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| Catalog Number | Size | Price |
|---|---|---|
| LS-G47237-100 | 100 µg | $441 |
| LS-G47237-1 | 1 mg | $2,003 |
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Human TNF Alpha Protein (Recombinant Human IgG1 Fc, N-terminus) (Val77-Leu233) - LS-G47237
Human TNF Alpha Protein (Recombinant Human IgG1 Fc, N-terminus) (Val77-Leu233) - LS-G47237
Description:
TNF Alpha Protein LS-G47237 is a Recombinant Human TNF Alpha produced in HEK 293 Cells Val77-Leu233 with Human IgG1 Fc, N-terminus tag(s). It is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Description:
TNF Alpha Protein LS-G47237 is a Recombinant Human TNF Alpha produced in HEK 293 Cells Val77-Leu233 with Human IgG1 Fc, N-terminus tag(s). It is low in endotoxin; Less than 1.0 EU/µg protein (determined by LAL method). For Research Use Only
Specifications
Type
Recombinant Protein
Target
TNF Alpha
Synonyms
TNF | APC1 protein | Cachectin | DIF | TNF Alpha | Tumor necrosis factor-alpha | TNF-alpha | TNF-a | TNFA | Tumor necrosis factor | TNF, macrophage-derived | TNF, monocyte-derived | TNFSF2
Species
Human
Modifications
Unmodified
Conjugations
Unconjugated
Applications
- Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Tag
Human IgG1 Fc, N-terminus
Region
Val77-Leu233
Predicted Molecular Weight
The recombinant human TNF consists 417 amino acids and predicts a molecular mass of 45.8 kDa.
Expression System
HEK 293 Cells
Source Species
Human
Purification
> 90 % as determined by SDS-PAGE
Bio-Activity
Not Tested
Endotoxin
Less than 1.0 EU/µg protein (determined by LAL method).
Presentation
Lyophilized from sterile PBS, pH 7.4, with 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Reconstitution
Please refer to included instructions for detailed information.
Storage
Aliquot and store at -20°C to -80°C for one year. Avoid repeated freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About TNF Alpha
Publications (0)
Customer Reviews (0)
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Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.5-3ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.5-3ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.5-3ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Cytotoxic Assay
Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D.The ED50 for this effect is typically 0.5-3ng/mL.
Sodium Dodecyl Sulfate - Polyacrylamide Gel Electrophoresis
Popular TNF Alpha Proteins
Source:
Wheat
Tag:
GST, N-terminus
Source:
Human
Tag:
His, N-Terminal
Source:
E. coli
Tag:
His, N-Terminal
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
Date Requested: 4/25/2024
