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Catalog Number Size Price
LS-G27306-10 10 µg $262 
LS-G27306-50 50 µg $304 
LS-G27306-1 1 mg $990 
TNF Alpha Protein - Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.

Human TNF Alpha Protein (Recombinant) - LS-G27306

Human TNF Alpha Protein (Recombinant) - LS-G27306

Description:
TNF Alpha Protein LS-G27306 is a Recombinant Human TNF Alpha produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.2 EU/µg protein (determined by LAL method). For Research Use Only
Price
Catalog Number
$262
LS-G27306-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Description:
TNF Alpha Protein LS-G27306 is a Recombinant Human TNF Alpha produced in E. coli. It is biologically active and is low in endotoxin; Less than 0.2 EU/µg protein (determined by LAL method). For Research Use Only

Specifications

Type
Recombinant Protein
Target
TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Predicted Molecular Weight
17.3 kDa (SDS-PAGE)
AA Sequence
VRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLFKGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKGDRLSAEINRPDYLDFAESGQVYFGIIAL
Expression System
E. coli
Source Species
E. coli
Purification
Greater than 98% by SDS-PAGE and HPLC
Bio-Activity
ED50 < 30 pg/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of > 3.3 x 10^7 units/mg.
Endotoxin
Less than 0.2 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from PBS
Storage
Stable at -80°c for up to 6 months. Upon reconstitution, store at 4°c for up to 1 week or at -20°c for up to 3 months.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This protein carries the LSBio 100% Guarantee.
LSBio Guarantee
About TNF Alpha
P01375 NM_000594 NP_000585.2

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Enzyme-Linked Immunosorbent Assay

TNF Alpha Protein - Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.
Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.

Enzyme-Linked Immunosorbent Assay

TNF Alpha Protein - Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.
Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.

Enzyme-Linked Immunosorbent Assay

TNF Alpha Protein - Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.
Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.

Enzyme-Linked Immunosorbent Assay

TNF Alpha Protein - Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.
Biological Activity TNF-alpha, Human stimulates cytotoxicity of L-929 mouse fibrosarcoma cells. The ED 50 for this effect is typically 11-50 pg/mL.

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024