TNF Alpha Protein LS-G23518 is Recombinant Human TNF Alpha in E. coli It is biologically active and is low in endotoxin Less than 1.0 EU/µg protein (determined by LAL method)..
Predicted Molecular Weight
Tumor necrosis factor alpha (TNFa) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNFa signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNFa functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever. This product is produced with no animal or human origin raw products. All processing and handling employs animal free equipment and animal free protocols.
Greater than 95% by SDS-PAGE
Tested using: Cytolysis of mouse L929 cells in the presence of Actinomycin D. Less than or equal to 2 ng/mL and greater than or equal to 5.0 x 10^5 units/mg.
Less than 1.0 EU/µg protein (determined by LAL method).
Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5
Reconstitute in sterile water to 0.1 mg/ml
Store at -20°C to -80°C for up to 1 year. Store at 4°C for up to 1 month after reconstitution.
For research use only.