Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Acts as a ligand for Notch receptors. Blocks the differentiation of progenitor cells into the B-cell lineage while promoting the emergence of a population of cells with the characteristics of a T-cell/NK-cell precursor.
Adipogenesis inhibition of 3T3L1 cells. 3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1 uM Dexamethasone, 0.5mM IBMX, 10 ug/m lnsulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5 ug/ml mDLL1-Fc and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin, and stained with 0.5% filtered Oil Red O in propylene glycol.
Induction of Hes-1 with the treatment of recombinant mouse DLL1-Fc (Prod. No. AG-40A-0148). A mouse preadpipocyte cell line, 3T3L1, was stimulated with 5mg/ml of mouse DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH. Lane 1: mDLL1 (Fc protein), 0 minutes Lane 2: mDLL1 (Fc protein), 10 minutes Lane 3: mDLL1 (Fc protein), 30 minutes Lane 4: mDLL1 (Fc protein), 1 hours Lane 5: mDLL1 (Fc protein), 2 hours Lane 6: mDLL1 (Fc protein), 4 hours Lane 7: mDLL1 (Fc protein), 8 hours Lane 8: mDLL1 (Fc protein), 24 hours