement of dual luciferase activities.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/20/2021