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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Catalog Number Size Price
LS-G136167-1 1 mg (1 mg/ml) $385 
EGF Protein - Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.

Mouse EGF Protein (Recombinant) - LS-G136167

Mouse EGF Protein (Recombinant) - LS-G136167

Description:
EGF Protein LS-G136167 is Recombinant Mouse EGF in E. coli It is biologically active
Price
Catalog Number
$385 
LS-G136167-1
1 mg
1 mg/ml
Toll Free North America
866-819-4732

Overview

Description:
EGF Protein LS-G136167 is Recombinant Mouse EGF in E. coli It is biologically active

Specifications

Type
Recombinant Protein
Target
EGF
Species
Mouse
Modifications
Unmodified
Predicted Molecular Weight
6 kDa
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 98% by HPLC and SDS PAGE
Bio-Activity
The ED50 as determined by a cell proliferation assay using BALB/c 3T3 cells is = 0.1 ng/ml, corresponding to a specific activity of = 1 x 10^7 units/mg.
Endotoxin
Not Tested
Reconstitution
Reconstitute with H2O to a concentration of 1.0 mg/ml. Gently mix after reconstitution. Do not vortex.
Storage
Store at -20°C. Do not use frost-free freezers. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About EGF
P01133 NM_001963 NP_001954.2

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Images

Western blot

EGF Protein - Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.
Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.

Western blot

EGF Protein - Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.
Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.

Western blot

EGF Protein - Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.
Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.

Western blot

EGF Protein - Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.
Western Blot analysis 1 µg recombinant mouse EGF was loaded in lanes 2 and 3, as the primary antibody Goat anti Mouse EGF antibody was used at 0.1 µg/ml and 0.2 µg/ml. HRP conjugated Donkey anti Sheep/Goat IgG antibody was used as the secondary antibody at a dilution of 1/20000 in lanes 2, 3 and 4. Bio-Rad Precision Plus™ Dual Xtra Prestained Protein Standards were run in lane 1. The blot was visualized with the Bio-Rad Chemidoc™ Touch Imaging System.

Requested From: United States
Date Requested: 12/14/2019