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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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IL8 / Interleukin 8 Protein (Over-Expression Lysate Myc + DDK) (Full Length) - LS-G99083

Available for shipment within the USA only
Catalog
Size
Price
LS-G99083-100
100 µg
$365

Available for shipment within the USA only
Description
IL8 / Interleukin 8 Protein LS-G99083 is Over-Expression Lysate IL8 / Interleukin 8 in Human with Myc + DDK tag(s).
Type
Over-Expression Lysate Protein
Target
IL8 / Interleukin 8
Predicted Molecular Weight
11.1 kDa Full Length
Details
This product includes lysate of HEK293T cells that have been transiently-tranfected with Human IL8(CXCL8) (NM_000584), cells transfected with the vector alone, and a reducing buffer for SDS-Page gel loading. Cells were lysed using Modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix and 1mM Na3VO4).
AA Sequence
MTSKLAVALLAAFLISAALCEGAVLPRSAKELRCQCIKTYSKPFHPKFIKELRVIESGPHCANTEIIVKLSDGRELCLDPKENWVQRVVEKFLKRAENSTRTRPLEQKLISEEDLAANDILDYKDDDDKV
Expression System
HEK 293 Cells
Source Species
Human
Purification
Unpurified
Bio-Activity
Not Tested
Endotoxin
Not Tested
Storage
Store at -20°C for up to 1 year. Avoid freeze-thaw cycles.
Usage Summary
Western blot: Spin down briefly and dilute with 2xSDS Sample Buffer provided (4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromphenol blue, 100mM DTT). Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Restrictions
For research use only. Available for shipment within the USA only.
About IL8 / Interleukin 8
P10145 NM_000584 NP_000575.1

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Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis Image
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Images

Western blot

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Western blot

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Western blot

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Western blot

Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate
Western validation with an anti-DDK antibody * L: Control HEK293 lysate R: Over-expression lysate

Requested From: United States
Date Requested: 4/23/2019
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