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Catalog Number Size Price
LS-G3829-10 10 µg $278 
LS-G3829-50 50 µg $369 
DLL4 Protein - Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.

Mouse DLL4 Protein (Recombinant Human IgG1 Fc) (aa1-532) - LS-G3829

Mouse DLL4 Protein (Recombinant Human IgG1 Fc) (aa1-532) - LS-G3829

Description:
DLL4 Protein LS-G3829 is a Recombinant Mouse DLL4 produced in HEK 293 Cells aa 1-532 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method).
Price
Catalog Number
$278
LS-G3829-10
Toll Free North America
866-819-4732
For Research Use Only

Overview

Description:
DLL4 Protein LS-G3829 is a Recombinant Mouse DLL4 produced in HEK 293 Cells aa 1-532 with Human IgG1 Fc tag(s). It is low in endotoxin; Less than 0.1 EU/µg protein (determined by LAL method).

Specifications

Type
Recombinant Protein
Target
DLL4
Synonyms
DLL4 | Delta-like 4 protein | Delta like 4 | Delta-like 4 (Drosophila) | Delta-like 4 homolog | Drosophila Delta homolog 4 | Notch ligand DLL4 | Delta 4 | Delta ligand 4 | Delta-like protein 4 | Delta4 | Hdelta2 | Notch ligand delta-2
Species
Mouse
Modifications
Unmodified
Conjugations
Unconjugated
Tag
Human IgG1 Fc
Region
aa 1-532
Predicted Molecular Weight
~95kDa (SDS-PAGE)
Expression System
HEK 293 Cells
Source Species
Human
Purification
Greater than 90% by SDS-PAGE
Bio-Activity
Inhibits adipogenesis of 3T3L-1 cells.
Endotoxin
Less than 0.1 EU/µg protein (determined by LAL method).
Presentation
PBS
Storage
Store at 4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About DLL4
Q9NR61 NM_019074 NP_061947.1

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Functional Assay

DLL4 Protein - Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.

Functional Assay

DLL4 Protein - Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.
Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL4-Fc (Prod. No. AG-40A-0145), hTNFalpha and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 6/17/2021