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| Catalog Number | Size | Price |
|---|---|---|
| LS-G3763-10 | 10 µg | $302 |
| LS-G3763-50 | 50 µg | $484 |
Mouse IL-33 Protein (Recombinant His) (aa109-266) - LS-G3763
Mouse IL-33 Protein (Recombinant His) (aa109-266) - LS-G3763
Description:
IL-33 Protein LS-G3763 is a Recombinant Mouse IL-33 produced in E. coli aa 109-266 with His tag(s). It is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Description:
IL-33 Protein LS-G3763 is a Recombinant Mouse IL-33 produced in E. coli aa 109-266 with His tag(s). It is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
IL-33
Synonyms
IL33 | C9orf26 | DVS27 | DVS27-related protein | IL-1F11 | IL-33 | Interleukin-33 | NFEHEV | NF-HEV | Interleukin-1 family member 11 | NFHEV | IL1F11 | Interleukin 33 | RP11-575C20.2
Species
Mouse
Modifications
Unmodified
Conjugations
Unconjugated
Tag
His
Region
aa 109-266
Predicted Molecular Weight
~20kDa (SDS-PAGE)
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 90% by SDS-PAGE
Bio-Activity
Activates mouse and human ST2-dependent NF-kappaB pathway.
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Storage
Store at 4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About IL-33
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Functional Assay
Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (mouse) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.
Functional Assay
Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (mouse) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.
Functional Assay
Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (mouse) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.
Functional Assay
Activation of a human ST2-dependent NF-kappaB pathway. HEK-293 cells were transiently co-transfected with 500 ng of human ST2-containing vector (or empty vector as a negative control), a vector containing NF-kappaB-driven firefly luciferase, and a vector containing Renilla luciferase as a internal control in a 12-well plate. 40 hours after transfection, cells were treated with IL33 (mouse) (rec.) (His) at the concentration as indicated for 8 hours, followed by measurement with a dual luciferase assay.
Popular IL-33 Proteins
Source:
E. coli
Tag:
His, N-Terminal
Source:
Human
Tag:
Myc-DDK (Flag)
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/23/2024
Date Requested: 4/23/2024
