Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The ED50 as determined by the dose-dependent stimulation of the proliferation of human TF-1 cells is 0.5-0.8 ug/ml.
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Sterile filtered, lyophilized from 2.5 mM Tris, ph 10.2, 0.5 mM DTT
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid repeat freeze-thaw cycles.
Ctf2 / Cardiotrophin 2 is also know as Neuropoietin. The mouse Ctf2 gene is localized in tandem with the Ctf1 gene on chromosome 7. Neuropoietin is structurally and functionally related with CNTF, cardiotrophin-1, and cardiotrophin-like cytokine. High expression of Neuropoietin is observed in embryonic neuroepithelial cells. Expression is seen also in the retina and, to a lesser extent, in skeletal muscle. In vitro, Neuropoietin is a survival factor for embryonic motor neurons.