Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
FGF23 is a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR).
ERK and FRS2alpha phosphorylation induced by FGF-23 in Klotho expressing cells. Klotho expressing HEK 293EBNA cells were serum starved for 16 hours and then stimulated with mFGF-23-Fc (positive control) for 10 minutes, respectively. Antibodies against pFRS2alpha, pERK1/2 and total ERK1/2 were used for immunoblotting. Lane 1: Mock (non-treated) Lane 2: Klotho + mFGF23-Fc 1ng/ml Lane 3: Klotho + mFGF23-Fc 10ng/ml Lane 4: Klotho + mFGF23-Fc 100ng/ml Lane 5: Klotho + mFGF23-Fc 500ng/ml Lane 6: Klotho + mFGF23-Fc 1000ng/ml Lane 7: Klotho + 100ng/ml FGF-b