Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Purified / Lyophilized / Endotoxin Level: Less than 1.0 EU/µg protein (determined by LAL method).
CTLA4 / CD152
The extracellular domain of mouse CD152 [CTLA-4] (aa 1-160) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.
Mouse mutant IgG2a Fc
Greater than 98% by SDS-PAGE
Blocks the binding of mouse CD80 (B7-1) and CD86 (B7-2) to their receptors (by binding CD80 and CD86 with high affinity) and thereby prevents their T cell regulatory actions by inhibiting the CD28 signaling competitively. Shows the biological functions of the CD152 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain. Useful for investigating the T cell co-stimulation.
Less than 0.06 EU/µg protein (determined by LAL method).
Lyophilized from PBS.
Store lyophilized at -20°C for at least 1 year. Once reconstituted store at +4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid repeat freeze-thaw cycles.
CTLA4 / CD152 is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer.