IL-10 Protein LS-G134061 is Recombinant Mouse IL-10 in E. coli It is biologically active and is low in endotoxin Less than 1.0 EU/µg protein (determined by LAL method)..
Predicted Molecular Weight
Interleukin 10 (IL-10) is an anti-inflammatory cytokine produced by macrophages and type 2 T helper (Th2) cells. IL-10 inhibits the production of pro-inflammatory cytokines such as interferon gamma (IFN?), tumor necrosis factor alpha (TNFa), interleukin 2 (IL-2), interleukin 3 (IL-3), interleukin 4 (IL-4), and granulocyte-macrophage colony-stimulating factor (GM-CSF), made by macrophages and regulatory T cells. IL-10 also suppresses antigen presentation on antigen presenting cells, and enhances the survival, proliferation, and antibody production of B cells. Mouse IL-10 is not active on human cells.
Greater than 95% by reducing and non-reducing SDS-PAGE
Determined by MC/9 Cell Proliferation; <= 5 ng/ml; >= 2.0x10^5 units/mg
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized from 10 mM Sodium Phosphate, pH 7.5
Centrifuge vial before opening. Reconstitute with sterile water at 0.1 mg/mL. Suspend the product by gently pipetting the recommended solution down the sides of the vial. Do not vortex. Allow several minutes for complete reconstitution.
Shipped at room temperature. Prior to reconstitution, store at -20°C. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution. Store at -80°C and avoid freeze-thaw cycles.
For research use only.