Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Purified / Lyophilized / Endotoxin Level: Less than 1.0 EU/µg protein.
Ctf2 / Cardiotrophin 2
Greater than 98% by SDS-PAGE and HPLC
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.5-0.8 ug/ml.
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Sterile filtered, lyophilized from 2.5 mM Tris, ph 10.2, 0.5 mM DTT
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid repeat freeze-thaw cycles.
Ctf2 / Cardiotrophin 2 is also know as Neuropoietin. The mouse Ctf2 gene is localized in tandem with the Ctf1 gene on chromosome 7. Neuropoietin is structurally and functionally related with CNTF, cardiotrophin-1, and cardiotrophin-like cytokine. High expression of Neuropoietin is observed in embryonic neuroepithelial cells. Expression is seen also in the retina and, to a lesser extent, in skeletal muscle. In vitro, Neuropoietin is a survival factor for embryonic motor neurons.