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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Mouse IL-10 Protein (Recombinant) - LS-G134061

Catalog
Size
Price
LS-G134061-2
2 µg
$205
LS-G134061-10
10 µg
$285
LS-G134061-100
100 µg
$860
LS-G134061-500
500 µg
$3,555
LS-G134061-1
1 mg
$5,940
Description
IL-10 Protein LS-G134061 is Recombinant Mouse IL-10 in E. coli It is biologically active and is low in endotoxin Less than 1.0 EU/µg protein (determined by LAL method)..
Type
Animal-Free  Recombinant Protein
Target
IL-10
Species
Mouse
Predicted Molecular Weight
18.9 kDa
Details
Interleukin 10 (IL-10) is an anti-inflammatory cytokine produced by macrophages and type 2 T helper (Th2) cells. IL-10 inhibits the production of pro-inflammatory cytokines such as interferon gamma (IFN?), tumor necrosis factor alpha (TNFa), interleukin 2 (IL-2), interleukin 3 (IL-3), interleukin 4 (IL-4), and granulocyte-macrophage colony-stimulating factor (GM-CSF), made by macrophages and regulatory T cells. IL-10 also suppresses antigen presentation on antigen presenting cells, and enhances the survival, proliferation, and antibody production of B cells. Mouse IL-10 is not active on human cells.
AA Sequence
MSRGQYSREDNNCTHFPVGQSHMLLELRTAFSQVKTFFQTKDQLDNILLTDSLMQDFKGYLGCQALSEMIQFYLVEVMPQAEKHGPEIKEHLNSLGEKLKTLRMRLRRCHRFLPCENKSKAVEQVKSDFNKLQDQGVYKAMNEFDIFINCIEAYMMIKMKS
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 95% by reducing and non-reducing SDS-PAGE
Bio-Activity
Determined by MC/9 Cell Proliferation; <= 5 ng/ml; >= 2.0x10^5 units/mg
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from 10 mM Sodium Phosphate, pH 7.5
Reconstitution
Centrifuge vial before opening. Reconstitute with sterile water at 0.1 mg/mL. Suspend the product by gently pipetting the recommended solution down the sides of the vial. Do not vortex. Allow several minutes for complete reconstitution.
Storage
Shipped at room temperature. Prior to reconstitution, store at -20°C. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution. Store at -80°C and avoid freeze-thaw cycles.
Restrictions
For research use only.
About IL-10
P22301 NM_000572 NP_000563.1

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Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis Image
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Images

Assay Result

Mouse IL-10 Bioassay: Proliferation of MC/9 Cells
Mouse IL-10 Bioassay: Proliferation of MC/9 Cells

Western blot

Assay Result

Mouse IL-10 Bioassay: Proliferation of MC/9 Cells
Mouse IL-10 Bioassay: Proliferation of MC/9 Cells

Western blot

Assay Result

Mouse IL-10 Bioassay: Proliferation of MC/9 Cells
Mouse IL-10 Bioassay: Proliferation of MC/9 Cells

Western blot

Assay Result

Mouse IL-10 Bioassay: Proliferation of MC/9 Cells
Mouse IL-10 Bioassay: Proliferation of MC/9 Cells

Western blot


Requested From: United States
Date Requested: 7/18/2019
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