Target
LIF
Synonyms
LIF | DIA | Emfilermin | HILDA | Melanoma-derived LPL inhibitor | MLPLI | Leukemia inhibitory factor | CDF | D factor | Human interleukin in DA cells
Conjugations
Unconjugated
Predicted Molecular Weight
19.9 kDa (SDS-PAGE)
AA Sequence
SPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAFSPLPITPVNATCAIRHPCHGNLMNQIKNQLAQLNGSANALFISYYTAQGEPFPNNVEKLCAPNMTDFPSFHGNGTEKTKLVELYRMVAYLSASLTNITRDQKVLNPTAVSLQVKLNATIDVMRGLLSNVLCRLCNKYRVGHVDVPPVPDHSDKEAFQRKKLGCQLLGTYKQVISVVVQAF
Expression System
E. coli
Purification
Greater than 95% by non-reducing SDS-PAGE and HPLC
Bio-Activity
ED50 < 0.01 ng/mL, as determined by a cell differentiation assay using M1 cells, corresponding to a specific activity of > 1.0x 10^8 units/mg.
Endotoxin
Less than 0.2 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from 50 mM Tris-HCl, pH 8.0, 150 mM NaCl
Storage
Stable at -80°c for up to 6 months. Upon reconstitution, store at 4°c for up to 2 weeks or at -20°c for up to 3 months.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee