Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The extracellular domain of human B7-H4 (aa 29-258) is fused to the N-terminus of the Fc region of human IgG1.
Human IgG1 Fc
Greater than 98% by SDS-PAGE
Measured by the ablility to inhibit anti-CD3-induced proliferation of stimulated human T cells. Human T lymphocytes cultured for 72 hours with PHA were incubated for an additional 3 days in 96 well place coated with 500ng/ml anti-CD3 and 10µg/ml human B7H4/Fc. The presence of human B7H4/Fc at 10µg/ml inhibited anti CD3 response by 30-50%. Optimal dilutions should be determined by each laboratory for each application.
Less than 0.06 EU/µg protein (determined by LAL method).
Lyophilized from PBS.
Store lyophilized at -20°C for at least 1 year. Once reconstituted store at +4°C for immediate use, or aliquot and store at -20°C for up to 3 months. Avoid repeat freeze-thaw cycles.
VTCN1 Protein, B7 superfamily member 1 Protein, B7 Superfamily, member 1 Protein, B7h.5 Protein, B7S1 Protein, B7H4 Protein, PRO1291 Protein, VCTN1 Protein, RP11-229A19.4 Protein, B7 family member, H4 Protein, B7-H4 Protein, B7X Protein, Protein B7S1 Protein
Negatively regulates T-cell-mediated immune response by inhibiting T-cell activation, proliferation, cytokine production and development of cytotoxicity. When expressed on the cell surface of tumor macrophages, plays an important role, together with regulatory T-cells (Treg), in the suppression of tumor-associated antigen-specific T-cell immunity. Involved in promoting epithelial cell transformation.