Target
Lysobacter Enzymogenes ArgC
Species
Lysobacter enzymogenes
Conjugations
Unconjugated
Predicted Molecular Weight
26.8 kDa
AA Sequence
GVGDIGSSDYCEKDIVCRVKPSAEFLSASKSVARMVFTPKTGYTGYCSGTLLNNSNSPKRQLFWSAAHCISTQKVANTLQTYWLYDATGCDNDTLSDKAVTLTGGATLLHSHATRDTLLLELKSAPPSGAYYAGWNSSAIATKGTAIEGIHHPSGDLKKYSLGSVTALSSTIDGKKPLTKVAWTTGVTEGGSSGSGLFTISSTSGYQLRGGLYGGTSYCSAPSDPDYYSQLDGVWSSIKTYFSPHHHHHHHH
Expression System
High Five Cells
Source Species
Trichoplusia ni
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
The reaction is measured as an increase in absorbance at 253 nm resulting from the hydrolysis of N-benzoyl-L-arginine ethyl ester (BAEE).
Endotoxin
Less than 0.1 ng/µg of protein (less than 1EU/µg).
Presentation
Sterile filtered, lyophilized from 50 mM Tris, pH 8.0, 10 mM calcium chloride
Reconstitution
Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Arg-C is activated by DTT Cysteine or other sulfhydryl containing reagents. The presence of calcium ions is essential. Arg-C is inhibited by oxidizing agents and sulfhydryl reactants and by Co2 Cu2 Cd2 and heavy metal ions.
Storage
If lyophilized, can be stored for 1 month at room temperature, 6 months at 4°C, or through the expiration date at -20°C to -80°C. Once reconstituted per the supplied instructions, can be stored for 3 months at -20°C to -80°C, or for 1 week at 2°C to 8°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee